| module name: | UAS module |
| size: | 383 bp |
| cloning sites: | XbaI / ApaI |
| creation: | PCR cloning (pP{UAST} as template) |
| remarks: | for Gal4 inducible expression of cDNAs |
Ladder Map of Enzyme Sites:
# hits 30 60 90 120 150 180 210 240 270 300 330 360 : : : : : : : : : : : : ApaI --------------------------------------------------/ 1 BspMI -\------------------------------------------------- 1 EarI -----------------------------------------------\--- 1 NsiI /-------------------------------------------------- 1 : : : : : : : : : : : : PvuII -------------------------------|------------------- 1 SbfI --/------------------------------------------------ 1 SphI -/------------------------------------------------- 1 XbaI \-------------------------------------------------- 1 : : : : : : : : : : : : Bpu1102I ---------------------------\\---------------------- 2 PstI --/-------------------------------/---------------- 2 BsrBI ----|--|--|-|--|----------------------------------- 5 ScaI ---|-|--|-|--|------------------------------------- 5Linear Map of Sequence:
BspMI SphI PstI XbaI NsiI SbfI ScaI BsrBI ScaI BsrBI \ \\\ \ \ \ \ \ 1 tctagatgcatgcctgcaggtcggagtactgtcctccgagcggagtactgtcctccgagc 60 agatctacgtacggacgtccagcctcatgacaggaggctcgcctcatgacaggaggctcg ^ * ^ * ^ * ^ * ^ * ^ * ScaI BsrBI ScaI BsrBI ScaI BsrBI \ \ \ \ \ \ 61 ggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcgga 120 cctcatgacaggaggctcgcctcatgacaggaggctcgcctcatgacaggaggctcgcct ^ * ^ * ^ * ^ * ^ * ^ * 121 gactctagcgagcgccggagtataaatagaggcgcttcgtctacggagcgacaattcaat 180 ctgagatcgctcgcggcctcatatttatctccgcgaagcagatgcctcgctgttaagtta ^ * ^ * ^ * ^ * ^ * ^ * Bpu1102I Bpu1102I PvuII \ \ \ 181 tcaaacaagcaaagtgaacacgtcgctaagcgaaagctaagcaaataaacaagcgcagct 240 agtttgttcgtttcacttgtgcagcgattcgctttcgattcgtttatttgttcgcgtcga ^ * ^ * ^ * ^ * ^ * ^ * PstI \ 241 gaacaagctaaacaatctgcagtaaagtgcaagttaaagtgaatcaattaaaagtaacca 300 cttgttcgatttgttagacgtcatttcacgttcaatttcacttagttaattttcattggt ^ * ^ * ^ * ^ * ^ * ^ * EarI \ 301 gcaaccaagtaaatcaactgcaactactgaaatctgccaagaagtaattattgaatacaa 360 cgttggttcatttagttgacgttgatgactttagacggttcttcattaataacttatgtt ^ * ^ * ^ * ^ * ^ * ^ * ApaI \ 361 gaagagaactctgaatagggccc 420 cttctcttgagacttatcccggg ^ * ^ * ^ * ^ * ^ * ^ *