module name: rosy module
size: 7292 bp
cloning sites: HindIII / HindIII
creation: 7.2kb HindIII rosy fragment from pP{Car20}



 

Ladder Map of Enzyme Sites:

# hits 700 1400 2100 2800 3500 4200 4900 5600 6300 7000 : : : : : : : : : : AflII ------------------------------------------------\--- 1 AhdI -------------------------/-------------------------- 1 AlwNI ----------------------------------------/----------- 1 BbsI --------------------------\------------------------- 1 : : : : : : : : : : BciVI --/------------------------------------------------- 1 BplI --------------------|------------------------------- 1 BspLU11I ---------\------------------------------------------ 1 BsrGI ----------------------------------------------\----- 1 : : : : : : : : : : BssHII --------------------------------------\------------- 1 BstZ17I -----|---------------------------------------------- 1 DrdI -----------------/---------------------------------- 1 EcoNI --\------------------------------------------------- 1 : : : : : : : : : : EcoRI ---------------------\------------------------------ 1 EcoRV --------------------------------------------|------- 1 FspI ---------------|------------------------------------ 1 HgiEII -----------------------------------|---------------- 1 : : : : : : : : : : MluI -------------------\-------------------------------- 1 NdeI ------------------------------------\--------------- 1 NgoAIV ------------------------\--------------------------- 1 NspV -----------------------------------------------\---- 1 700 1400 2100 2800 3500 4200 4900 5600 6300 7000 : : : : : : : : : : Pfl1108I ---|------------------------------------------------ 1 PinAI ----------------------------------------------\----- 1 PmeI ----------------|----------------------------------- 1 PshAI ---------------------------------------------|------ 1 : : : : : : : : : : PvuI -------------------------------------------/-------- 1 SanDI -------------------------------\-------------------- 1 SbfI -/-------------------------------------------------- 1 ScaI -----------------------------|---------------------- 1 : : : : : : : : : : SexAI ----------------------\----------------------------- 1 SfiI ----------------------------/----------------------- 1 SgrAI ---------------------------------------\------------ 1 SnaBI -----|---------------------------------------------- 1 : : : : : : : : : : StuI ----------------------------|----------------------- 1 Tth111I ------------------------------------------\--------- 1 UbaDI ----------------------|----------------------------- 1 XhoI --------------------\------------------------------- 1 : : : : : : : : : : XmnI ------------------------------------------|--------- 1 AclI ---------------------------\----------\------------- 2 BcgI ------------------------/--------------------/------ 2 BcgI -----------------------/--------------------/------- 2 700 1400 2100 2800 3500 4200 4900 5600 6300 7000 : : : : : : : : : : BfiI -------------------\------\------------------------- 2 Bpu10I --------------\-\----------------------------------- 2 BsaXI ----------------------------|---------------|------- 2 BsmBI -\--------\----------------------------------------- 2 : : : : : : : : : : BspEI ------------------\-------\------------------------- 2 BspGI -------------------------------|--|----------------- 2 DraIII -------/------------------/------------------------- 2 HindIII \--------------------------------------------------\ 2 : : : : : : : : : : NcoI ------------------------------------\-----------\--- 2 NsiI ------------------------------------/-------------/- 2 PmlI |-----------------------------|--------------------- 2 PstI -/---------------------------/---------------------- 2 : : : : : : : : : : RcaI -------------------------\-----\-------------------- 2 SmaI --------------------------------------------|-|----- 2 SphI ---------------------------------------------/----/- 2 BglI ----------------------------/-------------/-/------- 3 : : : : : : : : : : Bpu1102I ----------------------------\---\-----------\------- 3 ClaI ------------\-----------\-------------------\------- 3 RleAI --------------/----------------------/----/--------- 3 SapI ---------------------\-------\----------------\----- 3 700 1400 2100 2800 3500 4200 4900 5600 6300 7000 : : : : : : : : : : SspI ------|------------------------------------------2-- 3 TaqII ---------/--------/--/------------------------------ 3 ApaLI -------------\----\-----\---\----------------------- 4 BamHI -------------------\-\-------------------\\--------- 4 : : : : : : : : : : BclI --------2---------------------------\----\---------- 4 BglII --\---\----------\---------\------------------------ 4 Bsp24I /------------------/---------------/----------/----- 4 Bsp24I /------------------/----------------/---------/----- 4 : : : : : : : : : : BsrBI ----------------------------|---|-------|-----|----- 4 BstEII -----------------\-\\-\----------------------------- 4 DrdII ----|------------------|-----------------|------|--- 4 MscI ---------|-----|---------|--|----------------------- 4 : : : : : : : : : : NarI ------------------\-------------\----------\---\---- 4 NruI |----------------------------------|---|-------|---- 4 PvuII ----------------------2-----------------|------|---- 4 SacI --------------/----------/--/---/------------------- 4 : : : : : : : : : : BsaI ---------\-----------\--\----\---\------------------ 5 BspMI -3-------\-----------------------------\------------ 5 BsrDI -------------------------------/-/---------/---/--/- 5 BstAPI -/-----------------/--------------/------------/--/- 5 700 1400 2100 2800 3500 4200 4900 5600 6300 7000 : : : : : : : : : : DraI -------|--------2-------------------------------||-- 5 EarI ---------------------\-------\-----\----------2----- 5 Bce83I ------/-2-----------------------/-----/--------/---- 6 BsbI --|-------|----|---------------------|---||--------- 6 : : : : : : : : : : BsgI ---------/--------/2/-------------/----------------- 6 BsmI ------------/-------/------------------------///--/- 6 BssSI ---------------\----------\-\--\-\----------------\- 6 EciI ------------------|--------||----2-------|---------- 6 : : : : : : : : : : MunI -\-----------2-\\---------------------------------\- 6 PflMI --------------/--/---/-----2------------/----------- 6 VspI ------\----------\------------------------------2\\- 6 BsaBI |-------|-------|-------||---------------|---|------ 7 : : : : : : : : : : BstXI /---------------------/--/-/------/-------/---/----- 7 Eco57I --/-----2-------------2/-----/---------------------- 7 XcmI ---/-------------------/---/---/-/---//------------- 7 BseRI ----------/-------/---/----/-/--//-------/---------- 8 : : : : : : : : : : TaqII /-/-----------/--------//-------------/---/---/----- 8 AceIII --------------\-------\\\---2\--\\---------------\-- 10 BpmI ----------------------//-/-----/-/------3--/-/-/-/-- 12

Linear Map of Sequence:

HindIII Bsp24I \ \ 1 aagctttaactatgtttaatctcccatagagcttcgttatcttcggacgcgatgtggcgc 60 ttcgaaattgatacaaattagagggtatctcgaagcaatagaagcctgcgctacaccgcg ^ * ^ * ^ * ^ * ^ * ^ * Bsp24I BsaBI \ \ 61 agatcccgtgctttcgtaacagttttctctacggaatcagcggagggatcggcatcggct 120 tctagggcacgaaagcattgtcaaaagagatgccttagtcgcctccctagccgtagccga ^ * ^ * ^ * ^ * ^ * ^ * PmlI NruI BstXI TaqII \ \ \ 121 tgctgacttttctgggcacctcgcgaacccacctgtccacccacgtgggcttcggctcct 180 acgactgaaaagacccgtggagcgcttgggtggacaggtgggtgcacccgaagccgagga ^ * ^ * ^ * ^ * ^ * ^ * PstI SbfI BspMI BspMI \ \ \ 181 tcttctgcggcaccatcgcctactggatgacctgcaggtaagtgactgtgattgaaaccg 240 agaagacgccgtggtagcggatgacctactggacgtccattcactgacactaactttggc ^ * ^ * ^ * ^ * ^ * ^ * BspMI \ 241 tgcgtcgcccttatcacttaattatcaatgtggcatccgcaggtatcaatggtccgtcag 300 acgcagcgggaatagtgaattaatagttacaccgtaggcgtccatagttaccaggcagtc ^ * ^ * ^ * ^ * ^ * ^ * MunI BstAPI BsmBI EcoNI \ \ \ \ 301 gagattcgagcagcagcaattgcgtgaggcgatgagacgacaagccctttatgagggcac 360 ctctaagctcgtcgtcgttaacgcactccgctactctgctgttcgggaaatactcccgtg ^ * ^ * ^ * ^ * ^ * ^ * BglII TaqII Eco57I \ \ \ 361 ccaaagggagagagacttagatctgaagtcggcgtagcataagtcagatccttcattact 420 ggtttccctctctctgaatctagacttcagccgcatcgtattcagtctaggaagtaatga ^ * ^ * ^ * ^ * ^ * ^ * BciVI \ 421 acttcgatgtttgttttcactctaggataccaagtaatcctggcattgtcaaatcccttg 480 tgaagctacaaacaaaagtgagatcctatggttcattaggaccgtaacagtttagggaac ^ * ^ * ^ * ^ * ^ * ^ * BsbI \ 481 tgttgtaaactaccttttgttaccaaactttatagtagccaatatattatatatatgttg 540 acaacatttgatggaaaacaatggtttgaaatatcatcggttatataatatatatacaac ^ * ^ * ^ * ^ * ^ * ^ * Pfl1108I \ 541 taagtgaaatcatagcttggagtttgcttgccactcgtagggaattagttatgccaccat 600 attcactttagtatcgaacctcaaacgaacggtgagcatcccttaatcaatacggtggta ^ * ^ * ^ * ^ * ^ * ^ * XcmI DrdII \ \ 601 ctttgatttgggattgacagaaatcaatccatcattttaatgattgtggttcctaaaacc 660 gaaactaaaccctaactgtctttagttaggtagtaaaattactaacaccaaggattttgg ^ * ^ * ^ * ^ * ^ * ^ * 661 tgttgggctttattgtgctttcgatttgatttaagctgatagagctagttgtttactttt 720 acaacccgaaataacacgaaagctaaactaaattcgactatctcgatcaacaaatgaaaa ^ * ^ * ^ * ^ * ^ * ^ * 721 gaagaacattgaacccattttgacgaccttttaactttttaatagtttttttttaccatt 780 cttcttgtaacttgggtaaaactgctggaaaattgaaaaattatcaaaaaaaaatggtaa ^ * ^ * ^ * ^ * ^ * ^ * SnaBI \ 781 tacataaaagcaaaatttacagtctaaaagttgtatattcaatacgttctggtacgtaaa 840 atgtattttcgttttaaatgtcagattttcaacatataagttatgcaagaccatgcattt ^ * ^ * ^ * ^ * ^ * ^ * BstZ17I \ 841 agggttatttaatgcaccatttttacaactttagatagtatactttttacttgagtggga 900 tcccaataaattacgtggtaaaaatgttgaaatctatcatatgaaaaatgaactcaccct ^ * ^ * ^ * ^ * ^ * ^ * Bce83I VspI \ \ 901 gccatatcacaaaaatgaaatgaaaggttaaaaaagtatctaaaagtaagtttcaattaa 960 cggtatagtgtttttactttactttccaattttttcatagattttcattcaaagttaatt ^ * ^ * ^ * ^ * ^ * ^ * SspI \ 961 tattctgaaaaataattgcatttcatgtacgtttctctgaaatacccctaattcgatact 1020 ataagactttttattaacgtaaagtacatgcaaagagactttatggggattaagctatga ^ * ^ * ^ * ^ * ^ * ^ * BglII \ 1021 atttatagatctggaagatatatagctaggaaatagaattatatatttgcctagtttgaa 1080 taaatatctagaccttctatatatcgatcctttatcttaatatataaacggatcaaactt ^ * ^ * ^ * ^ * ^ * ^ * DraIII DraI \ \ 1081 caccttgtgaacattttgtttaaaaaaaaagcgtatggtggtactagaaaactttaagat 1140 gtggaacacttgtaaaacaaatttttttttcgcataccaccatgatcttttgaaattcta ^ * ^ * ^ * ^ * ^ * ^ * 1141 acagataaataacttcactataccgaggttttggtgtttcttgagactgaaggacaatct 1200 tgtctatttattgaagtgatatggctccaaaaccacaaagaactctgacttcctgttaga ^ * ^ * ^ * ^ * ^ * ^ * BclI Bce83I Eco57I Bce83I Eco57I \ \ \ \\ 1201 tcaaaatggtcttaattcttgagatagaaaagctgaagttctcatgctgaatagtgatca 1260 agttttaccagaattaagaactctatcttttcgacttcaagagtacgacttatcactagt ^ * ^ * ^ * ^ * ^ * ^ * BclI BsaBI \ \ 1261 gtacgattttatctgacagattgctttcctagctgatcataccgatctcggatgggtatc 1320 catgctaaaatagactgtctaacgaaaggatcgactagtatggctagagcctacccatag ^ * ^ * ^ * ^ * ^ * ^ * BspMI MscI \ \ 1321 tgctctaatccgagatagcaggtagtagaactgctgcctcgtaacccctagacatggcca 1380 acgagattaggctctatcgtccatcatcttgacgacggagcattggggatctgtaccggt ^ * ^ * ^ * ^ * ^ * ^ * BsgI BspLU11I TaqII BsaI \ \ \ \ 1381 tttccacgcacatgtaggcgatcctgcactcgtcggtctctcgtcttatctcacaaaaat 1440 aaaggtgcgtgtacatccgctaggacgtgagcagccagagagcagaatagagtgttttta ^ * ^ * ^ * ^ * ^ * ^ * 1441 aaaagtcggcgtttggatggattgctcggctggctgttggcacattcattacgttttcgt 1500 ttttcagccgcaaacctacctaacgagccgaccgacaaccgtgtaagtaatgcaaaagca ^ * ^ * ^ * ^ * ^ * ^ * BsmBI BsbI \ \ 1501 tgacggcgcgagacggttgtgttgttttttaatcggcgggcacatgaggttgtcggttct 1560 actgccgcgctctgccaacacaacaaaaaattagccgcccgtgtactccaacagccaaga ^ * ^ * ^ * ^ * ^ * ^ * BseRI \ 1561 ctaattttgaaataaaaaacttgttttcgctttacattcaaggaacgcacctcctcccct 1620 gattaaaactttattttttgaacaaaagcgaaatgtaagttccttgcgtggaggagggga ^ * ^ * ^ * ^ * ^ * ^ * 1621 ccatatatgtaaacgagcttcgtaattgcaggctaacacttatataggcattggtactga 1680 ggtatatacatttgctcgaagcattaacgtccgattgtgaatatatccgtaaccatgact ^ * ^ * ^ * ^ * ^ * ^ * 1681 gatacagcgagataccgtgagctacagatacatagcacttcacgatgtctaactcggttt 1740 ctatgtcgctctatggcactcgatgtctatgtatcgtgaagtgctacagattgagccaaa ^ * ^ * ^ * ^ * ^ * ^ * 1741 tggtgtttttcgtgaatggaaagaaggtacttaaagatactcggatgccttgcgtgtctt 1800 accacaaaaagcacttacctttcttccatgaatttctatgagcctacggaacgcacagaa ^ * ^ * ^ * ^ * ^ * ^ * ClaI \ 1801 tccttaaagtcggcccactcttactatatcgattccctttattgttttattgccctaatt 1860 aggaatttcagccgggtgagaatgatatagctaagggaaataacaaaataacgggattaa ^ * ^ * ^ * ^ * ^ * ^ * BsmI MunI \ \ 1861 gcgtttaactggcattcgatggaatttgccttcttgctcaattgaatgtgtcgaatgtat 1920 cgcaaattgaccgtaagctaccttaaacggaagaacgagttaacttacacagcttacata ^ * ^ * ^ * ^ * ^ * ^ * MunI \ 1921 atagtatttgctgtgcatatagtgataacaattgacccccatgtgaagcctcgttgtttg 1980 tatcataaacgacacgtatatcactattgttaactgggggtacacttcggagcaacaaac ^ * ^ * ^ * ^ * ^ * ^ * ApaLI RleAI \ \ 1981 ctggtggccccttatcgcccgatcaagatggtgcacttgccaatcgcgcatgggagcagt 2040 gaccaccggggaatagcgggctagttctaccacgtgaacggttagcgcgtaccctcgtca ^ * ^ * ^ * ^ * ^ * ^ * TaqII AceIII Bpu10I \ \ \ 2041 gctactcgaatgtgggtgagctgggatcgtcatgggcatttgtgtctgccagatagctca 2100 cgatgagcttacacccactcgaccctagcagtacccgtaaacacagacggtctatcgagt ^ * ^ * ^ * ^ * ^ * ^ * PflMI SacI \ \ 2101 ggccctctaatccgcacattcccaccggctgggcggtgtttacaatccagtgagctcggc 2160 ccgggagattaggcgtgtaagggtggccgacccgccacaaatgttaggtcactcgagccg ^ * ^ * ^ * ^ * ^ * ^ * MscI BsbI MunI \ \ \ 2161 tggattattcagataaagctggccatatttagcatctggtgttggtggagtgcaattgga 2220 acctaataagtctatttcgaccggtataaatcgtagaccacaaccacctcacgttaacct ^ * ^ * ^ * ^ * ^ * ^ * FspI \ 2221 gttctttactttgtgctcacagcttgcgcacaagtgaagtaaataataataaataaccag 2280 caagaaatgaaacacgagtgtcgaacgcgtgttcacttcatttattattatttattggtc ^ * ^ * ^ * ^ * ^ * ^ * BssSI BsaBI Bpu10I \ \ \ 2281 ttgctcgtgcgcccggcatttgatagtgatagatagcaatctctgtttgctcagggctgg 2340 aacgagcacgcgggccgtaaactatcactatctatcgttagagacaaacgagtcccgacc ^ * ^ * ^ * ^ * ^ * ^ * DraI PmeI MunI \ \ 2341 cagagcagataacactttcgctgttttaagtttaaacaaagtcacagttttgacaaacaa 2400 gtctcgtctattgtgaaagcgacaaaattcaaatttgtttcagtgtcaaaactgtttgtt ^ * ^ * ^ * ^ * ^ * ^ * DraI BglII \ \ 2401 ttgctttaaaccatataaacttataattaaagcaattataatgggttaagtaagcacaga 2460 aacgaaatttggtatatttgaatattaatttcgttaatattacccaattcattcgtgtct ^ * ^ * ^ * ^ * ^ * ^ * VspI PflMI \ \ 2461 tctagctacaagatacaagatacaaattttccaattaatggataaatggcattagatact 2520 agatcgatgttctatgttctatgtttaaaaggttaattacctatttaccgtaatctatga ^ * ^ * ^ * ^ * ^ * ^ * BstEII \ 2521 tttgggaaattgatttcgtttttttcattttattatatttatacatttcttattccaggt 2580 aaaccctttaactaaagcaaaaaaagtaaaataatataaatatgtaaagaataaggtcca ^ * ^ * ^ * ^ * ^ * ^ * BseRI BspEI DrdI TaqII ApaLI \ \ \ \ \ 2581 gaccgaagtgtcgcctgatccggagtgcacgctcctcacattcctgcgcgaaaagctgcg 2640 ctggcttcacagcggactaggcctcacgtgcgaggagtgtaaggacgcgcttttcgacgc ^ * ^ * ^ * ^ * ^ * ^ * BsgI EciI NarI \ \ \ 2641 gctgtgcggaacgaagttgggatgtgcggaaggcggatgcggcgcctgcaccgtgatggt 2700 cgacacgccttgcttcaaccctacacgccttccgcctacgccgcggacgtggcactacca ^ * ^ * ^ * ^ * ^ * ^ * 2701 gtcccgcctagaccgtcgggccaacaagatacgtcacctggcggtcaacgcctgcctgac 2760 cagggcggatctggcagcccggttgttctatgcagtggaccgccagttgcggacggactg ^ * ^ * ^ * ^ * ^ * ^ * BstEII Bsp24I \ \ 2761 gccagtgtgctcgatgcacggatgtgcggtgaccactgtggagggtatagggagcaccaa 2820 cggtcacacgagctacgtgcctacacgccactggtgacacctcccatatccctcgtggtt ^ * ^ * ^ * ^ * ^ * ^ * BsgI BstAPI BfiI MluI Bsp24I BsgI BamHI \ \ \ \ \\ 2821 aacgcgtctgcatccggtgcaggagcgactgcccaaggcgcacggatcccagtgcggctt 2880 ttgcgcagacgtaggccacgtcctcgctgacgggttccgcgtgcctagggtcacgccgaa ^ * ^ * ^ * ^ * ^ * ^ * BplI \ 2881 ctgcacgccgggcattgtgatgtccatgtacgcacttctgcggaacgcggagcaaccctc 2940 gacgtgcggcccgtaacactacaggtacatgcgtgaagacgccttgcgcctcgttgggag ^ * ^ * ^ * ^ * ^ * ^ * BsgI BsmI BstEII \ \ \ 2941 tatgcgagacttggaggtggcattccaaggtaacctgtgccgctgcaccggctatcgacc 3000 atacgctctgaacctccaccgtaaggttccattggacacggcgacgtggccgatagctgg ^ * ^ * ^ * ^ * ^ * ^ * XhoI \ 3001 cattctcgagggctacaagacgttcaccaaggagtttgcctgcggaatgggcgagaagtg 3060 gtaagagctcccgatgttctgcaagtggttcctcaaacggacgccttacccgctcttcac ^ * ^ * ^ * ^ * ^ * ^ * EarI SapI BsaI TaqII \ \ \ 3061 ctgcaaagttagtgggaaaggatgtggaaccgatgcggagaccgatgacaagctcttcga 3120 gacgtttcaatcaccctttcctacaccttggctacgcctctggctactgttcgagaagct ^ * ^ * ^ * ^ * ^ * ^ * BamHI Eco57I EcoRI PflMI UbaDI \ \ \ \\ 3121 gcgcagcgaattccagcccttggatcccagccaggaacccatcttcccaccggaacttca 3180 cgcgtcgcttaaggtcgggaacctagggtcggtccttgggtagaagggtggccttgaagt ^ * ^ * ^ * ^ * ^ * ^ * SexAI PvuII BstEII \ \ \ 3181 gctgagtgacgccttcgattcgcagagtttgatctttagttcggatagggtgacctggta 3240 cgactcactgcggaagctaagcgtctcaaactagaaatcaagcctatcccactggaccat ^ * ^ * ^ * ^ * ^ * ^ * Eco57I PvuII AceIII BseRI BstXI BpmI Eco57I \ \\ \\ \ \ 3241 tcgtcccaccaatctggaggagctgcttcagctgaaggccaaacatccggctgccaagct 3300 agcagggtggttagacctcctcgacgaagtcgacttccggtttgtaggccgacggttcga ^ * ^ * ^ * ^ * ^ * ^ * XcmI \ 3301 ggtcgtgggcaatacggaagtgggcgttgaggttaagttcaagcacttcctctacccgca 3360 ccagcacccgttatgccttcacccgcaactccaattcaagttcgtgaaggagatgggcgt ^ * ^ * ^ * ^ * ^ * ^ * DrdII AceIII TaqII BcgI BpmI \ \ \ \ \ 3361 cctcatcaatcccacccaggtgaaggagctgctggagatcaaagagaaccaggatggcat 3420 ggagtagttagggtgggtccacttcctcgacgacctctagtttctcttggtcctaccgta ^ * ^ * ^ * ^ * ^ * ^ * ClaI BcgI BsaBI AceIII \ \ \ \ 3421 ttacttcggtgcggctgtcagtttgatggagatcgatgcgcttctgcggcagagaatcga 3480 aatgaagccacgccgacagtcaaactacctctagctacgcgaagacgccgtctcttagct ^ * ^ * ^ * ^ * ^ * ^ * BsaI ApaLI \ \ 3481 gctgctgccggaatcggagaccagattgttccagtgcaccgtggatatgcttcactactt 3540 cgacgacggccttagcctctggtctaacaaggtcacgtggcacctatacgaagtgatgaa ^ * ^ * ^ * ^ * ^ * ^ * NgoAIV TaqII RcaI AhdI \ \ \ \ 3541 tgccggcaagcagatccgcaacgtcgcctgtttgggtggaaacatcatgaccggcagtcc 3600 acggccgttcgtctaggcgttgcagcggacaaacccacctttgtagtactggccgtcagg ^ * ^ * ^ * ^ * ^ * ^ * BsaBI SacI MscI \ \ \ 3601 catttccgatatgaatcctgtgctctcggcagcaggagctcaactggaggtggccagttt 3660 gtaaaggctatacttaggacacgagagccgtcgtcctcgagttgacctccaccggtcaaa ^ * ^ * ^ * ^ * ^ * ^ * BstXI BpmI BfiI \ \ \ 3661 tgtggatggaaagctccaaaagagatcagttcacatgggaactgggttcttcactggcta 3720 acacctacctttcgaggttttctctagtcaagtgtacccttgacccaagaagtgaccgat ^ * ^ * ^ * ^ * ^ * ^ * DraIII BssSI BbsI \ \ \ 3721 tcgcaggaatgttatcgaagcccacgaggtgctgctgggcatccactttcggaagaccac 3780 agcgtccttacaatagcttcgggtgctccacgacgacccgtaggtgaaagccttctggtg ^ * ^ * ^ * ^ * ^ * ^ * BspEI \ 3781 tccggaccagtatatcgttgcttttaagcaggccagaagaagggatgatgacatagccat 3840 aggcctggtcatatagcaacgaaaattcgtccggtcttcttccctactactgtatcggta ^ * ^ * ^ * ^ * ^ * ^ * EciI AclI BstXI BglII \ \ \ \ 3841 cgtaaatgccgcaataaacgttcgctttgaggaaaaatccaacattgtggcggagatctc 3900 gcatttacggcgttatttgcaagcgaaactcctttttaggttgtaacaccgcctctagag ^ * ^ * ^ * ^ * ^ * ^ * XcmI BseRI PflMI \ \\ 3901 gatggcttttggtggaatggcaccaaccacagtgctcgctcctcgaacttcccaactgat 3960 ctaccgaaaaccaccttaccgtggttggtgtcacgagcgaggagcttgaagggttgacta ^ * ^ * ^ * ^ * ^ * ^ * AceIII BssSI ApaLI PflMI AceIII EciI BsaXI \ \ \ \ \ \\ 3961 ggttgggcaggagtggagccaccagctcgtggagcgcgtggcggagagcttgtgcacgga 4020 ccaacccgtcctcacctcggtggtcgagcacctcgcgcaccgcctctcgaacacgtgcct ^ * ^ * ^ * ^ * ^ * ^ * BsrBI SacI \ \ 4021 gctgcctttggctgcctccgctccgggtggcatgatcgcctatcgtcgagctctggtggt 4080 cgacggaaaccgacggaggcgaggcccaccgtactagcggatagcagctcgagaccacca ^ * ^ * ^ * ^ * ^ * ^ * MscI BglI StuI SfiI Bpu1102I Eco57I \ \ \ \ \ 4081 gagcctgttcttcaaggcctatctggccatttccctgaagctgagcaagtcagggatcac 4140 ctcggacaagaagttccggatagaccggtaaagggacttcgactcgttcagtccctagtg ^ * ^ * ^ * ^ * ^ * ^ * BseRI ScaI \ \ 4141 atcttccgatgctctgccaccggaggagcgaagtggtgccgagacattccacacaccagt 4200 tagaaggctacgagacggtggcctcctcgcttcaccacggctctgtaaggtgtgtggtca ^ * ^ * ^ * ^ * ^ * ^ * EarI SapI AceIII PstI \ \ \ 4201 actcaaaagtgcccagctcttcgagcgcgtctgcagcgatcaacccatctgtgatcccat 4260 tgagttttcacgggtcgagaagctcgcgcagacgtcgctagttgggtagacactagggta ^ * ^ * ^ * ^ * ^ * ^ * BsaI \ 4261 tgggagaccaaaagttcatgcggctgctttgaaacaggccactggtgaagctatctacac 4320 accctctggttttcaagtacgccgacgaaactttgtccggtgaccacttcgatagatgtg ^ * ^ * ^ * ^ * ^ * ^ * 4321 agatgacattccccgcatggatggtgaagtttatctggcctttgtccttagtaccaagcc 4380 tctactgtaaggggcgtacctaccacttcaaatagaccggaaacaggaatcatggttcgg ^ * ^ * ^ * ^ * ^ * ^ * PmlI XcmI BspGI \ \ \ 4381 acgtgccaagatcaccaagctggatgccagtgaagctctggccctggacggagtgcatca 4440 tgcacggttctagtggttcgacctacggtcacttcgagaccgggacctgcctcacgtagt ^ * ^ * ^ * ^ * ^ * ^ * BssSI SanDI RcaI \ \ \ 4441 gttcttttgctacaaggacttaacggagcacgagaacgaagtgggacccgtctttcatga 4500 caagaaaacgatgttcctgaattgcctcgtgctcttgcttcaccctgggcagaaagtact ^ * ^ * ^ * ^ * ^ * ^ * BsrDI BpmI NarI \ \ \ 4501 tgagcacgtctttgccgctggagaagtgcattgctatggtcagatagtgggcgccatagc 4560 actcgtgcagaaacggcgacctcttcacgtaacgataccagtctatcacccgcggtatcg ^ * ^ * ^ * ^ * ^ * ^ * BsrBI AceIII \ \ 4561 tgccgataataaggcgttggcccaaagagccgctcggctagtgaaagtggagtacgagga 4620 acggctattattccgcaaccgggtttctcggcgagccgatcactttcacctcatgctcct ^ * ^ * ^ * ^ * ^ * ^ * Bpu1102I BseRI Bce83I SacI \ \ \ \ 4621 gctgagcccggttatcgtgaccatagagcaggccatcgagctcaagtcctatttcccgga 4680 cgactcgggccaatagcactggtatctcgtccggtagctcgagttcaggataaagggcct ^ * ^ * ^ * ^ * ^ * ^ * EciI XcmI BsrDI BseRI \ \ \ \ 4681 ctacccccgattcgtgaccaagggcaatgtggaggaggctttatcccaggcggatcacac 4740 gatgggggctaagcactggttcccgttacacctcctccgaaatagggtccgcctagtgtg ^ * ^ * ^ * ^ * ^ * ^ * EciI BsaI \ \ 4741 tttcgagggcacctgtcgaatgggcggacaggagcacttctatctggagacccatgctgc 4800 aaagctcccgtggacagcttacccgcctgtcctcgtgaagatagacctctgggtacgacg ^ * ^ * ^ * ^ * ^ * ^ * AceIII BpmI BssSI \ \ \ 4801 attggccgtacctcgtgacagcgatgagctggaactcttttgctccacgcagcatccctc 4860 taaccggcatggagcactgtcgctactcgaccttgagaaaacgaggtgcgtcgtagggag ^ * ^ * ^ * ^ * ^ * ^ * BsgI BstAPI \ \ 4861 ggaggtgcagaagctagtggcccatgtaaccgcacttcctgcccaccgtgtcgtctgtcg 4920 cctccacgtcttcgatcaccgggtacattggcgtgaaggacgggtggcacagcagacagc ^ * ^ * ^ * ^ * ^ * ^ * BstXI BspGI \ \ 4921 tgccaagcgtttgggaggcggtttcggcggcaaggagtccagaggcatctccgtggccct 4980 acggttcgcaaaccctccgccaaagccgccgttcctcaggtctccgtagaggcaccggga ^ * ^ * ^ * ^ * ^ * ^ * NruI \ 4981 acccgttgccctggccgcctatcgaatgggtcgtcctgtgcgctgtatgttggatcgcga 5040 tgggcaacgggaccggcggatagcttacccagcaggacacgcgacatacaacctagcgct ^ * ^ * ^ * ^ * ^ * ^ * EarI Bsp24I \ \ 5041 tgaggacatgcttatcaccggcaccaggcatcccttcctcttcaaatacaaagtgggctt 5100 actcctgtacgaatagtggccgtggtccgtagggaaggagaagtttatgtttcacccgaa ^ * ^ * ^ * ^ * ^ * ^ * HgiEII BclI Bsp24I NcoI \ \ \ \ 5101 caccaaggagggtctgatcactgcctgcgacattgagtgctacaacaatgccggttggtc 5160 gtggttcctcccagactagtgacggacgctgtaactcacgatgttgttacggccaaccag ^ * ^ * ^ * ^ * ^ * ^ * NdeI \ 5161 catggatctgtcattttcggtaagagtggtgggtttatggaaaatccatatgtaggtttc 5220 gtacctagacagtaaaagccattctcaccacccaaataccttttaggtatacatccaaag ^ * ^ * ^ * ^ * ^ * ^ * RleAI NsiI BsbI XcmI \ \ \\ 5221 tttatgcataccatgtttgctcaaatatccatgtgttgtattttccattgaacatctggt 5280 aaatacgtatggtacaaacgagtttataggtacacaacataaaaggtaacttgtagacca ^ * ^ * ^ * ^ * ^ * ^ * 5281 tagtgtgggaatttctaggacccctatatgttatcatcctgagtgcaaatagtttgaaac 5340 atcacacccttaaagatcctggggatatacaatagtaggactcacgtttatcaaactttg ^ * ^ * ^ * ^ * ^ * ^ * 5341 ttttaagaatgtttaggtctaatcaggaaagcaagcaccaggattatattgacataaaca 5400 aaaattcttacaaatccagattagtcctttcgttcgtggtcctaatataactgtatttgt ^ * ^ * ^ * ^ * ^ * ^ * 5401 attataaataataatctaaaaattcttaaaaatgatctaatatataaatcctatgtttag 5460 taatatttattattagatttttaagaatttttactagattatatatttaggatacaaatc ^ * ^ * ^ * ^ * ^ * ^ * AclI BssHII Bce83I TaqII XcmI \ \ \ \ \ 5461 gttcttgagcgcgccatgttccactttgagaattgctacaggattcccaacgttcgcgtg 5520 caagaactcgcgcggtacaaggtgaaactcttaacgatgtcctaagggttgcaagcgcac ^ * ^ * ^ * ^ * ^ * ^ * BspMI \ 5521 ggtggatgggtctgcaagacgaacctgccctcgaatacggccttccgtggatttggagga 5580 ccacctacccagacgttctgcttggacgggagcttatgccggaaggcacctaaacctcct ^ * ^ * ^ * ^ * ^ * ^ * SgrAI NruI \ \ 5581 ccacaaggcatgtacgccggtgagcatatcatccgggatgtggcccggatagtgggtcgc 5640 ggtgttccgtacatgcggccactcgtatagtaggccctacaccgggcctatcacccagcg ^ * ^ * ^ * ^ * ^ * ^ * BpmI \ 5641 gatgtggtggatgtgatgcggctgaacttctacaagactggagactacacacactaccac 5700 ctacaccacctacactacgccgacttgaagatgttctgacctctgatgtgtgtgatggtg ^ * ^ * ^ * ^ * ^ * ^ * PvuII AlwNI BsrBI PflMI BpmI BpmI \ \\ \ 5701 cagcagctggagcacttccccatcgagcggtgtctggaggattgcttgaagcagtcgaga 5760 gtcgtcgacctcgtgaaggggtagctcgccacagacctcctaacgaacttcgtcagctct ^ * ^ * ^ * ^ * ^ * ^ * EciI \ 5761 tacgacgagaagcggcaggatattgctcgattcaatcgggagaatcgctggcggaaacgc 5820 atgctgctcttcgccgtcctataacgagctaagttagccctcttagcgaccgcctttgcg ^ * ^ * ^ * ^ * ^ * ^ * DrdII \ 5821 ggcatggcggtggtgcccaccaagtatggaatcgcatttggagtgatgcacttgaaccaa 5880 ccgtaccgccaccacgggtggttcataccttagcgtaaacctcactacgtgaacttggtt ^ * ^ * ^ * ^ * ^ * ^ * BclI BsaBI BamHI BsbI \ \ \ \ 5881 gcgggatcgctgatcaacatctatggtgatggatccgtgttgctttcgcacggaggagtt 5940 cgccctagcgactagttgtagataccactacctaggcacaacgaaagcgtgcctcctcaa ^ * ^ * ^ * ^ * ^ * ^ * Tth111I BseRI BglI BstXI \ \ \ \ 5941 gagatcggacaaggtctgaataccaagatgattcagtgcgccgccagggctctggggatt 6000 ctctagcctgttccagacttatggttctactaagtcacgcggcggtcccgagacccctaa ^ * ^ * ^ * ^ * ^ * ^ * XmnI BsbI RleAI \ \ \ 6001 ccttcggaactgattcacatttcggaaacggccacggataaagtacccaacacttcaccc 6060 ggaagccttgactaagtgtaaagcctttgccggtgcctatttcatgggttgtgaagtggg ^ * ^ * ^ * ^ * ^ * ^ * BamHI TaqII \ \ 6061 acggcggcgagtgtgggatccgatctgaacggaatggccgtactggatgcgtgtgaaaag 6120 tgccgccgctcacaccctaggctagacttgccttaccggcatgacctacgcacacttttc ^ * ^ * ^ * ^ * ^ * ^ * NarI BsrDI BpmI \ \ \ 6121 ttgaacaaaagactggcgcccatcaaggaggcattgcctggaggcacctggaaggagtgg 6180 aacttgttttctgaccgcgggtagttcctccgtaacggacctccgtggaccttcctcacc ^ * ^ * ^ * ^ * ^ * ^ * PvuI SmaI \ \ 6181 atcaacaaggcgtatttcgatcgggtcagcctctcggccacaggattctatgccatgccc 6240 tagttgttccgcataaagctagcccagtcggagagccggtgtcctaagatacggtacggg ^ * ^ * ^ * ^ * ^ * ^ * EcoRV \ 6241 gggattggatatcacccggaaacgaatcccaatgctcgcacctatagctactacacgaat 6300 ccctaacctatagtgggcctttgcttagggttacgagcgtggatatcgatgatgtgctta ^ * ^ * ^ * ^ * ^ * ^ * BsaXI ClaI BglI BcgI Bpu1102I \ \ \ \ \ 6301 ggcgtgggagtcactgtggtagagatcgattgcctgactggcgaccatcaggtgctcagc 6360 ccgcaccctcagtgacaccatctctagctaacggactgaccgctggtagtccacgagtcg ^ * ^ * ^ * ^ * ^ * ^ * BsaBI BcgI PshAI \ \ \ 6361 acagacatcgtgatggacatcggctctagcctgaatccggctattgacattggtcagatc 6420 tgtctgtagcactacctgtagccgagatcggacttaggccgataactgtaaccagtctag ^ * ^ * ^ * ^ * ^ * ^ * BsmI BpmI \ \ 6421 gagggagcattcatgcagggctatggactgttcactttggaggaactcatgtactcacca 6480 ctccctcgtaagtacgtcccgatacctgacaagtgaaacctccttgagtacatgagtggt ^ * ^ * ^ * ^ * ^ * ^ * SphI BsrGI \ \ 6481 caaggcatgctttactccagaggtccgggcatgtacaagctgccaggatttgccgacatt 6540 gttccgtacgaaatgaggtctccaggcccgtacatgttcgacggtcctaaacggctgtaa ^ * ^ * ^ * ^ * ^ * ^ * Bsp24I SmaI PinAI TaqII \ \ \ \ 6541 cccggggagttcaatgtcagcctactgaccggtgcccccaatccacgggcagtctactct 6600 gggcccctcaagttacagtcggatgactggccacgggggttaggtgcccgtcagatgaga ^ * ^ * ^ * ^ * ^ * ^ * EarI BstXI EarI Bsp24I BsrBI SapI BsmI BstAPI \ \ \ \ \ \ \ 6601 tccaaggcagtgggtgaacctccgctcttcattggatcatctgcattctttgccattaag 6660 aggttccgtcacccacttggaggcgagaagtaacctagtagacgtaagaaacggtaattc ^ * ^ * ^ * ^ * ^ * ^ * NarI BsrDI PvuII NruI Bce83I \ \ \ \ \ 6661 gaggccattgcagctgctcgcgaggatcagggcttgagtggtgacttcccactggaggcg 6720 ctccggtaacgtcgacgagcgctcctagtcccgaactcaccactgaagggtgacctccgc ^ * ^ * ^ * ^ * ^ * ^ * BsmI BpmI NspV \ \ \ 6721 ccttccacatcggcacgcattcgaattgcttgtcaggataagttcacggaactggtaagt 6780 ggaaggtgtagccgtgcgtaagcttaacgaacagtcctattcaagtgccttgaccattca ^ * ^ * ^ * ^ * ^ * ^ * VspI VspI \ \ 6781 tacccttggattagtttagaacattaaactaacttttattattaatttattaattatagc 6840 atgggaacctaatcaaatcttgtaatttgattgaaaataataattaaataattaatatcg ^ * ^ * ^ * ^ * ^ * ^ * DrdII NcoI \ \ 6841 ttgaaatacccgaaccaggatcatttacgccatggaacattgtgccttaaaattgttttt 6900 aactttatgggcttggtcctagtaaatgcggtaccttgtaacacggaattttaacaaaaa ^ * ^ * ^ * ^ * ^ * ^ * AflII DraI \ \ 6901 attgtttgtactgcttaagcatttaaaatgacgattttatttgttaatttgtttatatat 6960 taacaaacatgacgaattcgtaaattttactgctaaaataaacaattaaacaaatatata ^ * ^ * ^ * ^ * ^ * ^ * DraI AceIII \ \ 6961 acatagtgaattaaatgttttaaaaataattgagtcgtttaatgtgtaaactaagctgga 7020 tgtatcacttaatttacaaaatttttattaactcagcaaattacacatttgattcgacct ^ * ^ * ^ * ^ * ^ * ^ * SspI BpmI VspI SspI \ \ \ \ 7021 gagctgtttgaacaaatttatataccactgattaatattaaatattccttttagattaag 7080 ctcgacaaacttgtttaaatatatggtgactaattataatttataaggaaaatctaattc ^ * ^ * ^ * ^ * ^ * ^ * SphI BssSI BstAPI \ \ \ 7081 aaaaattcaatggttacttttttgttctatctattattcaatagaatctcgtggcatgca 7140 tttttaagttaccaatgaaaaaacaagatagataataagttatcttagagcaccgtacgt ^ * ^ * ^ * ^ * ^ * ^ * BsmI NsiI BsrDI MunI VspI \ \ \ \ 7141 atgcattctgattttcaattgaaaagtcttagtcactatttattttgagtatattaatga 7200 tacgtaagactaaaagttaacttttcagaatcagtgataaataaaactcatataattact ^ * ^ * ^ * ^ * ^ * ^ * 7201 aagttgagcaagttttccgatgaattgaaatatgagttccgatttcggcgcgactgctac 7260 ttcaactcgttcaaaaggctacttaactttatactcaaggctaaagccgcgctgacgatg ^ * ^ * ^ * ^ * ^ * ^ * HindIII \ 7261 ccgaaaatataagctcaatcaaaagcaagctt 7320 ggcttttatattcgagttagttttcgttcgaa ^ * ^ * ^ * ^ * ^ * ^ *