module name: |
rosy module |
size: |
7292 bp |
cloning sites: |
HindIII / HindIII |
creation: |
7.2kb HindIII rosy fragment from pP{Car20} |
Ladder Map of Enzyme Sites:
# hits
700 1400 2100 2800 3500 4200 4900 5600 6300 7000
: : : : : : : : : :
AflII ------------------------------------------------\--- 1
AhdI -------------------------/-------------------------- 1
AlwNI ----------------------------------------/----------- 1
BbsI --------------------------\------------------------- 1
: : : : : : : : : :
BciVI --/------------------------------------------------- 1
BplI --------------------|------------------------------- 1
BspLU11I ---------\------------------------------------------ 1
BsrGI ----------------------------------------------\----- 1
: : : : : : : : : :
BssHII --------------------------------------\------------- 1
BstZ17I -----|---------------------------------------------- 1
DrdI -----------------/---------------------------------- 1
EcoNI --\------------------------------------------------- 1
: : : : : : : : : :
EcoRI ---------------------\------------------------------ 1
EcoRV --------------------------------------------|------- 1
FspI ---------------|------------------------------------ 1
HgiEII -----------------------------------|---------------- 1
: : : : : : : : : :
MluI -------------------\-------------------------------- 1
NdeI ------------------------------------\--------------- 1
NgoAIV ------------------------\--------------------------- 1
NspV -----------------------------------------------\---- 1
700 1400 2100 2800 3500 4200 4900 5600 6300 7000
: : : : : : : : : :
Pfl1108I ---|------------------------------------------------ 1
PinAI ----------------------------------------------\----- 1
PmeI ----------------|----------------------------------- 1
PshAI ---------------------------------------------|------ 1
: : : : : : : : : :
PvuI -------------------------------------------/-------- 1
SanDI -------------------------------\-------------------- 1
SbfI -/-------------------------------------------------- 1
ScaI -----------------------------|---------------------- 1
: : : : : : : : : :
SexAI ----------------------\----------------------------- 1
SfiI ----------------------------/----------------------- 1
SgrAI ---------------------------------------\------------ 1
SnaBI -----|---------------------------------------------- 1
: : : : : : : : : :
StuI ----------------------------|----------------------- 1
Tth111I ------------------------------------------\--------- 1
UbaDI ----------------------|----------------------------- 1
XhoI --------------------\------------------------------- 1
: : : : : : : : : :
XmnI ------------------------------------------|--------- 1
AclI ---------------------------\----------\------------- 2
BcgI ------------------------/--------------------/------ 2
BcgI -----------------------/--------------------/------- 2
700 1400 2100 2800 3500 4200 4900 5600 6300 7000
: : : : : : : : : :
BfiI -------------------\------\------------------------- 2
Bpu10I --------------\-\----------------------------------- 2
BsaXI ----------------------------|---------------|------- 2
BsmBI -\--------\----------------------------------------- 2
: : : : : : : : : :
BspEI ------------------\-------\------------------------- 2
BspGI -------------------------------|--|----------------- 2
DraIII -------/------------------/------------------------- 2
HindIII \--------------------------------------------------\ 2
: : : : : : : : : :
NcoI ------------------------------------\-----------\--- 2
NsiI ------------------------------------/-------------/- 2
PmlI |-----------------------------|--------------------- 2
PstI -/---------------------------/---------------------- 2
: : : : : : : : : :
RcaI -------------------------\-----\-------------------- 2
SmaI --------------------------------------------|-|----- 2
SphI ---------------------------------------------/----/- 2
BglI ----------------------------/-------------/-/------- 3
: : : : : : : : : :
Bpu1102I ----------------------------\---\-----------\------- 3
ClaI ------------\-----------\-------------------\------- 3
RleAI --------------/----------------------/----/--------- 3
SapI ---------------------\-------\----------------\----- 3
700 1400 2100 2800 3500 4200 4900 5600 6300 7000
: : : : : : : : : :
SspI ------|------------------------------------------2-- 3
TaqII ---------/--------/--/------------------------------ 3
ApaLI -------------\----\-----\---\----------------------- 4
BamHI -------------------\-\-------------------\\--------- 4
: : : : : : : : : :
BclI --------2---------------------------\----\---------- 4
BglII --\---\----------\---------\------------------------ 4
Bsp24I /------------------/---------------/----------/----- 4
Bsp24I /------------------/----------------/---------/----- 4
: : : : : : : : : :
BsrBI ----------------------------|---|-------|-----|----- 4
BstEII -----------------\-\\-\----------------------------- 4
DrdII ----|------------------|-----------------|------|--- 4
MscI ---------|-----|---------|--|----------------------- 4
: : : : : : : : : :
NarI ------------------\-------------\----------\---\---- 4
NruI |----------------------------------|---|-------|---- 4
PvuII ----------------------2-----------------|------|---- 4
SacI --------------/----------/--/---/------------------- 4
: : : : : : : : : :
BsaI ---------\-----------\--\----\---\------------------ 5
BspMI -3-------\-----------------------------\------------ 5
BsrDI -------------------------------/-/---------/---/--/- 5
BstAPI -/-----------------/--------------/------------/--/- 5
700 1400 2100 2800 3500 4200 4900 5600 6300 7000
: : : : : : : : : :
DraI -------|--------2-------------------------------||-- 5
EarI ---------------------\-------\-----\----------2----- 5
Bce83I ------/-2-----------------------/-----/--------/---- 6
BsbI --|-------|----|---------------------|---||--------- 6
: : : : : : : : : :
BsgI ---------/--------/2/-------------/----------------- 6
BsmI ------------/-------/------------------------///--/- 6
BssSI ---------------\----------\-\--\-\----------------\- 6
EciI ------------------|--------||----2-------|---------- 6
: : : : : : : : : :
MunI -\-----------2-\\---------------------------------\- 6
PflMI --------------/--/---/-----2------------/----------- 6
VspI ------\----------\------------------------------2\\- 6
BsaBI |-------|-------|-------||---------------|---|------ 7
: : : : : : : : : :
BstXI /---------------------/--/-/------/-------/---/----- 7
Eco57I --/-----2-------------2/-----/---------------------- 7
XcmI ---/-------------------/---/---/-/---//------------- 7
BseRI ----------/-------/---/----/-/--//-------/---------- 8
: : : : : : : : : :
TaqII /-/-----------/--------//-------------/---/---/----- 8
AceIII --------------\-------\\\---2\--\\---------------\-- 10
BpmI ----------------------//-/-----/-/------3--/-/-/-/-- 12
Linear Map of Sequence:
HindIII Bsp24I
\ \
1 aagctttaactatgtttaatctcccatagagcttcgttatcttcggacgcgatgtggcgc 60
ttcgaaattgatacaaattagagggtatctcgaagcaatagaagcctgcgctacaccgcg
^ * ^ * ^ * ^ * ^ * ^ *
Bsp24I BsaBI
\ \
61 agatcccgtgctttcgtaacagttttctctacggaatcagcggagggatcggcatcggct 120
tctagggcacgaaagcattgtcaaaagagatgccttagtcgcctccctagccgtagccga
^ * ^ * ^ * ^ * ^ * ^ *
PmlI
NruI BstXI TaqII
\ \ \
121 tgctgacttttctgggcacctcgcgaacccacctgtccacccacgtgggcttcggctcct 180
acgactgaaaagacccgtggagcgcttgggtggacaggtgggtgcacccgaagccgagga
^ * ^ * ^ * ^ * ^ * ^ *
PstI
SbfI
BspMI BspMI
\ \ \
181 tcttctgcggcaccatcgcctactggatgacctgcaggtaagtgactgtgattgaaaccg 240
agaagacgccgtggtagcggatgacctactggacgtccattcactgacactaactttggc
^ * ^ * ^ * ^ * ^ * ^ *
BspMI
\
241 tgcgtcgcccttatcacttaattatcaatgtggcatccgcaggtatcaatggtccgtcag 300
acgcagcgggaatagtgaattaatagttacaccgtaggcgtccatagttaccaggcagtc
^ * ^ * ^ * ^ * ^ * ^ *
MunI
BstAPI BsmBI EcoNI
\ \ \ \
301 gagattcgagcagcagcaattgcgtgaggcgatgagacgacaagccctttatgagggcac 360
ctctaagctcgtcgtcgttaacgcactccgctactctgctgttcgggaaatactcccgtg
^ * ^ * ^ * ^ * ^ * ^ *
BglII
TaqII Eco57I
\ \ \
361 ccaaagggagagagacttagatctgaagtcggcgtagcataagtcagatccttcattact 420
ggtttccctctctctgaatctagacttcagccgcatcgtattcagtctaggaagtaatga
^ * ^ * ^ * ^ * ^ * ^ *
BciVI
\
421 acttcgatgtttgttttcactctaggataccaagtaatcctggcattgtcaaatcccttg 480
tgaagctacaaacaaaagtgagatcctatggttcattaggaccgtaacagtttagggaac
^ * ^ * ^ * ^ * ^ * ^ *
BsbI
\
481 tgttgtaaactaccttttgttaccaaactttatagtagccaatatattatatatatgttg 540
acaacatttgatggaaaacaatggtttgaaatatcatcggttatataatatatatacaac
^ * ^ * ^ * ^ * ^ * ^ *
Pfl1108I
\
541 taagtgaaatcatagcttggagtttgcttgccactcgtagggaattagttatgccaccat 600
attcactttagtatcgaacctcaaacgaacggtgagcatcccttaatcaatacggtggta
^ * ^ * ^ * ^ * ^ * ^ *
XcmI DrdII
\ \
601 ctttgatttgggattgacagaaatcaatccatcattttaatgattgtggttcctaaaacc 660
gaaactaaaccctaactgtctttagttaggtagtaaaattactaacaccaaggattttgg
^ * ^ * ^ * ^ * ^ * ^ *
661 tgttgggctttattgtgctttcgatttgatttaagctgatagagctagttgtttactttt 720
acaacccgaaataacacgaaagctaaactaaattcgactatctcgatcaacaaatgaaaa
^ * ^ * ^ * ^ * ^ * ^ *
721 gaagaacattgaacccattttgacgaccttttaactttttaatagtttttttttaccatt 780
cttcttgtaacttgggtaaaactgctggaaaattgaaaaattatcaaaaaaaaatggtaa
^ * ^ * ^ * ^ * ^ * ^ *
SnaBI
\
781 tacataaaagcaaaatttacagtctaaaagttgtatattcaatacgttctggtacgtaaa 840
atgtattttcgttttaaatgtcagattttcaacatataagttatgcaagaccatgcattt
^ * ^ * ^ * ^ * ^ * ^ *
BstZ17I
\
841 agggttatttaatgcaccatttttacaactttagatagtatactttttacttgagtggga 900
tcccaataaattacgtggtaaaaatgttgaaatctatcatatgaaaaatgaactcaccct
^ * ^ * ^ * ^ * ^ * ^ *
Bce83I VspI
\ \
901 gccatatcacaaaaatgaaatgaaaggttaaaaaagtatctaaaagtaagtttcaattaa 960
cggtatagtgtttttactttactttccaattttttcatagattttcattcaaagttaatt
^ * ^ * ^ * ^ * ^ * ^ *
SspI
\
961 tattctgaaaaataattgcatttcatgtacgtttctctgaaatacccctaattcgatact 1020
ataagactttttattaacgtaaagtacatgcaaagagactttatggggattaagctatga
^ * ^ * ^ * ^ * ^ * ^ *
BglII
\
1021 atttatagatctggaagatatatagctaggaaatagaattatatatttgcctagtttgaa 1080
taaatatctagaccttctatatatcgatcctttatcttaatatataaacggatcaaactt
^ * ^ * ^ * ^ * ^ * ^ *
DraIII DraI
\ \
1081 caccttgtgaacattttgtttaaaaaaaaagcgtatggtggtactagaaaactttaagat 1140
gtggaacacttgtaaaacaaatttttttttcgcataccaccatgatcttttgaaattcta
^ * ^ * ^ * ^ * ^ * ^ *
1141 acagataaataacttcactataccgaggttttggtgtttcttgagactgaaggacaatct 1200
tgtctatttattgaagtgatatggctccaaaaccacaaagaactctgacttcctgttaga
^ * ^ * ^ * ^ * ^ * ^ *
BclI
Bce83I Eco57I Bce83I Eco57I
\ \ \ \\
1201 tcaaaatggtcttaattcttgagatagaaaagctgaagttctcatgctgaatagtgatca 1260
agttttaccagaattaagaactctatcttttcgacttcaagagtacgacttatcactagt
^ * ^ * ^ * ^ * ^ * ^ *
BclI BsaBI
\ \
1261 gtacgattttatctgacagattgctttcctagctgatcataccgatctcggatgggtatc 1320
catgctaaaatagactgtctaacgaaaggatcgactagtatggctagagcctacccatag
^ * ^ * ^ * ^ * ^ * ^ *
BspMI MscI
\ \
1321 tgctctaatccgagatagcaggtagtagaactgctgcctcgtaacccctagacatggcca 1380
acgagattaggctctatcgtccatcatcttgacgacggagcattggggatctgtaccggt
^ * ^ * ^ * ^ * ^ * ^ *
BsgI
BspLU11I TaqII BsaI
\ \ \ \
1381 tttccacgcacatgtaggcgatcctgcactcgtcggtctctcgtcttatctcacaaaaat 1440
aaaggtgcgtgtacatccgctaggacgtgagcagccagagagcagaatagagtgttttta
^ * ^ * ^ * ^ * ^ * ^ *
1441 aaaagtcggcgtttggatggattgctcggctggctgttggcacattcattacgttttcgt 1500
ttttcagccgcaaacctacctaacgagccgaccgacaaccgtgtaagtaatgcaaaagca
^ * ^ * ^ * ^ * ^ * ^ *
BsmBI BsbI
\ \
1501 tgacggcgcgagacggttgtgttgttttttaatcggcgggcacatgaggttgtcggttct 1560
actgccgcgctctgccaacacaacaaaaaattagccgcccgtgtactccaacagccaaga
^ * ^ * ^ * ^ * ^ * ^ *
BseRI
\
1561 ctaattttgaaataaaaaacttgttttcgctttacattcaaggaacgcacctcctcccct 1620
gattaaaactttattttttgaacaaaagcgaaatgtaagttccttgcgtggaggagggga
^ * ^ * ^ * ^ * ^ * ^ *
1621 ccatatatgtaaacgagcttcgtaattgcaggctaacacttatataggcattggtactga 1680
ggtatatacatttgctcgaagcattaacgtccgattgtgaatatatccgtaaccatgact
^ * ^ * ^ * ^ * ^ * ^ *
1681 gatacagcgagataccgtgagctacagatacatagcacttcacgatgtctaactcggttt 1740
ctatgtcgctctatggcactcgatgtctatgtatcgtgaagtgctacagattgagccaaa
^ * ^ * ^ * ^ * ^ * ^ *
1741 tggtgtttttcgtgaatggaaagaaggtacttaaagatactcggatgccttgcgtgtctt 1800
accacaaaaagcacttacctttcttccatgaatttctatgagcctacggaacgcacagaa
^ * ^ * ^ * ^ * ^ * ^ *
ClaI
\
1801 tccttaaagtcggcccactcttactatatcgattccctttattgttttattgccctaatt 1860
aggaatttcagccgggtgagaatgatatagctaagggaaataacaaaataacgggattaa
^ * ^ * ^ * ^ * ^ * ^ *
BsmI MunI
\ \
1861 gcgtttaactggcattcgatggaatttgccttcttgctcaattgaatgtgtcgaatgtat 1920
cgcaaattgaccgtaagctaccttaaacggaagaacgagttaacttacacagcttacata
^ * ^ * ^ * ^ * ^ * ^ *
MunI
\
1921 atagtatttgctgtgcatatagtgataacaattgacccccatgtgaagcctcgttgtttg 1980
tatcataaacgacacgtatatcactattgttaactgggggtacacttcggagcaacaaac
^ * ^ * ^ * ^ * ^ * ^ *
ApaLI RleAI
\ \
1981 ctggtggccccttatcgcccgatcaagatggtgcacttgccaatcgcgcatgggagcagt 2040
gaccaccggggaatagcgggctagttctaccacgtgaacggttagcgcgtaccctcgtca
^ * ^ * ^ * ^ * ^ * ^ *
TaqII AceIII Bpu10I
\ \ \
2041 gctactcgaatgtgggtgagctgggatcgtcatgggcatttgtgtctgccagatagctca 2100
cgatgagcttacacccactcgaccctagcagtacccgtaaacacagacggtctatcgagt
^ * ^ * ^ * ^ * ^ * ^ *
PflMI SacI
\ \
2101 ggccctctaatccgcacattcccaccggctgggcggtgtttacaatccagtgagctcggc 2160
ccgggagattaggcgtgtaagggtggccgacccgccacaaatgttaggtcactcgagccg
^ * ^ * ^ * ^ * ^ * ^ *
MscI BsbI MunI
\ \ \
2161 tggattattcagataaagctggccatatttagcatctggtgttggtggagtgcaattgga 2220
acctaataagtctatttcgaccggtataaatcgtagaccacaaccacctcacgttaacct
^ * ^ * ^ * ^ * ^ * ^ *
FspI
\
2221 gttctttactttgtgctcacagcttgcgcacaagtgaagtaaataataataaataaccag 2280
caagaaatgaaacacgagtgtcgaacgcgtgttcacttcatttattattatttattggtc
^ * ^ * ^ * ^ * ^ * ^ *
BssSI BsaBI Bpu10I
\ \ \
2281 ttgctcgtgcgcccggcatttgatagtgatagatagcaatctctgtttgctcagggctgg 2340
aacgagcacgcgggccgtaaactatcactatctatcgttagagacaaacgagtcccgacc
^ * ^ * ^ * ^ * ^ * ^ *
DraI
PmeI MunI
\ \
2341 cagagcagataacactttcgctgttttaagtttaaacaaagtcacagttttgacaaacaa 2400
gtctcgtctattgtgaaagcgacaaaattcaaatttgtttcagtgtcaaaactgtttgtt
^ * ^ * ^ * ^ * ^ * ^ *
DraI BglII
\ \
2401 ttgctttaaaccatataaacttataattaaagcaattataatgggttaagtaagcacaga 2460
aacgaaatttggtatatttgaatattaatttcgttaatattacccaattcattcgtgtct
^ * ^ * ^ * ^ * ^ * ^ *
VspI
PflMI
\ \
2461 tctagctacaagatacaagatacaaattttccaattaatggataaatggcattagatact 2520
agatcgatgttctatgttctatgtttaaaaggttaattacctatttaccgtaatctatga
^ * ^ * ^ * ^ * ^ * ^ *
BstEII
\
2521 tttgggaaattgatttcgtttttttcattttattatatttatacatttcttattccaggt 2580
aaaccctttaactaaagcaaaaaaagtaaaataatataaatatgtaaagaataaggtcca
^ * ^ * ^ * ^ * ^ * ^ *
BseRI
BspEI
DrdI TaqII ApaLI
\ \ \ \ \
2581 gaccgaagtgtcgcctgatccggagtgcacgctcctcacattcctgcgcgaaaagctgcg 2640
ctggcttcacagcggactaggcctcacgtgcgaggagtgtaaggacgcgcttttcgacgc
^ * ^ * ^ * ^ * ^ * ^ *
BsgI EciI NarI
\ \ \
2641 gctgtgcggaacgaagttgggatgtgcggaaggcggatgcggcgcctgcaccgtgatggt 2700
cgacacgccttgcttcaaccctacacgccttccgcctacgccgcggacgtggcactacca
^ * ^ * ^ * ^ * ^ * ^ *
2701 gtcccgcctagaccgtcgggccaacaagatacgtcacctggcggtcaacgcctgcctgac 2760
cagggcggatctggcagcccggttgttctatgcagtggaccgccagttgcggacggactg
^ * ^ * ^ * ^ * ^ * ^ *
BstEII Bsp24I
\ \
2761 gccagtgtgctcgatgcacggatgtgcggtgaccactgtggagggtatagggagcaccaa 2820
cggtcacacgagctacgtgcctacacgccactggtgacacctcccatatccctcgtggtt
^ * ^ * ^ * ^ * ^ * ^ *
BsgI
BstAPI BfiI
MluI Bsp24I BsgI BamHI
\ \ \ \ \\
2821 aacgcgtctgcatccggtgcaggagcgactgcccaaggcgcacggatcccagtgcggctt 2880
ttgcgcagacgtaggccacgtcctcgctgacgggttccgcgtgcctagggtcacgccgaa
^ * ^ * ^ * ^ * ^ * ^ *
BplI
\
2881 ctgcacgccgggcattgtgatgtccatgtacgcacttctgcggaacgcggagcaaccctc 2940
gacgtgcggcccgtaacactacaggtacatgcgtgaagacgccttgcgcctcgttgggag
^ * ^ * ^ * ^ * ^ * ^ *
BsgI
BsmI BstEII
\ \ \
2941 tatgcgagacttggaggtggcattccaaggtaacctgtgccgctgcaccggctatcgacc 3000
atacgctctgaacctccaccgtaaggttccattggacacggcgacgtggccgatagctgg
^ * ^ * ^ * ^ * ^ * ^ *
XhoI
\
3001 cattctcgagggctacaagacgttcaccaaggagtttgcctgcggaatgggcgagaagtg 3060
gtaagagctcccgatgttctgcaagtggttcctcaaacggacgccttacccgctcttcac
^ * ^ * ^ * ^ * ^ * ^ *
EarI
SapI
BsaI TaqII
\ \ \
3061 ctgcaaagttagtgggaaaggatgtggaaccgatgcggagaccgatgacaagctcttcga 3120
gacgtttcaatcaccctttcctacaccttggctacgcctctggctactgttcgagaagct
^ * ^ * ^ * ^ * ^ * ^ *
BamHI Eco57I
EcoRI PflMI UbaDI
\ \ \ \\
3121 gcgcagcgaattccagcccttggatcccagccaggaacccatcttcccaccggaacttca 3180
cgcgtcgcttaaggtcgggaacctagggtcggtccttgggtagaagggtggccttgaagt
^ * ^ * ^ * ^ * ^ * ^ *
SexAI
PvuII BstEII
\ \ \
3181 gctgagtgacgccttcgattcgcagagtttgatctttagttcggatagggtgacctggta 3240
cgactcactgcggaagctaagcgtctcaaactagaaatcaagcctatcccactggaccat
^ * ^ * ^ * ^ * ^ * ^ *
Eco57I PvuII
AceIII BseRI
BstXI BpmI Eco57I
\ \\ \\ \ \
3241 tcgtcccaccaatctggaggagctgcttcagctgaaggccaaacatccggctgccaagct 3300
agcagggtggttagacctcctcgacgaagtcgacttccggtttgtaggccgacggttcga
^ * ^ * ^ * ^ * ^ * ^ *
XcmI
\
3301 ggtcgtgggcaatacggaagtgggcgttgaggttaagttcaagcacttcctctacccgca 3360
ccagcacccgttatgccttcacccgcaactccaattcaagttcgtgaaggagatgggcgt
^ * ^ * ^ * ^ * ^ * ^ *
DrdII
AceIII TaqII BcgI BpmI
\ \ \ \ \
3361 cctcatcaatcccacccaggtgaaggagctgctggagatcaaagagaaccaggatggcat 3420
ggagtagttagggtgggtccacttcctcgacgacctctagtttctcttggtcctaccgta
^ * ^ * ^ * ^ * ^ * ^ *
ClaI
BcgI BsaBI AceIII
\ \ \ \
3421 ttacttcggtgcggctgtcagtttgatggagatcgatgcgcttctgcggcagagaatcga 3480
aatgaagccacgccgacagtcaaactacctctagctacgcgaagacgccgtctcttagct
^ * ^ * ^ * ^ * ^ * ^ *
BsaI ApaLI
\ \
3481 gctgctgccggaatcggagaccagattgttccagtgcaccgtggatatgcttcactactt 3540
cgacgacggccttagcctctggtctaacaaggtcacgtggcacctatacgaagtgatgaa
^ * ^ * ^ * ^ * ^ * ^ *
NgoAIV TaqII RcaI AhdI
\ \ \ \
3541 tgccggcaagcagatccgcaacgtcgcctgtttgggtggaaacatcatgaccggcagtcc 3600
acggccgttcgtctaggcgttgcagcggacaaacccacctttgtagtactggccgtcagg
^ * ^ * ^ * ^ * ^ * ^ *
BsaBI SacI MscI
\ \ \
3601 catttccgatatgaatcctgtgctctcggcagcaggagctcaactggaggtggccagttt 3660
gtaaaggctatacttaggacacgagagccgtcgtcctcgagttgacctccaccggtcaaa
^ * ^ * ^ * ^ * ^ * ^ *
BstXI
BpmI BfiI
\ \ \
3661 tgtggatggaaagctccaaaagagatcagttcacatgggaactgggttcttcactggcta 3720
acacctacctttcgaggttttctctagtcaagtgtacccttgacccaagaagtgaccgat
^ * ^ * ^ * ^ * ^ * ^ *
DraIII
BssSI BbsI
\ \ \
3721 tcgcaggaatgttatcgaagcccacgaggtgctgctgggcatccactttcggaagaccac 3780
agcgtccttacaatagcttcgggtgctccacgacgacccgtaggtgaaagccttctggtg
^ * ^ * ^ * ^ * ^ * ^ *
BspEI
\
3781 tccggaccagtatatcgttgcttttaagcaggccagaagaagggatgatgacatagccat 3840
aggcctggtcatatagcaacgaaaattcgtccggtcttcttccctactactgtatcggta
^ * ^ * ^ * ^ * ^ * ^ *
EciI
AclI BstXI BglII
\ \ \ \
3841 cgtaaatgccgcaataaacgttcgctttgaggaaaaatccaacattgtggcggagatctc 3900
gcatttacggcgttatttgcaagcgaaactcctttttaggttgtaacaccgcctctagag
^ * ^ * ^ * ^ * ^ * ^ *
XcmI
BseRI PflMI
\ \\
3901 gatggcttttggtggaatggcaccaaccacagtgctcgctcctcgaacttcccaactgat 3960
ctaccgaaaaccaccttaccgtggttggtgtcacgagcgaggagcttgaagggttgacta
^ * ^ * ^ * ^ * ^ * ^ *
AceIII
BssSI ApaLI
PflMI AceIII EciI BsaXI
\ \ \ \ \ \\
3961 ggttgggcaggagtggagccaccagctcgtggagcgcgtggcggagagcttgtgcacgga 4020
ccaacccgtcctcacctcggtggtcgagcacctcgcgcaccgcctctcgaacacgtgcct
^ * ^ * ^ * ^ * ^ * ^ *
BsrBI SacI
\ \
4021 gctgcctttggctgcctccgctccgggtggcatgatcgcctatcgtcgagctctggtggt 4080
cgacggaaaccgacggaggcgaggcccaccgtactagcggatagcagctcgagaccacca
^ * ^ * ^ * ^ * ^ * ^ *
MscI
BglI
StuI SfiI Bpu1102I Eco57I
\ \ \ \ \
4081 gagcctgttcttcaaggcctatctggccatttccctgaagctgagcaagtcagggatcac 4140
ctcggacaagaagttccggatagaccggtaaagggacttcgactcgttcagtccctagtg
^ * ^ * ^ * ^ * ^ * ^ *
BseRI ScaI
\ \
4141 atcttccgatgctctgccaccggaggagcgaagtggtgccgagacattccacacaccagt 4200
tagaaggctacgagacggtggcctcctcgcttcaccacggctctgtaaggtgtgtggtca
^ * ^ * ^ * ^ * ^ * ^ *
EarI
SapI
AceIII PstI
\ \ \
4201 actcaaaagtgcccagctcttcgagcgcgtctgcagcgatcaacccatctgtgatcccat 4260
tgagttttcacgggtcgagaagctcgcgcagacgtcgctagttgggtagacactagggta
^ * ^ * ^ * ^ * ^ * ^ *
BsaI
\
4261 tgggagaccaaaagttcatgcggctgctttgaaacaggccactggtgaagctatctacac 4320
accctctggttttcaagtacgccgacgaaactttgtccggtgaccacttcgatagatgtg
^ * ^ * ^ * ^ * ^ * ^ *
4321 agatgacattccccgcatggatggtgaagtttatctggcctttgtccttagtaccaagcc 4380
tctactgtaaggggcgtacctaccacttcaaatagaccggaaacaggaatcatggttcgg
^ * ^ * ^ * ^ * ^ * ^ *
PmlI XcmI BspGI
\ \ \
4381 acgtgccaagatcaccaagctggatgccagtgaagctctggccctggacggagtgcatca 4440
tgcacggttctagtggttcgacctacggtcacttcgagaccgggacctgcctcacgtagt
^ * ^ * ^ * ^ * ^ * ^ *
BssSI SanDI RcaI
\ \ \
4441 gttcttttgctacaaggacttaacggagcacgagaacgaagtgggacccgtctttcatga 4500
caagaaaacgatgttcctgaattgcctcgtgctcttgcttcaccctgggcagaaagtact
^ * ^ * ^ * ^ * ^ * ^ *
BsrDI BpmI NarI
\ \ \
4501 tgagcacgtctttgccgctggagaagtgcattgctatggtcagatagtgggcgccatagc 4560
actcgtgcagaaacggcgacctcttcacgtaacgataccagtctatcacccgcggtatcg
^ * ^ * ^ * ^ * ^ * ^ *
BsrBI AceIII
\ \
4561 tgccgataataaggcgttggcccaaagagccgctcggctagtgaaagtggagtacgagga 4620
acggctattattccgcaaccgggtttctcggcgagccgatcactttcacctcatgctcct
^ * ^ * ^ * ^ * ^ * ^ *
Bpu1102I BseRI Bce83I SacI
\ \ \ \
4621 gctgagcccggttatcgtgaccatagagcaggccatcgagctcaagtcctatttcccgga 4680
cgactcgggccaatagcactggtatctcgtccggtagctcgagttcaggataaagggcct
^ * ^ * ^ * ^ * ^ * ^ *
EciI
XcmI BsrDI BseRI
\ \ \ \
4681 ctacccccgattcgtgaccaagggcaatgtggaggaggctttatcccaggcggatcacac 4740
gatgggggctaagcactggttcccgttacacctcctccgaaatagggtccgcctagtgtg
^ * ^ * ^ * ^ * ^ * ^ *
EciI BsaI
\ \
4741 tttcgagggcacctgtcgaatgggcggacaggagcacttctatctggagacccatgctgc 4800
aaagctcccgtggacagcttacccgcctgtcctcgtgaagatagacctctgggtacgacg
^ * ^ * ^ * ^ * ^ * ^ *
AceIII
BpmI BssSI
\ \ \
4801 attggccgtacctcgtgacagcgatgagctggaactcttttgctccacgcagcatccctc 4860
taaccggcatggagcactgtcgctactcgaccttgagaaaacgaggtgcgtcgtagggag
^ * ^ * ^ * ^ * ^ * ^ *
BsgI BstAPI
\ \
4861 ggaggtgcagaagctagtggcccatgtaaccgcacttcctgcccaccgtgtcgtctgtcg 4920
cctccacgtcttcgatcaccgggtacattggcgtgaaggacgggtggcacagcagacagc
^ * ^ * ^ * ^ * ^ * ^ *
BstXI BspGI
\ \
4921 tgccaagcgtttgggaggcggtttcggcggcaaggagtccagaggcatctccgtggccct 4980
acggttcgcaaaccctccgccaaagccgccgttcctcaggtctccgtagaggcaccggga
^ * ^ * ^ * ^ * ^ * ^ *
NruI
\
4981 acccgttgccctggccgcctatcgaatgggtcgtcctgtgcgctgtatgttggatcgcga 5040
tgggcaacgggaccggcggatagcttacccagcaggacacgcgacatacaacctagcgct
^ * ^ * ^ * ^ * ^ * ^ *
EarI Bsp24I
\ \
5041 tgaggacatgcttatcaccggcaccaggcatcccttcctcttcaaatacaaagtgggctt 5100
actcctgtacgaatagtggccgtggtccgtagggaaggagaagtttatgtttcacccgaa
^ * ^ * ^ * ^ * ^ * ^ *
HgiEII BclI Bsp24I NcoI
\ \ \ \
5101 caccaaggagggtctgatcactgcctgcgacattgagtgctacaacaatgccggttggtc 5160
gtggttcctcccagactagtgacggacgctgtaactcacgatgttgttacggccaaccag
^ * ^ * ^ * ^ * ^ * ^ *
NdeI
\
5161 catggatctgtcattttcggtaagagtggtgggtttatggaaaatccatatgtaggtttc 5220
gtacctagacagtaaaagccattctcaccacccaaataccttttaggtatacatccaaag
^ * ^ * ^ * ^ * ^ * ^ *
RleAI
NsiI BsbI XcmI
\ \ \\
5221 tttatgcataccatgtttgctcaaatatccatgtgttgtattttccattgaacatctggt 5280
aaatacgtatggtacaaacgagtttataggtacacaacataaaaggtaacttgtagacca
^ * ^ * ^ * ^ * ^ * ^ *
5281 tagtgtgggaatttctaggacccctatatgttatcatcctgagtgcaaatagtttgaaac 5340
atcacacccttaaagatcctggggatatacaatagtaggactcacgtttatcaaactttg
^ * ^ * ^ * ^ * ^ * ^ *
5341 ttttaagaatgtttaggtctaatcaggaaagcaagcaccaggattatattgacataaaca 5400
aaaattcttacaaatccagattagtcctttcgttcgtggtcctaatataactgtatttgt
^ * ^ * ^ * ^ * ^ * ^ *
5401 attataaataataatctaaaaattcttaaaaatgatctaatatataaatcctatgtttag 5460
taatatttattattagatttttaagaatttttactagattatatatttaggatacaaatc
^ * ^ * ^ * ^ * ^ * ^ *
AclI
BssHII Bce83I TaqII XcmI
\ \ \ \ \
5461 gttcttgagcgcgccatgttccactttgagaattgctacaggattcccaacgttcgcgtg 5520
caagaactcgcgcggtacaaggtgaaactcttaacgatgtcctaagggttgcaagcgcac
^ * ^ * ^ * ^ * ^ * ^ *
BspMI
\
5521 ggtggatgggtctgcaagacgaacctgccctcgaatacggccttccgtggatttggagga 5580
ccacctacccagacgttctgcttggacgggagcttatgccggaaggcacctaaacctcct
^ * ^ * ^ * ^ * ^ * ^ *
SgrAI NruI
\ \
5581 ccacaaggcatgtacgccggtgagcatatcatccgggatgtggcccggatagtgggtcgc 5640
ggtgttccgtacatgcggccactcgtatagtaggccctacaccgggcctatcacccagcg
^ * ^ * ^ * ^ * ^ * ^ *
BpmI
\
5641 gatgtggtggatgtgatgcggctgaacttctacaagactggagactacacacactaccac 5700
ctacaccacctacactacgccgacttgaagatgttctgacctctgatgtgtgtgatggtg
^ * ^ * ^ * ^ * ^ * ^ *
PvuII
AlwNI BsrBI
PflMI BpmI BpmI
\ \\ \
5701 cagcagctggagcacttccccatcgagcggtgtctggaggattgcttgaagcagtcgaga 5760
gtcgtcgacctcgtgaaggggtagctcgccacagacctcctaacgaacttcgtcagctct
^ * ^ * ^ * ^ * ^ * ^ *
EciI
\
5761 tacgacgagaagcggcaggatattgctcgattcaatcgggagaatcgctggcggaaacgc 5820
atgctgctcttcgccgtcctataacgagctaagttagccctcttagcgaccgcctttgcg
^ * ^ * ^ * ^ * ^ * ^ *
DrdII
\
5821 ggcatggcggtggtgcccaccaagtatggaatcgcatttggagtgatgcacttgaaccaa 5880
ccgtaccgccaccacgggtggttcataccttagcgtaaacctcactacgtgaacttggtt
^ * ^ * ^ * ^ * ^ * ^ *
BclI BsaBI BamHI BsbI
\ \ \ \
5881 gcgggatcgctgatcaacatctatggtgatggatccgtgttgctttcgcacggaggagtt 5940
cgccctagcgactagttgtagataccactacctaggcacaacgaaagcgtgcctcctcaa
^ * ^ * ^ * ^ * ^ * ^ *
Tth111I
BseRI BglI BstXI
\ \ \ \
5941 gagatcggacaaggtctgaataccaagatgattcagtgcgccgccagggctctggggatt 6000
ctctagcctgttccagacttatggttctactaagtcacgcggcggtcccgagacccctaa
^ * ^ * ^ * ^ * ^ * ^ *
XmnI BsbI RleAI
\ \ \
6001 ccttcggaactgattcacatttcggaaacggccacggataaagtacccaacacttcaccc 6060
ggaagccttgactaagtgtaaagcctttgccggtgcctatttcatgggttgtgaagtggg
^ * ^ * ^ * ^ * ^ * ^ *
BamHI
TaqII
\ \
6061 acggcggcgagtgtgggatccgatctgaacggaatggccgtactggatgcgtgtgaaaag 6120
tgccgccgctcacaccctaggctagacttgccttaccggcatgacctacgcacacttttc
^ * ^ * ^ * ^ * ^ * ^ *
NarI BsrDI BpmI
\ \ \
6121 ttgaacaaaagactggcgcccatcaaggaggcattgcctggaggcacctggaaggagtgg 6180
aacttgttttctgaccgcgggtagttcctccgtaacggacctccgtggaccttcctcacc
^ * ^ * ^ * ^ * ^ * ^ *
PvuI SmaI
\ \
6181 atcaacaaggcgtatttcgatcgggtcagcctctcggccacaggattctatgccatgccc 6240
tagttgttccgcataaagctagcccagtcggagagccggtgtcctaagatacggtacggg
^ * ^ * ^ * ^ * ^ * ^ *
EcoRV
\
6241 gggattggatatcacccggaaacgaatcccaatgctcgcacctatagctactacacgaat 6300
ccctaacctatagtgggcctttgcttagggttacgagcgtggatatcgatgatgtgctta
^ * ^ * ^ * ^ * ^ * ^ *
BsaXI ClaI BglI BcgI Bpu1102I
\ \ \ \ \
6301 ggcgtgggagtcactgtggtagagatcgattgcctgactggcgaccatcaggtgctcagc 6360
ccgcaccctcagtgacaccatctctagctaacggactgaccgctggtagtccacgagtcg
^ * ^ * ^ * ^ * ^ * ^ *
BsaBI BcgI PshAI
\ \ \
6361 acagacatcgtgatggacatcggctctagcctgaatccggctattgacattggtcagatc 6420
tgtctgtagcactacctgtagccgagatcggacttaggccgataactgtaaccagtctag
^ * ^ * ^ * ^ * ^ * ^ *
BsmI BpmI
\ \
6421 gagggagcattcatgcagggctatggactgttcactttggaggaactcatgtactcacca 6480
ctccctcgtaagtacgtcccgatacctgacaagtgaaacctccttgagtacatgagtggt
^ * ^ * ^ * ^ * ^ * ^ *
SphI BsrGI
\ \
6481 caaggcatgctttactccagaggtccgggcatgtacaagctgccaggatttgccgacatt 6540
gttccgtacgaaatgaggtctccaggcccgtacatgttcgacggtcctaaacggctgtaa
^ * ^ * ^ * ^ * ^ * ^ *
Bsp24I
SmaI PinAI TaqII
\ \ \ \
6541 cccggggagttcaatgtcagcctactgaccggtgcccccaatccacgggcagtctactct 6600
gggcccctcaagttacagtcggatgactggccacgggggttaggtgcccgtcagatgaga
^ * ^ * ^ * ^ * ^ * ^ *
EarI BstXI EarI
Bsp24I BsrBI SapI BsmI BstAPI
\ \ \ \ \ \ \
6601 tccaaggcagtgggtgaacctccgctcttcattggatcatctgcattctttgccattaag 6660
aggttccgtcacccacttggaggcgagaagtaacctagtagacgtaagaaacggtaattc
^ * ^ * ^ * ^ * ^ * ^ *
NarI
BsrDI PvuII NruI Bce83I
\ \ \ \ \
6661 gaggccattgcagctgctcgcgaggatcagggcttgagtggtgacttcccactggaggcg 6720
ctccggtaacgtcgacgagcgctcctagtcccgaactcaccactgaagggtgacctccgc
^ * ^ * ^ * ^ * ^ * ^ *
BsmI
BpmI NspV
\ \ \
6721 ccttccacatcggcacgcattcgaattgcttgtcaggataagttcacggaactggtaagt 6780
ggaaggtgtagccgtgcgtaagcttaacgaacagtcctattcaagtgccttgaccattca
^ * ^ * ^ * ^ * ^ * ^ *
VspI VspI
\ \
6781 tacccttggattagtttagaacattaaactaacttttattattaatttattaattatagc 6840
atgggaacctaatcaaatcttgtaatttgattgaaaataataattaaataattaatatcg
^ * ^ * ^ * ^ * ^ * ^ *
DrdII NcoI
\ \
6841 ttgaaatacccgaaccaggatcatttacgccatggaacattgtgccttaaaattgttttt 6900
aactttatgggcttggtcctagtaaatgcggtaccttgtaacacggaattttaacaaaaa
^ * ^ * ^ * ^ * ^ * ^ *
AflII DraI
\ \
6901 attgtttgtactgcttaagcatttaaaatgacgattttatttgttaatttgtttatatat 6960
taacaaacatgacgaattcgtaaattttactgctaaaataaacaattaaacaaatatata
^ * ^ * ^ * ^ * ^ * ^ *
DraI AceIII
\ \
6961 acatagtgaattaaatgttttaaaaataattgagtcgtttaatgtgtaaactaagctgga 7020
tgtatcacttaatttacaaaatttttattaactcagcaaattacacatttgattcgacct
^ * ^ * ^ * ^ * ^ * ^ *
SspI
BpmI VspI SspI
\ \ \ \
7021 gagctgtttgaacaaatttatataccactgattaatattaaatattccttttagattaag 7080
ctcgacaaacttgtttaaatatatggtgactaattataatttataaggaaaatctaattc
^ * ^ * ^ * ^ * ^ * ^ *
SphI
BssSI BstAPI
\ \ \
7081 aaaaattcaatggttacttttttgttctatctattattcaatagaatctcgtggcatgca 7140
tttttaagttaccaatgaaaaaacaagatagataataagttatcttagagcaccgtacgt
^ * ^ * ^ * ^ * ^ * ^ *
BsmI
NsiI
BsrDI MunI VspI
\ \ \ \
7141 atgcattctgattttcaattgaaaagtcttagtcactatttattttgagtatattaatga 7200
tacgtaagactaaaagttaacttttcagaatcagtgataaataaaactcatataattact
^ * ^ * ^ * ^ * ^ * ^ *
7201 aagttgagcaagttttccgatgaattgaaatatgagttccgatttcggcgcgactgctac 7260
ttcaactcgttcaaaaggctacttaactttatactcaaggctaaagccgcgctgacgatg
^ * ^ * ^ * ^ * ^ * ^ *
HindIII
\
7261 ccgaaaatataagctcaatcaaaagcaagctt 7320
ggcttttatattcgagttagttttcgttcgaa
^ * ^ * ^ * ^ * ^ * ^ *