LOCUS       PGSry       11012 bp    DNA   circular  INV       04-FEB-2001
DEFINITION  pPGS_ry.
ACCESSION   PGSry
VERSION
KEYWORDS    .
SOURCE      fruit fly.
  ORGANISM  Drosophila melanogaster
            Eukaryota; Metazoa; Arthropoda; Tracheata; Hexapoda; Insecta;
            Pterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
            Drosophilidae; Drosophila.
REFERENCE   1  (bases 1 to 11012)
  AUTHORS   Gunnar,S. and Gunter,R.
  TITLE     Controlled expression of tagged proteins in Drosophila using a new
            modular P-element vector system
  JOURNAL   Mol Gen Genet 262 (6), 916-920 (2000)
REFERENCE   2  (bases 1 to 11012)
  AUTHORS   Gunnar,S. and Gunter,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2001) Institute of Genetics,
            Martin-Luther-University Halle, Weinbergweg 10, Halle 06120,
            Germany
FEATURES             Location/Qualifiers
     source          1..11012
                     /organism="Drosophila melanogaster"
     misc_feature    1..541
                     /function="5' P repeat"
     misc_feature    542..652
                     /function="multi cloning site"
     misc_feature    653..7944
                     /function="rosy module"
     misc_feature    7945..8149
                     /function="3' P repeat"
     misc_feature    8150..11012
                     /function="pBluescript II"
BASE COUNT     2855 a   2511 c   2725 g   2921 t
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gaaagccgca gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 atctagaact agtccgcgga gcgctgggcc catagcggcc gcctcgaggc agaattccct
      601 gcaggatcga tggatccggc gcgccgtcga cgacgtcgct agcataccta ggaagcttta
      661 actatgttta atctcccata gagcttcgtt atcttcggac gcgatgtggc gcagatcccg
      721 tgctttcgta acagttttct ctacggaatc agcggaggga tcggcatcgg cttgctgact
      781 tttctgggca cctcgcgaac ccacctgtcc acccacgtgg gcttcggctc cttcttctgc
      841 ggcaccatcg cctactggat gacctgcagg taagtgactg tgattgaaac cgtgcgtcgc
      901 ccttatcact taattatcaa tgtggcatcc gcaggtatca atggtccgtc aggagattcg
      961 agcagcagca attgcgtgag gcgatgagac gacaagccct ttatgagggc acccaaaggg
     1021 agagagactt agatctgaag tcggcgtagc ataagtcaga tccttcatta ctacttcgat
     1081 gtttgttttc actctaggat accaagtaat cctggcattg tcaaatccct tgtgttgtaa
     1141 actacctttt gttaccaaac tttatagtag ccaatatatt atatatatgt tgtaagtgaa
     1201 atcatagctt ggagtttgct tgccactcgt agggaattag ttatgccacc atctttgatt
     1261 tgggattgac agaaatcaat ccatcatttt aatgattgtg gttcctaaaa cctgttgggc
     1321 tttattgtgc tttcgatttg atttaagctg atagagctag ttgtttactt ttgaagaaca
     1381 ttgaacccat tttgacgacc ttttaacttt ttaatagttt ttttttacca tttacataaa
     1441 agcaaaattt acagtctaaa agttgtatat tcaatacgtt ctggtacgta aaagggttat
     1501 ttaatgcacc atttttacaa ctttagatag tatacttttt acttgagtgg gagccatatc
     1561 acaaaaatga aatgaaaggt taaaaaagta tctaaaagta agtttcaatt aatattctga
     1621 aaaataattg catttcatgt acgtttctct gaaatacccc taattcgata ctatttatag
     1681 atctggaaga tatatagcta ggaaatagaa ttatatattt gcctagtttg aacaccttgt
     1741 gaacattttg tttaaaaaaa aagcgtatgg tggtactaga aaactttaag atacagataa
     1801 ataacttcac tataccgagg ttttggtgtt tcttgagact gaaggacaat cttcaaaatg
     1861 gtcttaattc ttgagataga aaagctgaag ttctcatgct gaatagtgat cagtacgatt
     1921 ttatctgaca gattgctttc ctagctgatc ataccgatct cggatgggta tctgctctaa
     1981 tccgagatag caggtagtag aactgctgcc tcgtaacccc tagacatggc catttccacg
     2041 cacatgtagg cgatcctgca ctcgtcggtc tctcgtctta tctcacaaaa ataaaagtcg
     2101 gcgtttggat ggattgctcg gctggctgtt ggcacattca ttacgttttc gttgacggcg
     2161 cgagacggtt gtgttgtttt ttaatcggcg ggcacatgag gttgtcggtt ctctaatttt
     2221 gaaataaaaa acttgttttc gctttacatt caaggaacgc acctcctccc ctccatatat
     2281 gtaaacgagc ttcgtaattg caggctaaca cttatatagg cattggtact gagatacagc
     2341 gagataccgt gagctacaga tacatagcac ttcacgatgt ctaactcggt tttggtgttt
     2401 ttcgtgaatg gaaagaaggt acttaaagat actcggatgc cttgcgtgtc tttccttaaa
     2461 gtcggcccac tcttactata tcgattccct ttattgtttt attgccctaa ttgcgtttaa
     2521 ctggcattcg atggaatttg ccttcttgct caattgaatg tgtcgaatgt atatagtatt
     2581 tgctgtgcat atagtgataa caattgaccc ccatgtgaag cctcgttgtt tgctggtggc
     2641 cccttatcgc ccgatcaaga tggtgcactt gccaatcgcg catgggagca gtgctactcg
     2701 aatgtgggtg agctgggatc gtcatgggca tttgtgtctg ccagatagct caggccctct
     2761 aatccgcaca ttcccaccgg ctgggcggtg tttacaatcc agtgagctcg gctggattat
     2821 tcagataaag ctggccatat ttagcatctg gtgttggtgg agtgcaattg gagttcttta
     2881 ctttgtgctc acagcttgcg cacaagtgaa gtaaataata ataaataacc agttgctcgt
     2941 gcgcccggca tttgatagtg atagatagca atctctgttt gctcagggct ggcagagcag
     3001 ataacacttt cgctgtttta agtttaaaca aagtcacagt tttgacaaac aattgcttta
     3061 aaccatataa acttataatt aaagcaatta taatgggtta agtaagcaca gatctagcta
     3121 caagatacaa gatacaaatt ttccaattaa tggataaatg gcattagata cttttgggaa
     3181 attgatttcg tttttttcat tttattatat ttatacattt cttattccag gtgaccgaag
     3241 tgtcgcctga tccggagtgc acgctcctca cattcctgcg cgaaaagctg cggctgtgcg
     3301 gaacgaagtt gggatgtgcg gaaggcggat gcggcgcctg caccgtgatg gtgtcccgcc
     3361 tagaccgtcg ggccaacaag atacgtcacc tggcggtcaa cgcctgcctg acgccagtgt
     3421 gctcgatgca cggatgtgcg gtgaccactg tggagggtat agggagcacc aaaacgcgtc
     3481 tgcatccggt gcaggagcga ctgcccaagg cgcacggatc ccagtgcggc ttctgcacgc
     3541 cgggcattgt gatgtccatg tacgcacttc tgcggaacgc ggagcaaccc tctatgcgag
     3601 acttggaggt ggcattccaa ggtaacctgt gccgctgcac cggctatcga cccattctcg
     3661 agggctacaa gacgttcacc aaggagtttg cctgcggaat gggcgagaag tgctgcaaag
     3721 ttagtgggaa aggatgtgga accgatgcgg agaccgatga caagctcttc gagcgcagcg
     3781 aattccagcc cttggatccc agccaggaac ccatcttccc accggaactt cagctgagtg
     3841 acgccttcga ttcgcagagt ttgatcttta gttcggatag ggtgacctgg tatcgtccca
     3901 ccaatctgga ggagctgctt cagctgaagg ccaaacatcc ggctgccaag ctggtcgtgg
     3961 gcaatacgga agtgggcgtt gaggttaagt tcaagcactt cctctacccg cacctcatca
     4021 atcccaccca ggtgaaggag ctgctggaga tcaaagagaa ccaggatggc atttacttcg
     4081 gtgcggctgt cagtttgatg gagatcgatg cgcttctgcg gcagagaatc gagctgctgc
     4141 cggaatcgga gaccagattg ttccagtgca ccgtggatat gcttcactac tttgccggca
     4201 agcagatccg caacgtcgcc tgtttgggtg gaaacatcat gaccggcagt cccatttccg
     4261 atatgaatcc tgtgctctcg gcagcaggag ctcaactgga ggtggccagt tttgtggatg
     4321 gaaagctcca aaagagatca gttcacatgg gaactgggtt cttcactggc tatcgcagga
     4381 atgttatcga agcccacgag gtgctgctgg gcatccactt tcggaagacc actccggacc
     4441 agtatatcgt tgcttttaag caggccagaa gaagggatga tgacatagcc atcgtaaatg
     4501 ccgcaataaa cgttcgcttt gaggaaaaat ccaacattgt ggcggagatc tcgatggctt
     4561 ttggtggaat ggcaccaacc acagtgctcg ctcctcgaac ttcccaactg atggttgggc
     4621 aggagtggag ccaccagctc gtggagcgcg tggcggagag cttgtgcacg gagctgcctt
     4681 tggctgcctc cgctccgggt ggcatgatcg cctatcgtcg agctctggtg gtgagcctgt
     4741 tcttcaaggc ctatctggcc atttccctga agctgagcaa gtcagggatc acatcttccg
     4801 atgctctgcc accggaggag cgaagtggtg ccgagacatt ccacacacca gtactcaaaa
     4861 gtgcccagct cttcgagcgc gtctgcagcg atcaacccat ctgtgatccc attgggagac
     4921 caaaagttca tgcggctgct ttgaaacagg ccactggtga agctatctac acagatgaca
     4981 ttccccgcat ggatggtgaa gtttatctgg cctttgtcct tagtaccaag ccacgtgcca
     5041 agatcaccaa gctggatgcc agtgaagctc tggccctgga cggagtgcat cagttctttt
     5101 gctacaagga cttaacggag cacgagaacg aagtgggacc cgtctttcat gatgagcacg
     5161 tctttgccgc tggagaagtg cattgctatg gtcagatagt gggcgccata gctgccgata
     5221 ataaggcgtt ggcccaaaga gccgctcggc tagtgaaagt ggagtacgag gagctgagcc
     5281 cggttatcgt gaccatagag caggccatcg agctcaagtc ctatttcccg gactaccccc
     5341 gattcgtgac caagggcaat gtggaggagg ctttatccca ggcggatcac actttcgagg
     5401 gcacctgtcg aatgggcgga caggagcact tctatctgga gacccatgct gcattggccg
     5461 tacctcgtga cagcgatgag ctggaactct tttgctccac gcagcatccc tcggaggtgc
     5521 agaagctagt ggcccatgta accgcacttc ctgcccaccg tgtcgtctgt cgtgccaagc
     5581 gtttgggagg cggtttcggc ggcaaggagt ccagaggcat ctccgtggcc ctacccgttg
     5641 ccctggccgc ctatcgaatg ggtcgtcctg tgcgctgtat gttggatcgc gatgaggaca
     5701 tgcttatcac cggcaccagg catcccttcc tcttcaaata caaagtgggc ttcaccaagg
     5761 agggtctgat cactgcctgc gacattgagt gctacaacaa tgccggttgg tccatggatc
     5821 tgtcattttc ggtaagagtg gtgggtttat ggaaaatcca tatgtaggtt tctttatgca
     5881 taccatgttt gctcaaatat ccatgtgttg tattttccat tgaacatctg gttagtgtgg
     5941 gaatttctag gacccctata tgttatcatc ctgagtgcaa atagtttgaa acttttaaga
     6001 atgtttaggt ctaatcagga aagcaagcac caggattata ttgacataaa caattataaa
     6061 taataatcta aaaattctta aaaatgatct aatatataaa tcctatgttt aggttcttga
     6121 gcgcgccatg ttccactttg agaattgcta caggattccc aacgttcgcg tgggtggatg
     6181 ggtctgcaag acgaacctgc cctcgaatac ggccttccgt ggatttggag gaccacaagg
     6241 catgtacgcc ggtgagcata tcatccggga tgtggcccgg atagtgggtc gcgatgtggt
     6301 ggatgtgatg cggctgaact tctacaagac tggagactac acacactacc accagcagct
     6361 ggagcacttc cccatcgagc ggtgtctgga ggattgcttg aagcagtcga gatacgacga
     6421 gaagcggcag gatattgctc gattcaatcg ggagaatcgc tggcggaaac gcggcatggc
     6481 ggtggtgccc accaagtatg gaatcgcatt tggagtgatg cacttgaacc aagcgggatc
     6541 gctgatcaac atctatggtg atggatccgt gttgctttcg cacggaggag ttgagatcgg
     6601 acaaggtctg aataccaaga tgattcagtg cgccgccagg gctctgggga ttccttcgga
     6661 actgattcac atttcggaaa cggccacgga taaagtaccc aacacttcac ccacggcggc
     6721 gagtgtggga tccgatctga acggaatggc cgtactggat gcgtgtgaaa agttgaacaa
     6781 aagactggcg cccatcaagg aggcattgcc tggaggcacc tggaaggagt ggatcaacaa
     6841 ggcgtatttc gatcgggtca gcctctcggc cacaggattc tatgccatgc ccgggattgg
     6901 atatcacccg gaaacgaatc ccaatgctcg cacctatagc tactacacga atggcgtggg
     6961 agtcactgtg gtagagatcg attgcctgac tggcgaccat caggtgctca gcacagacat
     7021 cgtgatggac atcggctcta gcctgaatcc ggctattgac attggtcaga tcgagggagc
     7081 attcatgcag ggctatggac tgttcacttt ggaggaactc atgtactcac cacaaggcat
     7141 gctttactcc agaggtccgg gcatgtacaa gctgccagga tttgccgaca ttcccgggga
     7201 gttcaatgtc agcctactga ccggtgcccc caatccacgg gcagtctact cttccaaggc
     7261 agtgggtgaa cctccgctct tcattggatc atctgcattc tttgccatta aggaggccat
     7321 tgcagctgct cgcgaggatc agggcttgag tggtgacttc ccactggagg cgccttccac
     7381 atcggcacgc attcgaattg cttgtcagga taagttcacg gaactggtaa gttacccttg
     7441 gattagttta gaacattaaa ctaactttta ttattaattt attaattata gcttgaaata
     7501 cccgaaccag gatcatttac gccatggaac attgtgcctt aaaattgttt ttattgtttg
     7561 tactgcttaa gcatttaaaa tgacgatttt atttgttaat ttgtttatat atacatagtg
     7621 aattaaatgt tttaaaaata attgagtcgt ttaatgtgta aactaagctg gagagctgtt
     7681 tgaacaaatt tatataccac tgattaatat taaatattcc ttttagatta agaaaaattc
     7741 aatggttact tttttgttct atctattatt caatagaatc tcgtggcatg caatgcattc
     7801 tgattttcaa ttgaaaagtc ttagtcacta tttattttga gtatattaat gaaagttgag
     7861 caagttttcc gatgaattga aatatgagtt ccgatttcgg cgcgactgct acccgaaaat
     7921 ataagctcaa tcaaaagcaa gcttcaaatt attaaaaata aaactttaaa aataatttcg
     7981 tctaattaat attatgagtt aattcaaacc ccacggacat gctaagggtt aatcaacaat
     8041 catatcgctg tctcactcag actcaatacg acactcagaa tactattcct ttcactcgca
     8101 cttattgcaa gcatacgccg acgggaccac cttatgttat ttcatcatgg gtacccagct
     8161 tttgttccct ttagtgaggg ttaattccga gcttggcgta atcatggtca tagctgtttc
     8221 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat aggagccgga agcataaagt
     8281 gtaaagcctg gggtgcctaa tgagtgaggt aactcacatt aattgcgttg cgctcactgc
     8341 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8401 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8461 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8521 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8581 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct cggcccccct gacgagcatc
     8641 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8701 cgttcccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8761 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
     8821 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8881 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8941 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9001 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     9061 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9121 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9181 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9241 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9301 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9361 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9421 catccatagt tgcctgactg cccgtcgtgt agataactac gatacgggag ggcttaccat
     9481 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9541 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9601 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9661 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9721 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgaa
     9781 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9841 tatcactcat gcttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9901 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9961 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10021 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10081 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10141 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10201 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10261 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10321 taggggttcc gcgcacattt ccccgaaaag tgccacctga aattgtaaac gttaatattt
    10381 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
    10441 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
    10501 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
    10561 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
    10621 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
    10681 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
    10741 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
    10801 cgctacaggg cgcgtcccat tcgccattca ggctacgcaa ctgttgggaa gggcgatcgg
    10861 tgcgggcctc ttcgctatta cgccagctgg cgaagggggg atgtgctgca aggcgattaa
    10921 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt
    10981 aatacgactc actatagggc gaattggagc tc
//
