LOCUS       PGSryEGFP   11696 bp    DNA   circular  INV       04-FEB-2001
DEFINITION  pPGS_ry_EGFP.
ACCESSION   PGSryEGFP
VERSION
KEYWORDS    .
SOURCE      fruit fly.
  ORGANISM  Drosophila melanogaster
            Eukaryota; Metazoa; Arthropoda; Tracheata; Hexapoda; Insecta;
            Pterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
            Drosophilidae; Drosophila.
REFERENCE   1  (bases 1 to 11696)
  AUTHORS   Gunnar,S. and Gunter,R.
  TITLE     Controlled expression of tagged proteins in Drosophila using a new
            modular P-element vector system
  JOURNAL   Mol Gen Genet 262 (6), 916-920 (2000)
REFERENCE   2  (bases 1 to 11696)
  AUTHORS   Gunnar,S. and Gunter,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2001) Institute of Genetics,
            Martin-Luther-University Halle, Weinbergweg 10, Halle 06120,
            Germany
FEATURES             Location/Qualifiers
     source          1..11696
                     /organism="Drosophila melanogaster"
     misc_feature    1..541
                     /function="5' P repeat"
     misc_feature    join(542..574,1316..1336)
                     /function="multi cloning site"
     misc_feature    575..1315
                     /function="EGFP module"
     misc_feature    1337..8628
                     /function="rosy module"
     misc_feature    8629..8833
                     /function="3' P repeat"
     misc_feature    8834..11696
                     /function="pBluescript II"
BASE COUNT     3021 a   2741 c   2918 g   3016 t
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gaaagccgca gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 atctagaact agtccgcgga gcgctgggcc catagcggcc gcatggtgag caagggcgag
      601 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      661 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      721 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
      781 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      841 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      901 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      961 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1021 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     1081 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1141 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1201 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1261 gccgccggga tcactctcgg catggacgag ctgtacaagg cccgggcaag tcgacgacgt
     1321 cgctagcata cctaggaagc tttaactatg tttaatctcc catagagctt cgttatcttc
     1381 ggacgcgatg tggcgcagat cccgtgcttt cgtaacagtt ttctctacgg aatcagcgga
     1441 gggatcggca tcggcttgct gacttttctg ggcacctcgc gaacccacct gtccacccac
     1501 gtgggcttcg gctccttctt ctgcggcacc atcgcctact ggatgacctg caggtaagtg
     1561 actgtgattg aaaccgtgcg tcgcccttat cacttaatta tcaatgtggc atccgcaggt
     1621 atcaatggtc cgtcaggaga ttcgagcagc agcaattgcg tgaggcgatg agacgacaag
     1681 ccctttatga gggcacccaa agggagagag acttagatct gaagtcggcg tagcataagt
     1741 cagatccttc attactactt cgatgtttgt tttcactcta ggataccaag taatcctggc
     1801 attgtcaaat cccttgtgtt gtaaactacc ttttgttacc aaactttata gtagccaata
     1861 tattatatat atgttgtaag tgaaatcata gcttggagtt tgcttgccac tcgtagggaa
     1921 ttagttatgc caccatcttt gatttgggat tgacagaaat caatccatca ttttaatgat
     1981 tgtggttcct aaaacctgtt gggctttatt gtgctttcga tttgatttaa gctgatagag
     2041 ctagttgttt acttttgaag aacattgaac ccattttgac gaccttttaa ctttttaata
     2101 gttttttttt accatttaca taaaagcaaa atttacagtc taaaagttgt atattcaata
     2161 cgttctggta cgtaaaaggg ttatttaatg caccattttt acaactttag atagtatact
     2221 ttttacttga gtgggagcca tatcacaaaa atgaaatgaa aggttaaaaa agtatctaaa
     2281 agtaagtttc aattaatatt ctgaaaaata attgcatttc atgtacgttt ctctgaaata
     2341 cccctaattc gatactattt atagatctgg aagatatata gctaggaaat agaattatat
     2401 atttgcctag tttgaacacc ttgtgaacat tttgtttaaa aaaaaagcgt atggtggtac
     2461 tagaaaactt taagatacag ataaataact tcactatacc gaggttttgg tgtttcttga
     2521 gactgaagga caatcttcaa aatggtctta attcttgaga tagaaaagct gaagttctca
     2581 tgctgaatag tgatcagtac gattttatct gacagattgc tttcctagct gatcataccg
     2641 atctcggatg ggtatctgct ctaatccgag atagcaggta gtagaactgc tgcctcgtaa
     2701 cccctagaca tggccatttc cacgcacatg taggcgatcc tgcactcgtc ggtctctcgt
     2761 cttatctcac aaaaataaaa gtcggcgttt ggatggattg ctcggctggc tgttggcaca
     2821 ttcattacgt tttcgttgac ggcgcgagac ggttgtgttg ttttttaatc ggcgggcaca
     2881 tgaggttgtc ggttctctaa ttttgaaata aaaaacttgt tttcgcttta cattcaagga
     2941 acgcacctcc tcccctccat atatgtaaac gagcttcgta attgcaggct aacacttata
     3001 taggcattgg tactgagata cagcgagata ccgtgagcta cagatacata gcacttcacg
     3061 atgtctaact cggttttggt gtttttcgtg aatggaaaga aggtacttaa agatactcgg
     3121 atgccttgcg tgtctttcct taaagtcggc ccactcttac tatatcgatt ccctttattg
     3181 ttttattgcc ctaattgcgt ttaactggca ttcgatggaa tttgccttct tgctcaattg
     3241 aatgtgtcga atgtatatag tatttgctgt gcatatagtg ataacaattg acccccatgt
     3301 gaagcctcgt tgtttgctgg tggcccctta tcgcccgatc aagatggtgc acttgccaat
     3361 cgcgcatggg agcagtgcta ctcgaatgtg ggtgagctgg gatcgtcatg ggcatttgtg
     3421 tctgccagat agctcaggcc ctctaatccg cacattccca ccggctgggc ggtgtttaca
     3481 atccagtgag ctcggctgga ttattcagat aaagctggcc atatttagca tctggtgttg
     3541 gtggagtgca attggagttc tttactttgt gctcacagct tgcgcacaag tgaagtaaat
     3601 aataataaat aaccagttgc tcgtgcgccc ggcatttgat agtgatagat agcaatctct
     3661 gtttgctcag ggctggcaga gcagataaca ctttcgctgt tttaagttta aacaaagtca
     3721 cagttttgac aaacaattgc tttaaaccat ataaacttat aattaaagca attataatgg
     3781 gttaagtaag cacagatcta gctacaagat acaagataca aattttccaa ttaatggata
     3841 aatggcatta gatacttttg ggaaattgat ttcgtttttt tcattttatt atatttatac
     3901 atttcttatt ccaggtgacc gaagtgtcgc ctgatccgga gtgcacgctc ctcacattcc
     3961 tgcgcgaaaa gctgcggctg tgcggaacga agttgggatg tgcggaaggc ggatgcggcg
     4021 cctgcaccgt gatggtgtcc cgcctagacc gtcgggccaa caagatacgt cacctggcgg
     4081 tcaacgcctg cctgacgcca gtgtgctcga tgcacggatg tgcggtgacc actgtggagg
     4141 gtatagggag caccaaaacg cgtctgcatc cggtgcagga gcgactgccc aaggcgcacg
     4201 gatcccagtg cggcttctgc acgccgggca ttgtgatgtc catgtacgca cttctgcgga
     4261 acgcggagca accctctatg cgagacttgg aggtggcatt ccaaggtaac ctgtgccgct
     4321 gcaccggcta tcgacccatt ctcgagggct acaagacgtt caccaaggag tttgcctgcg
     4381 gaatgggcga gaagtgctgc aaagttagtg ggaaaggatg tggaaccgat gcggagaccg
     4441 atgacaagct cttcgagcgc agcgaattcc agcccttgga tcccagccag gaacccatct
     4501 tcccaccgga acttcagctg agtgacgcct tcgattcgca gagtttgatc tttagttcgg
     4561 atagggtgac ctggtatcgt cccaccaatc tggaggagct gcttcagctg aaggccaaac
     4621 atccggctgc caagctggtc gtgggcaata cggaagtggg cgttgaggtt aagttcaagc
     4681 acttcctcta cccgcacctc atcaatccca cccaggtgaa ggagctgctg gagatcaaag
     4741 agaaccagga tggcatttac ttcggtgcgg ctgtcagttt gatggagatc gatgcgcttc
     4801 tgcggcagag aatcgagctg ctgccggaat cggagaccag attgttccag tgcaccgtgg
     4861 atatgcttca ctactttgcc ggcaagcaga tccgcaacgt cgcctgtttg ggtggaaaca
     4921 tcatgaccgg cagtcccatt tccgatatga atcctgtgct ctcggcagca ggagctcaac
     4981 tggaggtggc cagttttgtg gatggaaagc tccaaaagag atcagttcac atgggaactg
     5041 ggttcttcac tggctatcgc aggaatgtta tcgaagccca cgaggtgctg ctgggcatcc
     5101 actttcggaa gaccactccg gaccagtata tcgttgcttt taagcaggcc agaagaaggg
     5161 atgatgacat agccatcgta aatgccgcaa taaacgttcg ctttgaggaa aaatccaaca
     5221 ttgtggcgga gatctcgatg gcttttggtg gaatggcacc aaccacagtg ctcgctcctc
     5281 gaacttccca actgatggtt gggcaggagt ggagccacca gctcgtggag cgcgtggcgg
     5341 agagcttgtg cacggagctg cctttggctg cctccgctcc gggtggcatg atcgcctatc
     5401 gtcgagctct ggtggtgagc ctgttcttca aggcctatct ggccatttcc ctgaagctga
     5461 gcaagtcagg gatcacatct tccgatgctc tgccaccgga ggagcgaagt ggtgccgaga
     5521 cattccacac accagtactc aaaagtgccc agctcttcga gcgcgtctgc agcgatcaac
     5581 ccatctgtga tcccattggg agaccaaaag ttcatgcggc tgctttgaaa caggccactg
     5641 gtgaagctat ctacacagat gacattcccc gcatggatgg tgaagtttat ctggcctttg
     5701 tccttagtac caagccacgt gccaagatca ccaagctgga tgccagtgaa gctctggccc
     5761 tggacggagt gcatcagttc ttttgctaca aggacttaac ggagcacgag aacgaagtgg
     5821 gacccgtctt tcatgatgag cacgtctttg ccgctggaga agtgcattgc tatggtcaga
     5881 tagtgggcgc catagctgcc gataataagg cgttggccca aagagccgct cggctagtga
     5941 aagtggagta cgaggagctg agcccggtta tcgtgaccat agagcaggcc atcgagctca
     6001 agtcctattt cccggactac ccccgattcg tgaccaaggg caatgtggag gaggctttat
     6061 cccaggcgga tcacactttc gagggcacct gtcgaatggg cggacaggag cacttctatc
     6121 tggagaccca tgctgcattg gccgtacctc gtgacagcga tgagctggaa ctcttttgct
     6181 ccacgcagca tccctcggag gtgcagaagc tagtggccca tgtaaccgca cttcctgccc
     6241 accgtgtcgt ctgtcgtgcc aagcgtttgg gaggcggttt cggcggcaag gagtccagag
     6301 gcatctccgt ggccctaccc gttgccctgg ccgcctatcg aatgggtcgt cctgtgcgct
     6361 gtatgttgga tcgcgatgag gacatgctta tcaccggcac caggcatccc ttcctcttca
     6421 aatacaaagt gggcttcacc aaggagggtc tgatcactgc ctgcgacatt gagtgctaca
     6481 acaatgccgg ttggtccatg gatctgtcat tttcggtaag agtggtgggt ttatggaaaa
     6541 tccatatgta ggtttcttta tgcataccat gtttgctcaa atatccatgt gttgtatttt
     6601 ccattgaaca tctggttagt gtgggaattt ctaggacccc tatatgttat catcctgagt
     6661 gcaaatagtt tgaaactttt aagaatgttt aggtctaatc aggaaagcaa gcaccaggat
     6721 tatattgaca taaacaatta taaataataa tctaaaaatt cttaaaaatg atctaatata
     6781 taaatcctat gtttaggttc ttgagcgcgc catgttccac tttgagaatt gctacaggat
     6841 tcccaacgtt cgcgtgggtg gatgggtctg caagacgaac ctgccctcga atacggcctt
     6901 ccgtggattt ggaggaccac aaggcatgta cgccggtgag catatcatcc gggatgtggc
     6961 ccggatagtg ggtcgcgatg tggtggatgt gatgcggctg aacttctaca agactggaga
     7021 ctacacacac taccaccagc agctggagca cttccccatc gagcggtgtc tggaggattg
     7081 cttgaagcag tcgagatacg acgagaagcg gcaggatatt gctcgattca atcgggagaa
     7141 tcgctggcgg aaacgcggca tggcggtggt gcccaccaag tatggaatcg catttggagt
     7201 gatgcacttg aaccaagcgg gatcgctgat caacatctat ggtgatggat ccgtgttgct
     7261 ttcgcacgga ggagttgaga tcggacaagg tctgaatacc aagatgattc agtgcgccgc
     7321 cagggctctg gggattcctt cggaactgat tcacatttcg gaaacggcca cggataaagt
     7381 acccaacact tcacccacgg cggcgagtgt gggatccgat ctgaacggaa tggccgtact
     7441 ggatgcgtgt gaaaagttga acaaaagact ggcgcccatc aaggaggcat tgcctggagg
     7501 cacctggaag gagtggatca acaaggcgta tttcgatcgg gtcagcctct cggccacagg
     7561 attctatgcc atgcccggga ttggatatca cccggaaacg aatcccaatg ctcgcaccta
     7621 tagctactac acgaatggcg tgggagtcac tgtggtagag atcgattgcc tgactggcga
     7681 ccatcaggtg ctcagcacag acatcgtgat ggacatcggc tctagcctga atccggctat
     7741 tgacattggt cagatcgagg gagcattcat gcagggctat ggactgttca ctttggagga
     7801 actcatgtac tcaccacaag gcatgcttta ctccagaggt ccgggcatgt acaagctgcc
     7861 aggatttgcc gacattcccg gggagttcaa tgtcagccta ctgaccggtg cccccaatcc
     7921 acgggcagtc tactcttcca aggcagtggg tgaacctccg ctcttcattg gatcatctgc
     7981 attctttgcc attaaggagg ccattgcagc tgctcgcgag gatcagggct tgagtggtga
     8041 cttcccactg gaggcgcctt ccacatcggc acgcattcga attgcttgtc aggataagtt
     8101 cacggaactg gtaagttacc cttggattag tttagaacat taaactaact tttattatta
     8161 atttattaat tatagcttga aatacccgaa ccaggatcat ttacgccatg gaacattgtg
     8221 ccttaaaatt gtttttattg tttgtactgc ttaagcattt aaaatgacga ttttatttgt
     8281 taatttgttt atatatacat agtgaattaa atgttttaaa aataattgag tcgtttaatg
     8341 tgtaaactaa gctggagagc tgtttgaaca aatttatata ccactgatta atattaaata
     8401 ttccttttag attaagaaaa attcaatggt tacttttttg ttctatctat tattcaatag
     8461 aatctcgtgg catgcaatgc attctgattt tcaattgaaa agtcttagtc actatttatt
     8521 ttgagtatat taatgaaagt tgagcaagtt ttccgatgaa ttgaaatatg agttccgatt
     8581 tcggcgcgac tgctacccga aaatataagc tcaatcaaaa gcaagcttca aattattaaa
     8641 aataaaactt taaaaataat ttcgtctaat taatattatg agttaattca aaccccacgg
     8701 acatgctaag ggttaatcaa caatcatatc gctgtctcac tcagactcaa tacgacactc
     8761 agaatactat tcctttcact cgcacttatt gcaagcatac gccgacggga ccaccttatg
     8821 ttatttcatc atgggtaccc agcttttgtt ccctttagtg agggttaatt ccgagcttgg
     8881 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     8941 acataggagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg aggtaactca
     9001 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     9061 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     9121 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     9181 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     9241 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     9301 ggctcggccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     9361 cgacaggact ataaagatac caggcgttcc cccctggaag ctccctcgtg cgctctcctg
     9421 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     9481 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     9541 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     9601 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     9661 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     9721 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     9781 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     9841 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9901 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9961 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    10021 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    10081 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actgcccgtc gtgtagataa
    10141 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    10201 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    10261 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    10321 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    10381 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    10441 ttacatgatc ccccatgttg tgaaaaaaag cggttagctc cttcggtcct ccgatcgttg
    10501 tcagaagtaa gttggccgca gtgttatcac tcatgcttat ggcagcactg cataattctc
    10561 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    10621 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    10681 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    10741 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    10801 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    10861 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    10921 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    10981 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    11041 ctgaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
    11101 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
    11161 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
    11221 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
    11281 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
    11341 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
    11401 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
    11461 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctac
    11521 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaagg
    11581 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
    11641 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg gagctc
//
