LOCUS       PGSrUEGFP3  12354 bp    DNA   circular  INV       04-FEB-2001
DEFINITION  pPGS_ry_UAS_EGFP_3.
ACCESSION   PGSrUEGFP3
VERSION
KEYWORDS    .
SOURCE      fruit fly.
  ORGANISM  Drosophila melanogaster
            Eukaryota; Metazoa; Arthropoda; Tracheata; Hexapoda; Insecta;
            Pterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
            Drosophilidae; Drosophila.
REFERENCE   1  (bases 1 to 12354)
  AUTHORS   Gunnar,S. and Gunter,R.
  TITLE     Controlled expression of tagged proteins in Drosophila using a new
            modular P-element vector system
  JOURNAL   Mol Gen Genet 262 (6), 916-920 (2000)
REFERENCE   2  (bases 1 to 12354)
  AUTHORS   Gunnar,S. and Gunter,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2001) Institute of Genetics,
            Martin-Luther-University Halle, Weinbergweg 10, Halle 06120,
            Germany
FEATURES             Location/Qualifiers
     source          1..12354
                     /organism="Drosophila melanogaster"
     misc_feature    1..541
                     /function="5' P repeat"
     misc_feature    542..924
                     /function="UAS module"
     misc_feature    join(925..927,1669..1683)
                     /function="multi cloning site"
     misc_feature    928..1668
                     /function="EGFP module"
     misc_feature    1684..1994
                     /function="3' polyA module"
     misc_feature    1995..9286
                     /function="rosy module"
     misc_feature    9287..9491
                     /function="3' P repeat"
     misc_feature    9492..12354
                     /function="pBluescript II"
BASE COUNT     3225 a   2899 c   3067 g   3163 t
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gaaagccgca gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 atctagatgc atgcctgcag gtcggagtac tgtcctccga gcggagtact gtcctccgag
      601 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
      661 agactctagc gagcgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa
      721 ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc
      781 tgaacaagct aaacaatctg cagtaaagtg caagttaaag tgaatcaatt aaaagtaacc
      841 agcaaccaag taaatcaact gcaactactg aaatctgcca agaagtaatt attgaataca
      901 agaagagaac tctgaatagg gcccatagcg gccgcatggt gagcaagggc gaggagctgt
      961 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     1021 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     1081 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     1141 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     1201 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     1261 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     1321 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     1381 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     1441 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     1501 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     1561 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     1621 ggatcactct cggcatggac gagctgtaca aggcccgggc aagtcgacga cgtcgctagc
     1681 atacctaggt agtttaaatt gattgttgca ttcgtaaacc gaaataaatg cctgtactag
     1741 aacttccctt taaacctatt gtgcactagt tagtggtctc acctggatct cccaaaataa
     1801 cctcaactaa tgtctgtcaa aacacatcta cctccctttc caggtgtcat ccagctgaag
     1861 gcgctcttcg agggcaaaac cacgggtgcc agtatccagt acaccacgac gcaacgaggc
     1921 agggtgatgc tggtctacga gggttatcgg tatgtcgtga atcgccagag cctgaagaac
     1981 gtcttctgcc taggaagctt taactatgtt taatctccca tagagcttcg ttatcttcgg
     2041 acgcgatgtg gcgcagatcc cgtgctttcg taacagtttt ctctacggaa tcagcggagg
     2101 gatcggcatc ggcttgctga cttttctggg cacctcgcga acccacctgt ccacccacgt
     2161 gggcttcggc tccttcttct gcggcaccat cgcctactgg atgacctgca ggtaagtgac
     2221 tgtgattgaa accgtgcgtc gcccttatca cttaattatc aatgtggcat ccgcaggtat
     2281 caatggtccg tcaggagatt cgagcagcag caattgcgtg aggcgatgag acgacaagcc
     2341 ctttatgagg gcacccaaag ggagagagac ttagatctga agtcggcgta gcataagtca
     2401 gatccttcat tactacttcg atgtttgttt tcactctagg ataccaagta atcctggcat
     2461 tgtcaaatcc cttgtgttgt aaactacctt ttgttaccaa actttatagt agccaatata
     2521 ttatatatat gttgtaagtg aaatcatagc ttggagtttg cttgccactc gtagggaatt
     2581 agttatgcca ccatctttga tttgggattg acagaaatca atccatcatt ttaatgattg
     2641 tggttcctaa aacctgttgg gctttattgt gctttcgatt tgatttaagc tgatagagct
     2701 agttgtttac ttttgaagaa cattgaaccc attttgacga ccttttaact ttttaatagt
     2761 ttttttttac catttacata aaagcaaaat ttacagtcta aaagttgtat attcaatacg
     2821 ttctggtacg taaaagggtt atttaatgca ccatttttac aactttagat agtatacttt
     2881 ttacttgagt gggagccata tcacaaaaat gaaatgaaag gttaaaaaag tatctaaaag
     2941 taagtttcaa ttaatattct gaaaaataat tgcatttcat gtacgtttct ctgaaatacc
     3001 cctaattcga tactatttat agatctggaa gatatatagc taggaaatag aattatatat
     3061 ttgcctagtt tgaacacctt gtgaacattt tgtttaaaaa aaaagcgtat ggtggtacta
     3121 gaaaacttta agatacagat aaataacttc actataccga ggttttggtg tttcttgaga
     3181 ctgaaggaca atcttcaaaa tggtcttaat tcttgagata gaaaagctga agttctcatg
     3241 ctgaatagtg atcagtacga ttttatctga cagattgctt tcctagctga tcataccgat
     3301 ctcggatggg tatctgctct aatccgagat agcaggtagt agaactgctg cctcgtaacc
     3361 cctagacatg gccatttcca cgcacatgta ggcgatcctg cactcgtcgg tctctcgtct
     3421 tatctcacaa aaataaaagt cggcgtttgg atggattgct cggctggctg ttggcacatt
     3481 cattacgttt tcgttgacgg cgcgagacgg ttgtgttgtt ttttaatcgg cgggcacatg
     3541 aggttgtcgg ttctctaatt ttgaaataaa aaacttgttt tcgctttaca ttcaaggaac
     3601 gcacctcctc ccctccatat atgtaaacga gcttcgtaat tgcaggctaa cacttatata
     3661 ggcattggta ctgagataca gcgagatacc gtgagctaca gatacatagc acttcacgat
     3721 gtctaactcg gttttggtgt ttttcgtgaa tggaaagaag gtacttaaag atactcggat
     3781 gccttgcgtg tctttcctta aagtcggccc actcttacta tatcgattcc ctttattgtt
     3841 ttattgccct aattgcgttt aactggcatt cgatggaatt tgccttcttg ctcaattgaa
     3901 tgtgtcgaat gtatatagta tttgctgtgc atatagtgat aacaattgac ccccatgtga
     3961 agcctcgttg tttgctggtg gccccttatc gcccgatcaa gatggtgcac ttgccaatcg
     4021 cgcatgggag cagtgctact cgaatgtggg tgagctggga tcgtcatggg catttgtgtc
     4081 tgccagatag ctcaggccct ctaatccgca cattcccacc ggctgggcgg tgtttacaat
     4141 ccagtgagct cggctggatt attcagataa agctggccat atttagcatc tggtgttggt
     4201 ggagtgcaat tggagttctt tactttgtgc tcacagcttg cgcacaagtg aagtaaataa
     4261 taataaataa ccagttgctc gtgcgcccgg catttgatag tgatagatag caatctctgt
     4321 ttgctcaggg ctggcagagc agataacact ttcgctgttt taagtttaaa caaagtcaca
     4381 gttttgacaa acaattgctt taaaccatat aaacttataa ttaaagcaat tataatgggt
     4441 taagtaagca cagatctagc tacaagatac aagatacaaa ttttccaatt aatggataaa
     4501 tggcattaga tacttttggg aaattgattt cgtttttttc attttattat atttatacat
     4561 ttcttattcc aggtgaccga agtgtcgcct gatccggagt gcacgctcct cacattcctg
     4621 cgcgaaaagc tgcggctgtg cggaacgaag ttgggatgtg cggaaggcgg atgcggcgcc
     4681 tgcaccgtga tggtgtcccg cctagaccgt cgggccaaca agatacgtca cctggcggtc
     4741 aacgcctgcc tgacgccagt gtgctcgatg cacggatgtg cggtgaccac tgtggagggt
     4801 atagggagca ccaaaacgcg tctgcatccg gtgcaggagc gactgcccaa ggcgcacgga
     4861 tcccagtgcg gcttctgcac gccgggcatt gtgatgtcca tgtacgcact tctgcggaac
     4921 gcggagcaac cctctatgcg agacttggag gtggcattcc aaggtaacct gtgccgctgc
     4981 accggctatc gacccattct cgagggctac aagacgttca ccaaggagtt tgcctgcgga
     5041 atgggcgaga agtgctgcaa agttagtggg aaaggatgtg gaaccgatgc ggagaccgat
     5101 gacaagctct tcgagcgcag cgaattccag cccttggatc ccagccagga acccatcttc
     5161 ccaccggaac ttcagctgag tgacgccttc gattcgcaga gtttgatctt tagttcggat
     5221 agggtgacct ggtatcgtcc caccaatctg gaggagctgc ttcagctgaa ggccaaacat
     5281 ccggctgcca agctggtcgt gggcaatacg gaagtgggcg ttgaggttaa gttcaagcac
     5341 ttcctctacc cgcacctcat caatcccacc caggtgaagg agctgctgga gatcaaagag
     5401 aaccaggatg gcatttactt cggtgcggct gtcagtttga tggagatcga tgcgcttctg
     5461 cggcagagaa tcgagctgct gccggaatcg gagaccagat tgttccagtg caccgtggat
     5521 atgcttcact actttgccgg caagcagatc cgcaacgtcg cctgtttggg tggaaacatc
     5581 atgaccggca gtcccatttc cgatatgaat cctgtgctct cggcagcagg agctcaactg
     5641 gaggtggcca gttttgtgga tggaaagctc caaaagagat cagttcacat gggaactggg
     5701 ttcttcactg gctatcgcag gaatgttatc gaagcccacg aggtgctgct gggcatccac
     5761 tttcggaaga ccactccgga ccagtatatc gttgctttta agcaggccag aagaagggat
     5821 gatgacatag ccatcgtaaa tgccgcaata aacgttcgct ttgaggaaaa atccaacatt
     5881 gtggcggaga tctcgatggc ttttggtgga atggcaccaa ccacagtgct cgctcctcga
     5941 acttcccaac tgatggttgg gcaggagtgg agccaccagc tcgtggagcg cgtggcggag
     6001 agcttgtgca cggagctgcc tttggctgcc tccgctccgg gtggcatgat cgcctatcgt
     6061 cgagctctgg tggtgagcct gttcttcaag gcctatctgg ccatttccct gaagctgagc
     6121 aagtcaggga tcacatcttc cgatgctctg ccaccggagg agcgaagtgg tgccgagaca
     6181 ttccacacac cagtactcaa aagtgcccag ctcttcgagc gcgtctgcag cgatcaaccc
     6241 atctgtgatc ccattgggag accaaaagtt catgcggctg ctttgaaaca ggccactggt
     6301 gaagctatct acacagatga cattccccgc atggatggtg aagtttatct ggcctttgtc
     6361 cttagtacca agccacgtgc caagatcacc aagctggatg ccagtgaagc tctggccctg
     6421 gacggagtgc atcagttctt ttgctacaag gacttaacgg agcacgagaa cgaagtggga
     6481 cccgtctttc atgatgagca cgtctttgcc gctggagaag tgcattgcta tggtcagata
     6541 gtgggcgcca tagctgccga taataaggcg ttggcccaaa gagccgctcg gctagtgaaa
     6601 gtggagtacg aggagctgag cccggttatc gtgaccatag agcaggccat cgagctcaag
     6661 tcctatttcc cggactaccc ccgattcgtg accaagggca atgtggagga ggctttatcc
     6721 caggcggatc acactttcga gggcacctgt cgaatgggcg gacaggagca cttctatctg
     6781 gagacccatg ctgcattggc cgtacctcgt gacagcgatg agctggaact cttttgctcc
     6841 acgcagcatc cctcggaggt gcagaagcta gtggcccatg taaccgcact tcctgcccac
     6901 cgtgtcgtct gtcgtgccaa gcgtttggga ggcggtttcg gcggcaagga gtccagaggc
     6961 atctccgtgg ccctacccgt tgccctggcc gcctatcgaa tgggtcgtcc tgtgcgctgt
     7021 atgttggatc gcgatgagga catgcttatc accggcacca ggcatccctt cctcttcaaa
     7081 tacaaagtgg gcttcaccaa ggagggtctg atcactgcct gcgacattga gtgctacaac
     7141 aatgccggtt ggtccatgga tctgtcattt tcggtaagag tggtgggttt atggaaaatc
     7201 catatgtagg tttctttatg cataccatgt ttgctcaaat atccatgtgt tgtattttcc
     7261 attgaacatc tggttagtgt gggaatttct aggaccccta tatgttatca tcctgagtgc
     7321 aaatagtttg aaacttttaa gaatgtttag gtctaatcag gaaagcaagc accaggatta
     7381 tattgacata aacaattata aataataatc taaaaattct taaaaatgat ctaatatata
     7441 aatcctatgt ttaggttctt gagcgcgcca tgttccactt tgagaattgc tacaggattc
     7501 ccaacgttcg cgtgggtgga tgggtctgca agacgaacct gccctcgaat acggccttcc
     7561 gtggatttgg aggaccacaa ggcatgtacg ccggtgagca tatcatccgg gatgtggccc
     7621 ggatagtggg tcgcgatgtg gtggatgtga tgcggctgaa cttctacaag actggagact
     7681 acacacacta ccaccagcag ctggagcact tccccatcga gcggtgtctg gaggattgct
     7741 tgaagcagtc gagatacgac gagaagcggc aggatattgc tcgattcaat cgggagaatc
     7801 gctggcggaa acgcggcatg gcggtggtgc ccaccaagta tggaatcgca tttggagtga
     7861 tgcacttgaa ccaagcggga tcgctgatca acatctatgg tgatggatcc gtgttgcttt
     7921 cgcacggagg agttgagatc ggacaaggtc tgaataccaa gatgattcag tgcgccgcca
     7981 gggctctggg gattccttcg gaactgattc acatttcgga aacggccacg gataaagtac
     8041 ccaacacttc acccacggcg gcgagtgtgg gatccgatct gaacggaatg gccgtactgg
     8101 atgcgtgtga aaagttgaac aaaagactgg cgcccatcaa ggaggcattg cctggaggca
     8161 cctggaagga gtggatcaac aaggcgtatt tcgatcgggt cagcctctcg gccacaggat
     8221 tctatgccat gcccgggatt ggatatcacc cggaaacgaa tcccaatgct cgcacctata
     8281 gctactacac gaatggcgtg ggagtcactg tggtagagat cgattgcctg actggcgacc
     8341 atcaggtgct cagcacagac atcgtgatgg acatcggctc tagcctgaat ccggctattg
     8401 acattggtca gatcgaggga gcattcatgc agggctatgg actgttcact ttggaggaac
     8461 tcatgtactc accacaaggc atgctttact ccagaggtcc gggcatgtac aagctgccag
     8521 gatttgccga cattcccggg gagttcaatg tcagcctact gaccggtgcc cccaatccac
     8581 gggcagtcta ctcttccaag gcagtgggtg aacctccgct cttcattgga tcatctgcat
     8641 tctttgccat taaggaggcc attgcagctg ctcgcgagga tcagggcttg agtggtgact
     8701 tcccactgga ggcgccttcc acatcggcac gcattcgaat tgcttgtcag gataagttca
     8761 cggaactggt aagttaccct tggattagtt tagaacatta aactaacttt tattattaat
     8821 ttattaatta tagcttgaaa tacccgaacc aggatcattt acgccatgga acattgtgcc
     8881 ttaaaattgt ttttattgtt tgtactgctt aagcatttaa aatgacgatt ttatttgtta
     8941 atttgtttat atatacatag tgaattaaat gttttaaaaa taattgagtc gtttaatgtg
     9001 taaactaagc tggagagctg tttgaacaaa tttatatacc actgattaat attaaatatt
     9061 ccttttagat taagaaaaat tcaatggtta cttttttgtt ctatctatta ttcaatagaa
     9121 tctcgtggca tgcaatgcat tctgattttc aattgaaaag tcttagtcac tatttatttt
     9181 gagtatatta atgaaagttg agcaagtttt ccgatgaatt gaaatatgag ttccgatttc
     9241 ggcgcgactg ctacccgaaa atataagctc aatcaaaagc aagcttcaaa ttattaaaaa
     9301 taaaacttta aaaataattt cgtctaatta atattatgag ttaattcaaa ccccacggac
     9361 atgctaaggg ttaatcaaca atcatatcgc tgtctcactc agactcaata cgacactcag
     9421 aatactattc ctttcactcg cacttattgc aagcatacgc cgacgggacc accttatgtt
     9481 atttcatcat gggtacccag cttttgttcc ctttagtgag ggttaattcc gagcttggcg
     9541 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     9601 ataggagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag gtaactcaca
     9661 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     9721 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9781 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9841 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9901 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9961 ctcggccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    10021 acaggactat aaagatacca ggcgttcccc cctggaagct ccctcgtgcg ctctcctgtt
    10081 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    10141 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    10201 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    10261 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    10321 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    10381 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    10441 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    10501 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10561 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    10621 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    10681 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    10741 tcagcgatct gtctatttcg ttcatccata gttgcctgac tgcccgtcgt gtagataact
    10801 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10861 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10921 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10981 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    11041 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    11101 acatgatccc ccatgttgtg aaaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    11161 agaagtaagt tggccgcagt gttatcactc atgcttatgg cagcactgca taattctctt
    11221 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    11281 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    11341 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    11401 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    11461 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    11521 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    11581 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    11641 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    11701 gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
    11761 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
    11821 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
    11881 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
    11941 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
    12001 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
    12061 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
    12121 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctacgc
    12181 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaagggg
    12241 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
    12301 aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattgga gctc
//
