LOCUS       PGSryhs3    11750 bp    DNA   circular  INV       04-FEB-2001
DEFINITION  pPGS_ry_hs_3.
ACCESSION   PGSryhs3
VERSION
KEYWORDS    .
SOURCE      fruit fly.
  ORGANISM  Drosophila melanogaster
            Eukaryota; Metazoa; Arthropoda; Tracheata; Hexapoda; Insecta;
            Pterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
            Drosophilidae; Drosophila.
REFERENCE   1  (bases 1 to 11750)
  AUTHORS   Gunnar,S. and Gunter,R.
  TITLE     Controlled expression of tagged proteins in Drosophila using a new
            modular P-element vector system
  JOURNAL   Mol Gen Genet 262 (6), 916-920 (2000)
REFERENCE   2  (bases 1 to 11750)
  AUTHORS   Gunnar,S. and Gunter,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2001) Institute of Genetics,
            Martin-Luther-University Halle, Weinbergweg 10, Halle 06120,
            Germany
FEATURES             Location/Qualifiers
     source          1..11750
                     /organism="Drosophila melanogaster"
     misc_feature    1..541
                     /function="5' P repeat"
     misc_feature    542..1004
                     /function="hsp module"
     misc_feature    1005..1079
                     /function="multi cloning site"
     misc_feature    1080..1390
                     /function="3' polyA module"
     misc_feature    1391..8682
                     /function="rosy module"
     misc_feature    8683..8887
                     /function="3' P repeat"
     misc_feature    8888..11750
                     /function="pBluescript II"
BASE COUNT     3086 a   2680 c   2876 g   3108 t
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gaaagccgca gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 atctagaaga atcccaaaac aaactggtta ttgtggtagg tcatttgttt ggcagaagaa
      601 aactcgagaa atttctctgg ccgttattcg ttattctctc ttttcttttt gggtctccct
      661 ctctgcacta atgctctctc actctgtcac acagtaaacg gcatactgct ctcgttggtt
      721 cgagagagcg cgcctcgaat gttcgcgaaa agagcgccgg agtataaata gagcgcttcg
      781 tctacggagc gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta
      841 agcaaataaa caagcgcagc tgaacaagct aaacaatctg cagtaaagtg caagttaaag
      901 tgaatcaatt aaaagtaacc agcaaccaag taaatcaact gcaactactg aaatctgcca
      961 agaagtaatt attgaataca agaagagaac tctgaatagg gcccatagcg gccgcctcga
     1021 ggcagaattc cctgcaggat cgatggatcc ggcgcgccgt cgacgacgtc gctagcatac
     1081 ctaggtagtt taaattgatt gttgcattcg taaaccgaaa taaatgcctg tactagaact
     1141 tccctttaaa cctattgtgc actagttagt ggtctcacct ggatctccca aaataacctc
     1201 aactaatgtc tgtcaaaaca catctacctc cctttccagg tgtcatccag ctgaaggcgc
     1261 tcttcgaggg caaaaccacg ggtgccagta tccagtacac cacgacgcaa cgaggcaggg
     1321 tgatgctggt ctacgagggt tatcggtatg tcgtgaatcg ccagagcctg aagaacgtct
     1381 tctgcctagg aagctttaac tatgtttaat ctcccataga gcttcgttat cttcggacgc
     1441 gatgtggcgc agatcccgtg ctttcgtaac agttttctct acggaatcag cggagggatc
     1501 ggcatcggct tgctgacttt tctgggcacc tcgcgaaccc acctgtccac ccacgtgggc
     1561 ttcggctcct tcttctgcgg caccatcgcc tactggatga cctgcaggta agtgactgtg
     1621 attgaaaccg tgcgtcgccc ttatcactta attatcaatg tggcatccgc aggtatcaat
     1681 ggtccgtcag gagattcgag cagcagcaat tgcgtgaggc gatgagacga caagcccttt
     1741 atgagggcac ccaaagggag agagacttag atctgaagtc ggcgtagcat aagtcagatc
     1801 cttcattact acttcgatgt ttgttttcac tctaggatac caagtaatcc tggcattgtc
     1861 aaatcccttg tgttgtaaac taccttttgt taccaaactt tatagtagcc aatatattat
     1921 atatatgttg taagtgaaat catagcttgg agtttgcttg ccactcgtag ggaattagtt
     1981 atgccaccat ctttgatttg ggattgacag aaatcaatcc atcattttaa tgattgtggt
     2041 tcctaaaacc tgttgggctt tattgtgctt tcgatttgat ttaagctgat agagctagtt
     2101 gtttactttt gaagaacatt gaacccattt tgacgacctt ttaacttttt aatagttttt
     2161 ttttaccatt tacataaaag caaaatttac agtctaaaag ttgtatattc aatacgttct
     2221 ggtacgtaaa agggttattt aatgcaccat ttttacaact ttagatagta tactttttac
     2281 ttgagtggga gccatatcac aaaaatgaaa tgaaaggtta aaaaagtatc taaaagtaag
     2341 tttcaattaa tattctgaaa aataattgca tttcatgtac gtttctctga aataccccta
     2401 attcgatact atttatagat ctggaagata tatagctagg aaatagaatt atatatttgc
     2461 ctagtttgaa caccttgtga acattttgtt taaaaaaaaa gcgtatggtg gtactagaaa
     2521 actttaagat acagataaat aacttcacta taccgaggtt ttggtgtttc ttgagactga
     2581 aggacaatct tcaaaatggt cttaattctt gagatagaaa agctgaagtt ctcatgctga
     2641 atagtgatca gtacgatttt atctgacaga ttgctttcct agctgatcat accgatctcg
     2701 gatgggtatc tgctctaatc cgagatagca ggtagtagaa ctgctgcctc gtaaccccta
     2761 gacatggcca tttccacgca catgtaggcg atcctgcact cgtcggtctc tcgtcttatc
     2821 tcacaaaaat aaaagtcggc gtttggatgg attgctcggc tggctgttgg cacattcatt
     2881 acgttttcgt tgacggcgcg agacggttgt gttgtttttt aatcggcggg cacatgaggt
     2941 tgtcggttct ctaattttga aataaaaaac ttgttttcgc tttacattca aggaacgcac
     3001 ctcctcccct ccatatatgt aaacgagctt cgtaattgca ggctaacact tatataggca
     3061 ttggtactga gatacagcga gataccgtga gctacagata catagcactt cacgatgtct
     3121 aactcggttt tggtgttttt cgtgaatgga aagaaggtac ttaaagatac tcggatgcct
     3181 tgcgtgtctt tccttaaagt cggcccactc ttactatatc gattcccttt attgttttat
     3241 tgccctaatt gcgtttaact ggcattcgat ggaatttgcc ttcttgctca attgaatgtg
     3301 tcgaatgtat atagtatttg ctgtgcatat agtgataaca attgaccccc atgtgaagcc
     3361 tcgttgtttg ctggtggccc cttatcgccc gatcaagatg gtgcacttgc caatcgcgca
     3421 tgggagcagt gctactcgaa tgtgggtgag ctgggatcgt catgggcatt tgtgtctgcc
     3481 agatagctca ggccctctaa tccgcacatt cccaccggct gggcggtgtt tacaatccag
     3541 tgagctcggc tggattattc agataaagct ggccatattt agcatctggt gttggtggag
     3601 tgcaattgga gttctttact ttgtgctcac agcttgcgca caagtgaagt aaataataat
     3661 aaataaccag ttgctcgtgc gcccggcatt tgatagtgat agatagcaat ctctgtttgc
     3721 tcagggctgg cagagcagat aacactttcg ctgttttaag tttaaacaaa gtcacagttt
     3781 tgacaaacaa ttgctttaaa ccatataaac ttataattaa agcaattata atgggttaag
     3841 taagcacaga tctagctaca agatacaaga tacaaatttt ccaattaatg gataaatggc
     3901 attagatact tttgggaaat tgatttcgtt tttttcattt tattatattt atacatttct
     3961 tattccaggt gaccgaagtg tcgcctgatc cggagtgcac gctcctcaca ttcctgcgcg
     4021 aaaagctgcg gctgtgcgga acgaagttgg gatgtgcgga aggcggatgc ggcgcctgca
     4081 ccgtgatggt gtcccgccta gaccgtcggg ccaacaagat acgtcacctg gcggtcaacg
     4141 cctgcctgac gccagtgtgc tcgatgcacg gatgtgcggt gaccactgtg gagggtatag
     4201 ggagcaccaa aacgcgtctg catccggtgc aggagcgact gcccaaggcg cacggatccc
     4261 agtgcggctt ctgcacgccg ggcattgtga tgtccatgta cgcacttctg cggaacgcgg
     4321 agcaaccctc tatgcgagac ttggaggtgg cattccaagg taacctgtgc cgctgcaccg
     4381 gctatcgacc cattctcgag ggctacaaga cgttcaccaa ggagtttgcc tgcggaatgg
     4441 gcgagaagtg ctgcaaagtt agtgggaaag gatgtggaac cgatgcggag accgatgaca
     4501 agctcttcga gcgcagcgaa ttccagccct tggatcccag ccaggaaccc atcttcccac
     4561 cggaacttca gctgagtgac gccttcgatt cgcagagttt gatctttagt tcggataggg
     4621 tgacctggta tcgtcccacc aatctggagg agctgcttca gctgaaggcc aaacatccgg
     4681 ctgccaagct ggtcgtgggc aatacggaag tgggcgttga ggttaagttc aagcacttcc
     4741 tctacccgca cctcatcaat cccacccagg tgaaggagct gctggagatc aaagagaacc
     4801 aggatggcat ttacttcggt gcggctgtca gtttgatgga gatcgatgcg cttctgcggc
     4861 agagaatcga gctgctgccg gaatcggaga ccagattgtt ccagtgcacc gtggatatgc
     4921 ttcactactt tgccggcaag cagatccgca acgtcgcctg tttgggtgga aacatcatga
     4981 ccggcagtcc catttccgat atgaatcctg tgctctcggc agcaggagct caactggagg
     5041 tggccagttt tgtggatgga aagctccaaa agagatcagt tcacatggga actgggttct
     5101 tcactggcta tcgcaggaat gttatcgaag cccacgaggt gctgctgggc atccactttc
     5161 ggaagaccac tccggaccag tatatcgttg cttttaagca ggccagaaga agggatgatg
     5221 acatagccat cgtaaatgcc gcaataaacg ttcgctttga ggaaaaatcc aacattgtgg
     5281 cggagatctc gatggctttt ggtggaatgg caccaaccac agtgctcgct cctcgaactt
     5341 cccaactgat ggttgggcag gagtggagcc accagctcgt ggagcgcgtg gcggagagct
     5401 tgtgcacgga gctgcctttg gctgcctccg ctccgggtgg catgatcgcc tatcgtcgag
     5461 ctctggtggt gagcctgttc ttcaaggcct atctggccat ttccctgaag ctgagcaagt
     5521 cagggatcac atcttccgat gctctgccac cggaggagcg aagtggtgcc gagacattcc
     5581 acacaccagt actcaaaagt gcccagctct tcgagcgcgt ctgcagcgat caacccatct
     5641 gtgatcccat tgggagacca aaagttcatg cggctgcttt gaaacaggcc actggtgaag
     5701 ctatctacac agatgacatt ccccgcatgg atggtgaagt ttatctggcc tttgtcctta
     5761 gtaccaagcc acgtgccaag atcaccaagc tggatgccag tgaagctctg gccctggacg
     5821 gagtgcatca gttcttttgc tacaaggact taacggagca cgagaacgaa gtgggacccg
     5881 tctttcatga tgagcacgtc tttgccgctg gagaagtgca ttgctatggt cagatagtgg
     5941 gcgccatagc tgccgataat aaggcgttgg cccaaagagc cgctcggcta gtgaaagtgg
     6001 agtacgagga gctgagcccg gttatcgtga ccatagagca ggccatcgag ctcaagtcct
     6061 atttcccgga ctacccccga ttcgtgacca agggcaatgt ggaggaggct ttatcccagg
     6121 cggatcacac tttcgagggc acctgtcgaa tgggcggaca ggagcacttc tatctggaga
     6181 cccatgctgc attggccgta cctcgtgaca gcgatgagct ggaactcttt tgctccacgc
     6241 agcatccctc ggaggtgcag aagctagtgg cccatgtaac cgcacttcct gcccaccgtg
     6301 tcgtctgtcg tgccaagcgt ttgggaggcg gtttcggcgg caaggagtcc agaggcatct
     6361 ccgtggccct acccgttgcc ctggccgcct atcgaatggg tcgtcctgtg cgctgtatgt
     6421 tggatcgcga tgaggacatg cttatcaccg gcaccaggca tcccttcctc ttcaaataca
     6481 aagtgggctt caccaaggag ggtctgatca ctgcctgcga cattgagtgc tacaacaatg
     6541 ccggttggtc catggatctg tcattttcgg taagagtggt gggtttatgg aaaatccata
     6601 tgtaggtttc tttatgcata ccatgtttgc tcaaatatcc atgtgttgta ttttccattg
     6661 aacatctggt tagtgtggga atttctagga cccctatatg ttatcatcct gagtgcaaat
     6721 agtttgaaac ttttaagaat gtttaggtct aatcaggaaa gcaagcacca ggattatatt
     6781 gacataaaca attataaata ataatctaaa aattcttaaa aatgatctaa tatataaatc
     6841 ctatgtttag gttcttgagc gcgccatgtt ccactttgag aattgctaca ggattcccaa
     6901 cgttcgcgtg ggtggatggg tctgcaagac gaacctgccc tcgaatacgg ccttccgtgg
     6961 atttggagga ccacaaggca tgtacgccgg tgagcatatc atccgggatg tggcccggat
     7021 agtgggtcgc gatgtggtgg atgtgatgcg gctgaacttc tacaagactg gagactacac
     7081 acactaccac cagcagctgg agcacttccc catcgagcgg tgtctggagg attgcttgaa
     7141 gcagtcgaga tacgacgaga agcggcagga tattgctcga ttcaatcggg agaatcgctg
     7201 gcggaaacgc ggcatggcgg tggtgcccac caagtatgga atcgcatttg gagtgatgca
     7261 cttgaaccaa gcgggatcgc tgatcaacat ctatggtgat ggatccgtgt tgctttcgca
     7321 cggaggagtt gagatcggac aaggtctgaa taccaagatg attcagtgcg ccgccagggc
     7381 tctggggatt ccttcggaac tgattcacat ttcggaaacg gccacggata aagtacccaa
     7441 cacttcaccc acggcggcga gtgtgggatc cgatctgaac ggaatggccg tactggatgc
     7501 gtgtgaaaag ttgaacaaaa gactggcgcc catcaaggag gcattgcctg gaggcacctg
     7561 gaaggagtgg atcaacaagg cgtatttcga tcgggtcagc ctctcggcca caggattcta
     7621 tgccatgccc gggattggat atcacccgga aacgaatccc aatgctcgca cctatagcta
     7681 ctacacgaat ggcgtgggag tcactgtggt agagatcgat tgcctgactg gcgaccatca
     7741 ggtgctcagc acagacatcg tgatggacat cggctctagc ctgaatccgg ctattgacat
     7801 tggtcagatc gagggagcat tcatgcaggg ctatggactg ttcactttgg aggaactcat
     7861 gtactcacca caaggcatgc tttactccag aggtccgggc atgtacaagc tgccaggatt
     7921 tgccgacatt cccggggagt tcaatgtcag cctactgacc ggtgccccca atccacgggc
     7981 agtctactct tccaaggcag tgggtgaacc tccgctcttc attggatcat ctgcattctt
     8041 tgccattaag gaggccattg cagctgctcg cgaggatcag ggcttgagtg gtgacttccc
     8101 actggaggcg ccttccacat cggcacgcat tcgaattgct tgtcaggata agttcacgga
     8161 actggtaagt tacccttgga ttagtttaga acattaaact aacttttatt attaatttat
     8221 taattatagc ttgaaatacc cgaaccagga tcatttacgc catggaacat tgtgccttaa
     8281 aattgttttt attgtttgta ctgcttaagc atttaaaatg acgattttat ttgttaattt
     8341 gtttatatat acatagtgaa ttaaatgttt taaaaataat tgagtcgttt aatgtgtaaa
     8401 ctaagctgga gagctgtttg aacaaattta tataccactg attaatatta aatattcctt
     8461 ttagattaag aaaaattcaa tggttacttt tttgttctat ctattattca atagaatctc
     8521 gtggcatgca atgcattctg attttcaatt gaaaagtctt agtcactatt tattttgagt
     8581 atattaatga aagttgagca agttttccga tgaattgaaa tatgagttcc gatttcggcg
     8641 cgactgctac ccgaaaatat aagctcaatc aaaagcaagc ttcaaattat taaaaataaa
     8701 actttaaaaa taatttcgtc taattaatat tatgagttaa ttcaaacccc acggacatgc
     8761 taagggttaa tcaacaatca tatcgctgtc tcactcagac tcaatacgac actcagaata
     8821 ctattccttt cactcgcact tattgcaagc atacgccgac gggaccacct tatgttattt
     8881 catcatgggt acccagcttt tgttcccttt agtgagggtt aattccgagc ttggcgtaat
     8941 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatag
     9001 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgaggtaa ctcacattaa
     9061 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     9121 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     9181 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9241 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9301 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcg
     9361 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9421 gactataaag ataccaggcg ttcccccctg gaagctccct cgtgcgctct cctgttccga
     9481 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9541 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9601 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9661 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9721 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9781 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9841 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9901 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9961 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    10021 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    10081 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    10141 cgatctgtct atttcgttca tccatagttg cctgactgcc cgtcgtgtag ataactacga
    10201 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    10261 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    10321 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    10381 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    10441 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10501 gatcccccat gttgtgaaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10561 gtaagttggc cgcagtgtta tcactcatgc ttatggcagc actgcataat tctcttactg
    10621 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10681 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10741 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10801 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10861 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10921 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10981 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    11041 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa
    11101 ttgtaaacgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
    11161 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
    11221 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
    11281 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
    11341 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
    11401 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
    11461 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
    11521 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctacgcaact
    11581 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaggggggat
    11641 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
    11701 cgacggccag tgaattgtaa tacgactcac tatagggcga attggagctc
//
