LOCUS       PGSrylexA   11575 bp    DNA   circular  INV       04-FEB-2001
DEFINITION  pPGS_ry_lexA.
ACCESSION   PGSrylexA
VERSION
KEYWORDS    .
SOURCE      fruit fly.
  ORGANISM  Drosophila melanogaster
            Eukaryota; Metazoa; Arthropoda; Tracheata; Hexapoda; Insecta;
            Pterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
            Drosophilidae; Drosophila.
REFERENCE   1  (bases 1 to 11575)
  AUTHORS   Gunnar,S. and Gunter,R.
  TITLE     Controlled expression of tagged proteins in Drosophila using a new
            modular P-element vector system
  JOURNAL   Mol Gen Genet 262 (6), 916-920 (2000)
REFERENCE   2  (bases 1 to 11575)
  AUTHORS   Gunnar,S. and Gunter,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2001) Institute of Genetics,
            Martin-Luther-University Halle, Weinbergweg 10, Halle 06120,
            Germany
FEATURES             Location/Qualifiers
     source          1..11575
                     /organism="Drosophila melanogaster"
     misc_feature    1..541
                     /function="5' P repeat"
     misc_feature    join(542..574,1195..1215)
                     /function="multi cloning site"
     misc_feature    575..1194
                     /function="lexA module"
     misc_feature    1216..8507
                     /function="rosy module"
     misc_feature    8508..8712
                     /function="3' P repeat"
     misc_feature    8713..11575
                     /function="pBluescript II"
BASE COUNT     2996 a   2632 c   2891 g   3056 t
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gaaagccgca gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 atctagaact agtccgcgga gcgctgggcc catagcggcc gcatgaaagc gttaacggcc
      601 aggcaacaag aggtgtttga tctcatccgt gatcacatca gccagacagg tatgccgccg
      661 acgcgtgcgg aaatcgcgca gcgtttgggg ttccgttccc caaacgcggc tgaagaacat
      721 ctgaaggcgc tggcacgcaa aggcgttatt gaaattgttt ccggcgcatc acgcgggatt
      781 cgtctgttgc aggaagagga agaagggttg ccgctggtag gtcgtgtggc tgccggtgaa
      841 ccacttctgg cgcaacagca tattgaaggt cattatcagg tcgatccttc cttattcaag
      901 ccgaatgctg atttcctgct gcgcgtcagc gggatgtcga tgaaagatat cggcattatg
      961 gatggtgact tgctggcagt gcataaaact caggatgtac gtaacggtca ggtcgttgtc
     1021 gcacgtattg atgacgaagt taccgttaag cgcctgaaaa aacagggcaa taaagtcgaa
     1081 ctgttgccag aaaatagcga gtttaaacca attgtcgtag atcttcgtca gcagagcttc
     1141 accattgaag ggctggcggt tggggttatt cgcaacggcg actggctggt cgacgacgtc
     1201 gctagcatac ctaggaagct ttaactatgt ttaatctccc atagagcttc gttatcttcg
     1261 gacgcgatgt ggcgcagatc ccgtgctttc gtaacagttt tctctacgga atcagcggag
     1321 ggatcggcat cggcttgctg acttttctgg gcacctcgcg aacccacctg tccacccacg
     1381 tgggcttcgg ctccttcttc tgcggcacca tcgcctactg gatgacctgc aggtaagtga
     1441 ctgtgattga aaccgtgcgt cgcccttatc acttaattat caatgtggca tccgcaggta
     1501 tcaatggtcc gtcaggagat tcgagcagca gcaattgcgt gaggcgatga gacgacaagc
     1561 cctttatgag ggcacccaaa gggagagaga cttagatctg aagtcggcgt agcataagtc
     1621 agatccttca ttactacttc gatgtttgtt ttcactctag gataccaagt aatcctggca
     1681 ttgtcaaatc ccttgtgttg taaactacct tttgttacca aactttatag tagccaatat
     1741 attatatata tgttgtaagt gaaatcatag cttggagttt gcttgccact cgtagggaat
     1801 tagttatgcc accatctttg atttgggatt gacagaaatc aatccatcat tttaatgatt
     1861 gtggttccta aaacctgttg ggctttattg tgctttcgat ttgatttaag ctgatagagc
     1921 tagttgttta cttttgaaga acattgaacc cattttgacg accttttaac tttttaatag
     1981 ttttttttta ccatttacat aaaagcaaaa tttacagtct aaaagttgta tattcaatac
     2041 gttctggtac gtaaaagggt tatttaatgc accattttta caactttaga tagtatactt
     2101 tttacttgag tgggagccat atcacaaaaa tgaaatgaaa ggttaaaaaa gtatctaaaa
     2161 gtaagtttca attaatattc tgaaaaataa ttgcatttca tgtacgtttc tctgaaatac
     2221 ccctaattcg atactattta tagatctgga agatatatag ctaggaaata gaattatata
     2281 tttgcctagt ttgaacacct tgtgaacatt ttgtttaaaa aaaaagcgta tggtggtact
     2341 agaaaacttt aagatacaga taaataactt cactataccg aggttttggt gtttcttgag
     2401 actgaaggac aatcttcaaa atggtcttaa ttcttgagat agaaaagctg aagttctcat
     2461 gctgaatagt gatcagtacg attttatctg acagattgct ttcctagctg atcataccga
     2521 tctcggatgg gtatctgctc taatccgaga tagcaggtag tagaactgct gcctcgtaac
     2581 ccctagacat ggccatttcc acgcacatgt aggcgatcct gcactcgtcg gtctctcgtc
     2641 ttatctcaca aaaataaaag tcggcgtttg gatggattgc tcggctggct gttggcacat
     2701 tcattacgtt ttcgttgacg gcgcgagacg gttgtgttgt tttttaatcg gcgggcacat
     2761 gaggttgtcg gttctctaat tttgaaataa aaaacttgtt ttcgctttac attcaaggaa
     2821 cgcacctcct cccctccata tatgtaaacg agcttcgtaa ttgcaggcta acacttatat
     2881 aggcattggt actgagatac agcgagatac cgtgagctac agatacatag cacttcacga
     2941 tgtctaactc ggttttggtg tttttcgtga atggaaagaa ggtacttaaa gatactcgga
     3001 tgccttgcgt gtctttcctt aaagtcggcc cactcttact atatcgattc cctttattgt
     3061 tttattgccc taattgcgtt taactggcat tcgatggaat ttgccttctt gctcaattga
     3121 atgtgtcgaa tgtatatagt atttgctgtg catatagtga taacaattga cccccatgtg
     3181 aagcctcgtt gtttgctggt ggccccttat cgcccgatca agatggtgca cttgccaatc
     3241 gcgcatggga gcagtgctac tcgaatgtgg gtgagctggg atcgtcatgg gcatttgtgt
     3301 ctgccagata gctcaggccc tctaatccgc acattcccac cggctgggcg gtgtttacaa
     3361 tccagtgagc tcggctggat tattcagata aagctggcca tatttagcat ctggtgttgg
     3421 tggagtgcaa ttggagttct ttactttgtg ctcacagctt gcgcacaagt gaagtaaata
     3481 ataataaata accagttgct cgtgcgcccg gcatttgata gtgatagata gcaatctctg
     3541 tttgctcagg gctggcagag cagataacac tttcgctgtt ttaagtttaa acaaagtcac
     3601 agttttgaca aacaattgct ttaaaccata taaacttata attaaagcaa ttataatggg
     3661 ttaagtaagc acagatctag ctacaagata caagatacaa attttccaat taatggataa
     3721 atggcattag atacttttgg gaaattgatt tcgttttttt cattttatta tatttataca
     3781 tttcttattc caggtgaccg aagtgtcgcc tgatccggag tgcacgctcc tcacattcct
     3841 gcgcgaaaag ctgcggctgt gcggaacgaa gttgggatgt gcggaaggcg gatgcggcgc
     3901 ctgcaccgtg atggtgtccc gcctagaccg tcgggccaac aagatacgtc acctggcggt
     3961 caacgcctgc ctgacgccag tgtgctcgat gcacggatgt gcggtgacca ctgtggaggg
     4021 tatagggagc accaaaacgc gtctgcatcc ggtgcaggag cgactgccca aggcgcacgg
     4081 atcccagtgc ggcttctgca cgccgggcat tgtgatgtcc atgtacgcac ttctgcggaa
     4141 cgcggagcaa ccctctatgc gagacttgga ggtggcattc caaggtaacc tgtgccgctg
     4201 caccggctat cgacccattc tcgagggcta caagacgttc accaaggagt ttgcctgcgg
     4261 aatgggcgag aagtgctgca aagttagtgg gaaaggatgt ggaaccgatg cggagaccga
     4321 tgacaagctc ttcgagcgca gcgaattcca gcccttggat cccagccagg aacccatctt
     4381 cccaccggaa cttcagctga gtgacgcctt cgattcgcag agtttgatct ttagttcgga
     4441 tagggtgacc tggtatcgtc ccaccaatct ggaggagctg cttcagctga aggccaaaca
     4501 tccggctgcc aagctggtcg tgggcaatac ggaagtgggc gttgaggtta agttcaagca
     4561 cttcctctac ccgcacctca tcaatcccac ccaggtgaag gagctgctgg agatcaaaga
     4621 gaaccaggat ggcatttact tcggtgcggc tgtcagtttg atggagatcg atgcgcttct
     4681 gcggcagaga atcgagctgc tgccggaatc ggagaccaga ttgttccagt gcaccgtgga
     4741 tatgcttcac tactttgccg gcaagcagat ccgcaacgtc gcctgtttgg gtggaaacat
     4801 catgaccggc agtcccattt ccgatatgaa tcctgtgctc tcggcagcag gagctcaact
     4861 ggaggtggcc agttttgtgg atggaaagct ccaaaagaga tcagttcaca tgggaactgg
     4921 gttcttcact ggctatcgca ggaatgttat cgaagcccac gaggtgctgc tgggcatcca
     4981 ctttcggaag accactccgg accagtatat cgttgctttt aagcaggcca gaagaaggga
     5041 tgatgacata gccatcgtaa atgccgcaat aaacgttcgc tttgaggaaa aatccaacat
     5101 tgtggcggag atctcgatgg cttttggtgg aatggcacca accacagtgc tcgctcctcg
     5161 aacttcccaa ctgatggttg ggcaggagtg gagccaccag ctcgtggagc gcgtggcgga
     5221 gagcttgtgc acggagctgc ctttggctgc ctccgctccg ggtggcatga tcgcctatcg
     5281 tcgagctctg gtggtgagcc tgttcttcaa ggcctatctg gccatttccc tgaagctgag
     5341 caagtcaggg atcacatctt ccgatgctct gccaccggag gagcgaagtg gtgccgagac
     5401 attccacaca ccagtactca aaagtgccca gctcttcgag cgcgtctgca gcgatcaacc
     5461 catctgtgat cccattggga gaccaaaagt tcatgcggct gctttgaaac aggccactgg
     5521 tgaagctatc tacacagatg acattccccg catggatggt gaagtttatc tggcctttgt
     5581 ccttagtacc aagccacgtg ccaagatcac caagctggat gccagtgaag ctctggccct
     5641 ggacggagtg catcagttct tttgctacaa ggacttaacg gagcacgaga acgaagtggg
     5701 acccgtcttt catgatgagc acgtctttgc cgctggagaa gtgcattgct atggtcagat
     5761 agtgggcgcc atagctgccg ataataaggc gttggcccaa agagccgctc ggctagtgaa
     5821 agtggagtac gaggagctga gcccggttat cgtgaccata gagcaggcca tcgagctcaa
     5881 gtcctatttc ccggactacc cccgattcgt gaccaagggc aatgtggagg aggctttatc
     5941 ccaggcggat cacactttcg agggcacctg tcgaatgggc ggacaggagc acttctatct
     6001 ggagacccat gctgcattgg ccgtacctcg tgacagcgat gagctggaac tcttttgctc
     6061 cacgcagcat ccctcggagg tgcagaagct agtggcccat gtaaccgcac ttcctgccca
     6121 ccgtgtcgtc tgtcgtgcca agcgtttggg aggcggtttc ggcggcaagg agtccagagg
     6181 catctccgtg gccctacccg ttgccctggc cgcctatcga atgggtcgtc ctgtgcgctg
     6241 tatgttggat cgcgatgagg acatgcttat caccggcacc aggcatccct tcctcttcaa
     6301 atacaaagtg ggcttcacca aggagggtct gatcactgcc tgcgacattg agtgctacaa
     6361 caatgccggt tggtccatgg atctgtcatt ttcggtaaga gtggtgggtt tatggaaaat
     6421 ccatatgtag gtttctttat gcataccatg tttgctcaaa tatccatgtg ttgtattttc
     6481 cattgaacat ctggttagtg tgggaatttc taggacccct atatgttatc atcctgagtg
     6541 caaatagttt gaaactttta agaatgttta ggtctaatca ggaaagcaag caccaggatt
     6601 atattgacat aaacaattat aaataataat ctaaaaattc ttaaaaatga tctaatatat
     6661 aaatcctatg tttaggttct tgagcgcgcc atgttccact ttgagaattg ctacaggatt
     6721 cccaacgttc gcgtgggtgg atgggtctgc aagacgaacc tgccctcgaa tacggccttc
     6781 cgtggatttg gaggaccaca aggcatgtac gccggtgagc atatcatccg ggatgtggcc
     6841 cggatagtgg gtcgcgatgt ggtggatgtg atgcggctga acttctacaa gactggagac
     6901 tacacacact accaccagca gctggagcac ttccccatcg agcggtgtct ggaggattgc
     6961 ttgaagcagt cgagatacga cgagaagcgg caggatattg ctcgattcaa tcgggagaat
     7021 cgctggcgga aacgcggcat ggcggtggtg cccaccaagt atggaatcgc atttggagtg
     7081 atgcacttga accaagcggg atcgctgatc aacatctatg gtgatggatc cgtgttgctt
     7141 tcgcacggag gagttgagat cggacaaggt ctgaatacca agatgattca gtgcgccgcc
     7201 agggctctgg ggattccttc ggaactgatt cacatttcgg aaacggccac ggataaagta
     7261 cccaacactt cacccacggc ggcgagtgtg ggatccgatc tgaacggaat ggccgtactg
     7321 gatgcgtgtg aaaagttgaa caaaagactg gcgcccatca aggaggcatt gcctggaggc
     7381 acctggaagg agtggatcaa caaggcgtat ttcgatcggg tcagcctctc ggccacagga
     7441 ttctatgcca tgcccgggat tggatatcac ccggaaacga atcccaatgc tcgcacctat
     7501 agctactaca cgaatggcgt gggagtcact gtggtagaga tcgattgcct gactggcgac
     7561 catcaggtgc tcagcacaga catcgtgatg gacatcggct ctagcctgaa tccggctatt
     7621 gacattggtc agatcgaggg agcattcatg cagggctatg gactgttcac tttggaggaa
     7681 ctcatgtact caccacaagg catgctttac tccagaggtc cgggcatgta caagctgcca
     7741 ggatttgccg acattcccgg ggagttcaat gtcagcctac tgaccggtgc ccccaatcca
     7801 cgggcagtct actcttccaa ggcagtgggt gaacctccgc tcttcattgg atcatctgca
     7861 ttctttgcca ttaaggaggc cattgcagct gctcgcgagg atcagggctt gagtggtgac
     7921 ttcccactgg aggcgccttc cacatcggca cgcattcgaa ttgcttgtca ggataagttc
     7981 acggaactgg taagttaccc ttggattagt ttagaacatt aaactaactt ttattattaa
     8041 tttattaatt atagcttgaa atacccgaac caggatcatt tacgccatgg aacattgtgc
     8101 cttaaaattg tttttattgt ttgtactgct taagcattta aaatgacgat tttatttgtt
     8161 aatttgttta tatatacata gtgaattaaa tgttttaaaa ataattgagt cgtttaatgt
     8221 gtaaactaag ctggagagct gtttgaacaa atttatatac cactgattaa tattaaatat
     8281 tccttttaga ttaagaaaaa ttcaatggtt acttttttgt tctatctatt attcaataga
     8341 atctcgtggc atgcaatgca ttctgatttt caattgaaaa gtcttagtca ctatttattt
     8401 tgagtatatt aatgaaagtt gagcaagttt tccgatgaat tgaaatatga gttccgattt
     8461 cggcgcgact gctacccgaa aatataagct caatcaaaag caagcttcaa attattaaaa
     8521 ataaaacttt aaaaataatt tcgtctaatt aatattatga gttaattcaa accccacgga
     8581 catgctaagg gttaatcaac aatcatatcg ctgtctcact cagactcaat acgacactca
     8641 gaatactatt cctttcactc gcacttattg caagcatacg ccgacgggac caccttatgt
     8701 tatttcatca tgggtaccca gcttttgttc cctttagtga gggttaattc cgagcttggc
     8761 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     8821 cataggagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga ggtaactcac
     8881 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8941 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     9001 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     9061 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     9121 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     9181 gctcggcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     9241 gacaggacta taaagatacc aggcgttccc ccctggaagc tccctcgtgc gctctcctgt
     9301 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     9361 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     9421 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9481 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9541 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9601 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9661 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9721 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9781 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9841 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9901 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9961 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctgcccgtcg tgtagataac
    10021 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    10081 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    10141 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    10201 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    10261 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    10321 tacatgatcc cccatgttgt gaaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    10381 cagaagtaag ttggccgcag tgttatcact catgcttatg gcagcactgc ataattctct
    10441 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    10501 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    10561 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10621 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10681 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    10741 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10801 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10861 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10921 tgaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
    10981 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
    11041 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
    11101 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
    11161 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
    11221 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
    11281 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
    11341 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctacg
    11401 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaggg
    11461 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    11521 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg agctc
//
