LOCUS       PGSvEGFP     6298 bp    DNA   circular  INV       04-FEB-2001
DEFINITION  pPGS_v_EGFP.
ACCESSION   PGSvEGFP
VERSION
KEYWORDS    .
SOURCE      fruit fly.
  ORGANISM  Drosophila melanogaster
            Eukaryota; Metazoa; Arthropoda; Tracheata; Hexapoda; Insecta;
            Pterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
            Drosophilidae; Drosophila.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Gunnar,S. and Gunter,R.
  TITLE     Controlled expression of tagged proteins in Drosophila using a new
            modular P-element vector system
  JOURNAL   Mol Gen Genet 262 (6), 916-920 (2000)
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Gunnar,S. and Gunter,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2001) Institute of Genetics,
            Martin-Luther-University Halle, Weinbergweg 10, Halle 06120,
            Germany
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Drosophila melanogaster"
     misc_feature    1..541
                     /function="5' P repeat"
     misc_feature    join(542..574,1316..1336)
                     /function="multi cloning site"
     misc_feature    575..1315
                     /function="EGFP module"
     misc_feature    1337..3230
                     /function="vermilion module"
     misc_feature    3231..3435
                     /function="3' P repeat"
     misc_feature    3436..6298
                     /function="pBluescript II"
BASE COUNT     1690 a   1569 c   1564 g   1473 t      2 others
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gaaagccgca gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 atctagaact agtccgcgga gcgctgggcc catagcggcc gcatggtgag caagggcgag
      601 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      661 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      721 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
      781 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      841 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      901 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      961 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1021 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     1081 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1141 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1201 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1261 gccgccggga tcactctcgg catggacgag ctgtacaagg cccgggcaag tcgacgacgt
     1321 cgctagcata cctaggaagc ttnatttatt ttgttatgtt atatgtatta tatgtcagac
     1381 ataaagaaaa ggaacacatc aaatgtgata acaaagacta aacaagtaat tttattacac
     1441 caaaacgaca aaacagtagg cagaacaaac aacgcatagc caaacattga ccaattggat
     1501 accctgccga ttgtcagaca cttttgttga tcagtttctt gcgaatggtc tcgtccagcg
     1561 gtggaatcgc ctcgcgggga atcagaaaag tggacagatt gaacagatcc agaaacacct
     1621 tgtaccgatc actgaaacca aaaaaaaaca aagggagaac agtttgagtt cattgatccc
     1681 cgatataatc acatctgcga tgatcacctg agagtggagc gcagatattg atatccagac
     1741 gagccaccag tgcccaactg ttgggatcca atcatgcgtt gcaccatgat cacgtgattg
     1801 tctgcggcgg gaatagaaag tatttggtta ggaaaaccag tcttaaacat aagatatatt
     1861 tataaaagag tatcaaagaa tgcaatactt acatctccac ttggttatta acgagtcgat
     1921 gtccatgagc agggtgagca actggtgtgg ttggctgaac ctgggttcat ccctatagaa
     1981 ggtgatcatg atggctccct gaagggcacg atggctaaac cggcgatccc cacgacgcac
     2041 cagtgcatcg tgcactgccg gatcaaagat ggagcgatac acctcgcgtc gcttctcaat
     2101 gtccatgagg cggtagtttt tcgccttctc cacgggctcc tccatggcgc tctgtacctg
     2161 cgcctccagg aatcgatcga cgctctcctg aaacttggcc cagaagttga agccactctc
     2221 ctccagtccg ggcgtcctct ccagccatcg ctgcactagc tccagtagcg agggatcttt
     2281 ctccgagttg cgaatcgaat tccgcgcctc ctcgtcgcta aagacatccg agtacttctg
     2341 gttgtatctc acccgctgct ctgtcagaac tcccagcttg ttctcgatca aacggaactg
     2401 cagcgactga aaaccagatg cgggtgccag gtacttgcgg aagtccatga agtctagcgg
     2461 ggtcatggtc tccagaatgg gcacttggtc caccaggagc tgtacaaagg aagttataaa
     2521 cggattttgg taagagattc agaaagcact cacttttaga atcagaacca ctcggttcag
     2581 tcgcttgaca atctccagcg tcttggtttc atcgatgacc tctgcatcca acatgtctcg
     2641 tatggagtcg aactcaaaga tgatctgctt gaaccaaagc tcgtaggctg tggcgaaggt
     2701 acttaaatgc cattgagtgt tgtcatcaaa gttgtaaacc tactcaccct ggtgcgtgat
     2761 gatgaacaga tgctcatcgt gcacgggtcg cttgtcctcc tcggacagca tacactgggc
     2821 atccagcagt ttgtccagca tcagatactc tccatagatt ttgcccactt ccgtggttaa
     2881 tggcaccgcc gaatcatcgt gatcgtttct gtatgggttt gaattgaatc gcagaactga
     2941 agatcgattg gcattcctgg acagcacgtg ctggtgctca cccgtttcct gcatagggac
     3001 agctcatggt gcacagctca gatcagatcg tgactcctcg agcggcggat gctggcgaac
     3061 tgatctccgc cagcggaccg gagatgagac cccagcgaac cgataacaga gcgagagagc
     3121 tccagttccg actgattgca cagtcggtga tctgggcgat gggcactgcc agataggctg
     3181 ggaattatca atcacttgag gtgaaagtgc ggcgcacaca aatnaagctt caaattatta
     3241 aaaataaaac tttaaaaata atttcgtcta attaatatta tgagttaatt caaaccccac
     3301 ggacatgcta agggttaatc aacaatcata tcgctgtctc actcagactc aatacgacac
     3361 tcagaatact attcctttca ctcgcactta ttgcaagcat acgccgacgg gaccacctta
     3421 tgttatttca tcatgggtac ccagcttttg ttccctttag tgagggttaa ttccgagctt
     3481 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     3541 caacatagga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact
     3601 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     3661 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     3721 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3781 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3841 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3901 taggctcggc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3961 cccgacagga ctataaagat accaggcgtt cccccctgga agctccctcg tgcgctctcc
     4021 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4081 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4141 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4201 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4261 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4321 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4381 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4441 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4501 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4561 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     4621 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     4681 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactgcccg tcgtgtagat
     4741 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     4801 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     4861 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     4921 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     4981 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     5041 agttacatga tcccccatgt tgtgaaaaaa agcggttagc tccttcggtc ctccgatcgt
     5101 tgtcagaagt aagttggccg cagtgttatc actcatgctt atggcagcac tgcataattc
     5161 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     5221 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     5281 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     5341 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     5401 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     5461 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     5521 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     5581 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     5641 acctgaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     5701 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     5761 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     5821 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     5881 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     5941 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     6001 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     6061 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
     6121 acgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     6181 ggggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     6241 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctc
//
