LOCUS       PGSvEGFP3    6603 bp    DNA   circular  INV       04-FEB-2001
DEFINITION  pPGS_v_EGFP_3.
ACCESSION   PGSvEGFP3
VERSION
KEYWORDS    .
SOURCE      fruit fly.
  ORGANISM  Drosophila melanogaster
            Eukaryota; Metazoa; Arthropoda; Tracheata; Hexapoda; Insecta;
            Pterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
            Drosophilidae; Drosophila.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Gunnar,S. and Gunter,R.
  TITLE     Controlled expression of tagged proteins in Drosophila using a new
            modular P-element vector system
  JOURNAL   Mol Gen Genet 262 (6), 916-920 (2000)
REFERENCE   2  (bases 1 to 6603)
  AUTHORS   Gunnar,S. and Gunter,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2001) Institute of Genetics,
            Martin-Luther-University Halle, Weinbergweg 10, Halle 06120,
            Germany
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="Drosophila melanogaster"
     misc_feature    1..541
                     /function="5' P repeat"
     misc_feature    join(542..574,1316..1330)
                     /function="multi cloning site"
     misc_feature    575..1315
                     /function="EGFP module"
     misc_feature    1331..1641
                     /function="3' polyA module"
     misc_feature    1642..3535
                     /function="vermilion module"
     misc_feature    3536..3740
                     /function="3' P repeat"
     misc_feature    3741..6603
                     /function="pBluescript II"
BASE COUNT     1771 a   1647 c   1631 g   1552 t      2 others
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gaaagccgca gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 atctagaact agtccgcgga gcgctgggcc catagcggcc gcatggtgag caagggcgag
      601 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      661 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      721 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
      781 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      841 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      901 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      961 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1021 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     1081 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1141 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1201 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1261 gccgccggga tcactctcgg catggacgag ctgtacaagg cccgggcaag tcgacgacgt
     1321 cgctagcata cctaggtagt ttaaattgat tgttgcattc gtaaaccgaa ataaatgcct
     1381 gtactagaac ttccctttaa acctattgtg cactagttag tggtctcacc tggatctccc
     1441 aaaataacct caactaatgt ctgtcaaaac acatctacct ccctttccag gtgtcatcca
     1501 gctgaaggcg ctcttcgagg gcaaaaccac gggtgccagt atccagtaca ccacgacgca
     1561 acgaggcagg gtgatgctgg tctacgaggg ttatcggtat gtcgtgaatc gccagagcct
     1621 gaagaacgtc ttctgcctag gaagcttnat ttattttgtt atgttatatg tattatatgt
     1681 cagacataaa gaaaaggaac acatcaaatg tgataacaaa gactaaacaa gtaattttat
     1741 tacaccaaaa cgacaaaaca gtaggcagaa caaacaacgc atagccaaac attgaccaat
     1801 tggataccct gccgattgtc agacactttt gttgatcagt ttcttgcgaa tggtctcgtc
     1861 cagcggtgga atcgcctcgc ggggaatcag aaaagtggac agattgaaca gatccagaaa
     1921 caccttgtac cgatcactga aaccaaaaaa aaacaaaggg agaacagttt gagttcattg
     1981 atccccgata taatcacatc tgcgatgatc acctgagagt ggagcgcaga tattgatatc
     2041 cagacgagcc accagtgccc aactgttggg atccaatcat gcgttgcacc atgatcacgt
     2101 gattgtctgc ggcgggaata gaaagtattt ggttaggaaa accagtctta aacataagat
     2161 atatttataa aagagtatca aagaatgcaa tacttacatc tccacttggt tattaacgag
     2221 tcgatgtcca tgagcagggt gagcaactgg tgtggttggc tgaacctggg ttcatcccta
     2281 tagaaggtga tcatgatggc tccctgaagg gcacgatggc taaaccggcg atccccacga
     2341 cgcaccagtg catcgtgcac tgccggatca aagatggagc gatacacctc gcgtcgcttc
     2401 tcaatgtcca tgaggcggta gtttttcgcc ttctccacgg gctcctccat ggcgctctgt
     2461 acctgcgcct ccaggaatcg atcgacgctc tcctgaaact tggcccagaa gttgaagcca
     2521 ctctcctcca gtccgggcgt cctctccagc catcgctgca ctagctccag tagcgaggga
     2581 tctttctccg agttgcgaat cgaattccgc gcctcctcgt cgctaaagac atccgagtac
     2641 ttctggttgt atctcacccg ctgctctgtc agaactccca gcttgttctc gatcaaacgg
     2701 aactgcagcg actgaaaacc agatgcgggt gccaggtact tgcggaagtc catgaagtct
     2761 agcggggtca tggtctccag aatgggcact tggtccacca ggagctgtac aaaggaagtt
     2821 ataaacggat tttggtaaga gattcagaaa gcactcactt ttagaatcag aaccactcgg
     2881 ttcagtcgct tgacaatctc cagcgtcttg gtttcatcga tgacctctgc atccaacatg
     2941 tctcgtatgg agtcgaactc aaagatgatc tgcttgaacc aaagctcgta ggctgtggcg
     3001 aaggtactta aatgccattg agtgttgtca tcaaagttgt aaacctactc accctggtgc
     3061 gtgatgatga acagatgctc atcgtgcacg ggtcgcttgt cctcctcgga cagcatacac
     3121 tgggcatcca gcagtttgtc cagcatcaga tactctccat agattttgcc cacttccgtg
     3181 gttaatggca ccgccgaatc atcgtgatcg tttctgtatg ggtttgaatt gaatcgcaga
     3241 actgaagatc gattggcatt cctggacagc acgtgctggt gctcacccgt ttcctgcata
     3301 gggacagctc atggtgcaca gctcagatca gatcgtgact cctcgagcgg cggatgctgg
     3361 cgaactgatc tccgccagcg gaccggagat gagaccccag cgaaccgata acagagcgag
     3421 agagctccag ttccgactga ttgcacagtc ggtgatctgg gcgatgggca ctgccagata
     3481 ggctgggaat tatcaatcac ttgaggtgaa agtgcggcgc acacaaatna agcttcaaat
     3541 tattaaaaat aaaactttaa aaataatttc gtctaattaa tattatgagt taattcaaac
     3601 cccacggaca tgctaagggt taatcaacaa tcatatcgct gtctcactca gactcaatac
     3661 gacactcaga atactattcc tttcactcgc acttattgca agcatacgcc gacgggacca
     3721 ccttatgtta tttcatcatg ggtacccagc ttttgttccc tttagtgagg gttaattccg
     3781 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     3841 ccacacaaca taggagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagg
     3901 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     3961 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     4021 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4081 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     4141 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4201 ttccataggc tcggcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4261 cgaaacccga caggactata aagataccag gcgttccccc ctggaagctc cctcgtgcgc
     4321 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4381 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4441 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4501 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4561 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4621 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4681 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4741 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4801 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4861 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     4921 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     4981 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact gcccgtcgtg
     5041 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     5101 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     5161 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     5221 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     5281 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     5341 aggcgagtta catgatcccc catgttgtga aaaaaagcgg ttagctcctt cggtcctccg
     5401 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tgcttatggc agcactgcat
     5461 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     5521 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     5581 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     5641 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     5701 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     5761 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     5821 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5881 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5941 gtgccacctg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     6001 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     6061 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     6121 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     6181 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     6241 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     6301 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     6361 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     6421 aggctacgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     6481 gcgaaggggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     6541 cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg cgaattggag
     6601 ctc
//
