LOCUS       PGSvUEGFP3   6956 bp    DNA   circular  INV       04-FEB-2001
DEFINITION  pPGS_v_UAS_EGFP_3.
ACCESSION   PGSvUEGFP3
VERSION
KEYWORDS    .
SOURCE      fruit fly.
  ORGANISM  Drosophila melanogaster
            Eukaryota; Metazoa; Arthropoda; Tracheata; Hexapoda; Insecta;
            Pterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
            Drosophilidae; Drosophila.
REFERENCE   1  (bases 1 to 6956)
  AUTHORS   Gunnar,S. and Gunter,R.
  TITLE     Controlled expression of tagged proteins in Drosophila using a new
            modular P-element vector system
  JOURNAL   Mol Gen Genet 262 (6), 916-920 (2000)
REFERENCE   2  (bases 1 to 6956)
  AUTHORS   Gunnar,S. and Gunter,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2001) Institute of Genetics,
            Martin-Luther-University Halle, Weinbergweg 10, Halle 06120,
            Germany
FEATURES             Location/Qualifiers
     source          1..6956
                     /organism="Drosophila melanogaster"
     misc_feature    1..541
                     /function="5' P repeat"
     misc_feature    542..924
                     /function="UAS module"
     misc_feature    join(925..927,1669..1683)
                     /function="multi cloning site"
     misc_feature    928..1668
                     /function="EGFP module"
     misc_feature    1684..1994
                     /function="3' polyA module"
     misc_feature    1995..3888
                     /function="vermilion module"
     misc_feature    3889..4093
                     /function="3' P repeat"
     misc_feature    4094..6956
                     /function="pBluescript II"
BASE COUNT     1894 a   1727 c   1713 g   1620 t      2 others
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gaaagccgca gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 atctagatgc atgcctgcag gtcggagtac tgtcctccga gcggagtact gtcctccgag
      601 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
      661 agactctagc gagcgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa
      721 ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc
      781 tgaacaagct aaacaatctg cagtaaagtg caagttaaag tgaatcaatt aaaagtaacc
      841 agcaaccaag taaatcaact gcaactactg aaatctgcca agaagtaatt attgaataca
      901 agaagagaac tctgaatagg gcccatagcg gccgcatggt gagcaagggc gaggagctgt
      961 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     1021 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     1081 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     1141 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     1201 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     1261 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     1321 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     1381 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     1441 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     1501 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     1561 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     1621 ggatcactct cggcatggac gagctgtaca aggcccgggc aagtcgacga cgtcgctagc
     1681 atacctaggt agtttaaatt gattgttgca ttcgtaaacc gaaataaatg cctgtactag
     1741 aacttccctt taaacctatt gtgcactagt tagtggtctc acctggatct cccaaaataa
     1801 cctcaactaa tgtctgtcaa aacacatcta cctccctttc caggtgtcat ccagctgaag
     1861 gcgctcttcg agggcaaaac cacgggtgcc agtatccagt acaccacgac gcaacgaggc
     1921 agggtgatgc tggtctacga gggttatcgg tatgtcgtga atcgccagag cctgaagaac
     1981 gtcttctgcc taggaagctt natttatttt gttatgttat atgtattata tgtcagacat
     2041 aaagaaaagg aacacatcaa atgtgataac aaagactaaa caagtaattt tattacacca
     2101 aaacgacaaa acagtaggca gaacaaacaa cgcatagcca aacattgacc aattggatac
     2161 cctgccgatt gtcagacact tttgttgatc agtttcttgc gaatggtctc gtccagcggt
     2221 ggaatcgcct cgcggggaat cagaaaagtg gacagattga acagatccag aaacaccttg
     2281 taccgatcac tgaaaccaaa aaaaaacaaa gggagaacag tttgagttca ttgatccccg
     2341 atataatcac atctgcgatg atcacctgag agtggagcgc agatattgat atccagacga
     2401 gccaccagtg cccaactgtt gggatccaat catgcgttgc accatgatca cgtgattgtc
     2461 tgcggcggga atagaaagta tttggttagg aaaaccagtc ttaaacataa gatatattta
     2521 taaaagagta tcaaagaatg caatacttac atctccactt ggttattaac gagtcgatgt
     2581 ccatgagcag ggtgagcaac tggtgtggtt ggctgaacct gggttcatcc ctatagaagg
     2641 tgatcatgat ggctccctga agggcacgat ggctaaaccg gcgatcccca cgacgcacca
     2701 gtgcatcgtg cactgccgga tcaaagatgg agcgatacac ctcgcgtcgc ttctcaatgt
     2761 ccatgaggcg gtagtttttc gccttctcca cgggctcctc catggcgctc tgtacctgcg
     2821 cctccaggaa tcgatcgacg ctctcctgaa acttggccca gaagttgaag ccactctcct
     2881 ccagtccggg cgtcctctcc agccatcgct gcactagctc cagtagcgag ggatctttct
     2941 ccgagttgcg aatcgaattc cgcgcctcct cgtcgctaaa gacatccgag tacttctggt
     3001 tgtatctcac ccgctgctct gtcagaactc ccagcttgtt ctcgatcaaa cggaactgca
     3061 gcgactgaaa accagatgcg ggtgccaggt acttgcggaa gtccatgaag tctagcgggg
     3121 tcatggtctc cagaatgggc acttggtcca ccaggagctg tacaaaggaa gttataaacg
     3181 gattttggta agagattcag aaagcactca cttttagaat cagaaccact cggttcagtc
     3241 gcttgacaat ctccagcgtc ttggtttcat cgatgacctc tgcatccaac atgtctcgta
     3301 tggagtcgaa ctcaaagatg atctgcttga accaaagctc gtaggctgtg gcgaaggtac
     3361 ttaaatgcca ttgagtgttg tcatcaaagt tgtaaaccta ctcaccctgg tgcgtgatga
     3421 tgaacagatg ctcatcgtgc acgggtcgct tgtcctcctc ggacagcata cactgggcat
     3481 ccagcagttt gtccagcatc agatactctc catagatttt gcccacttcc gtggttaatg
     3541 gcaccgccga atcatcgtga tcgtttctgt atgggtttga attgaatcgc agaactgaag
     3601 atcgattggc attcctggac agcacgtgct ggtgctcacc cgtttcctgc atagggacag
     3661 ctcatggtgc acagctcaga tcagatcgtg actcctcgag cggcggatgc tggcgaactg
     3721 atctccgcca gcggaccgga gatgagaccc cagcgaaccg ataacagagc gagagagctc
     3781 cagttccgac tgattgcaca gtcggtgatc tgggcgatgg gcactgccag ataggctggg
     3841 aattatcaat cacttgaggt gaaagtgcgg cgcacacaaa tnaagcttca aattattaaa
     3901 aataaaactt taaaaataat ttcgtctaat taatattatg agttaattca aaccccacgg
     3961 acatgctaag ggttaatcaa caatcatatc gctgtctcac tcagactcaa tacgacactc
     4021 agaatactat tcctttcact cgcacttatt gcaagcatac gccgacggga ccaccttatg
     4081 ttatttcatc atgggtaccc agcttttgtt ccctttagtg agggttaatt ccgagcttgg
     4141 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     4201 acataggagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg aggtaactca
     4261 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     4321 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     4381 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     4441 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     4501 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     4561 ggctcggccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4621 cgacaggact ataaagatac caggcgttcc cccctggaag ctccctcgtg cgctctcctg
     4681 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4741 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4801 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     4861 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     4921 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     4981 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5041 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5101 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5161 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5221 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     5281 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     5341 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actgcccgtc gtgtagataa
     5401 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5461 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5521 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5581 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     5641 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     5701 ttacatgatc ccccatgttg tgaaaaaaag cggttagctc cttcggtcct ccgatcgttg
     5761 tcagaagtaa gttggccgca gtgttatcac tcatgcttat ggcagcactg cataattctc
     5821 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     5881 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     5941 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6001 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6061 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6121 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6181 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6241 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     6301 ctgaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     6361 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     6421 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     6481 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     6541 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     6601 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     6661 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     6721 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctac
     6781 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaagg
     6841 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     6901 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg gagctc
//
