LOCUS       PGSvhEGFP3   7036 bp    DNA   circular  INV       04-FEB-2001
DEFINITION  pPGS_v_hs_EGFP_3.
ACCESSION   PGSvhEGFP3
VERSION
KEYWORDS    .
SOURCE      fruit fly.
  ORGANISM  Drosophila melanogaster
            Eukaryota; Metazoa; Arthropoda; Tracheata; Hexapoda; Insecta;
            Pterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
            Drosophilidae; Drosophila.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Gunnar,S. and Gunter,R.
  TITLE     Controlled expression of tagged proteins in Drosophila using a new
            modular P-element vector system
  JOURNAL   Mol Gen Genet 262 (6), 916-920 (2000)
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Gunnar,S. and Gunter,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2001) Institute of Genetics,
            Martin-Luther-University Halle, Weinbergweg 10, Halle 06120,
            Germany
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Drosophila melanogaster"
     misc_feature    1..541
                     /function="5' P repeat"
     misc_feature    542..1004
                     /function="hsp module"
     misc_feature    join(1005..1007,1749..1763)
                     /function="multi cloning site"
     misc_feature    1008..1748
                     /function="EGFP module"
     misc_feature    1764..2074
                     /function="3' polyA module"
     misc_feature    2075..3968
                     /function="vermilion module"
     misc_feature    3969..4173
                     /function="3' P repeat"
     misc_feature    4174..7036
                     /function="pBluescript II"
BASE COUNT     1921 a   1738 c   1715 g   1660 t      2 others
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gaaagccgca gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 atctagaaga atcccaaaac aaactggtta ttgtggtagg tcatttgttt ggcagaagaa
      601 aactcgagaa atttctctgg ccgttattcg ttattctctc ttttcttttt gggtctccct
      661 ctctgcacta atgctctctc actctgtcac acagtaaacg gcatactgct ctcgttggtt
      721 cgagagagcg cgcctcgaat gttcgcgaaa agagcgccgg agtataaata gagcgcttcg
      781 tctacggagc gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta
      841 agcaaataaa caagcgcagc tgaacaagct aaacaatctg cagtaaagtg caagttaaag
      901 tgaatcaatt aaaagtaacc agcaaccaag taaatcaact gcaactactg aaatctgcca
      961 agaagtaatt attgaataca agaagagaac tctgaatagg gcccatagcg gccgcatggt
     1021 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1081 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1141 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1201 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     1261 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1321 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1381 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1441 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     1501 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1561 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1621 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1681 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca aggcccgggc
     1741 aagtcgacga cgtcgctagc atacctaggt agtttaaatt gattgttgca ttcgtaaacc
     1801 gaaataaatg cctgtactag aacttccctt taaacctatt gtgcactagt tagtggtctc
     1861 acctggatct cccaaaataa cctcaactaa tgtctgtcaa aacacatcta cctccctttc
     1921 caggtgtcat ccagctgaag gcgctcttcg agggcaaaac cacgggtgcc agtatccagt
     1981 acaccacgac gcaacgaggc agggtgatgc tggtctacga gggttatcgg tatgtcgtga
     2041 atcgccagag cctgaagaac gtcttctgcc taggaagctt natttatttt gttatgttat
     2101 atgtattata tgtcagacat aaagaaaagg aacacatcaa atgtgataac aaagactaaa
     2161 caagtaattt tattacacca aaacgacaaa acagtaggca gaacaaacaa cgcatagcca
     2221 aacattgacc aattggatac cctgccgatt gtcagacact tttgttgatc agtttcttgc
     2281 gaatggtctc gtccagcggt ggaatcgcct cgcggggaat cagaaaagtg gacagattga
     2341 acagatccag aaacaccttg taccgatcac tgaaaccaaa aaaaaacaaa gggagaacag
     2401 tttgagttca ttgatccccg atataatcac atctgcgatg atcacctgag agtggagcgc
     2461 agatattgat atccagacga gccaccagtg cccaactgtt gggatccaat catgcgttgc
     2521 accatgatca cgtgattgtc tgcggcggga atagaaagta tttggttagg aaaaccagtc
     2581 ttaaacataa gatatattta taaaagagta tcaaagaatg caatacttac atctccactt
     2641 ggttattaac gagtcgatgt ccatgagcag ggtgagcaac tggtgtggtt ggctgaacct
     2701 gggttcatcc ctatagaagg tgatcatgat ggctccctga agggcacgat ggctaaaccg
     2761 gcgatcccca cgacgcacca gtgcatcgtg cactgccgga tcaaagatgg agcgatacac
     2821 ctcgcgtcgc ttctcaatgt ccatgaggcg gtagtttttc gccttctcca cgggctcctc
     2881 catggcgctc tgtacctgcg cctccaggaa tcgatcgacg ctctcctgaa acttggccca
     2941 gaagttgaag ccactctcct ccagtccggg cgtcctctcc agccatcgct gcactagctc
     3001 cagtagcgag ggatctttct ccgagttgcg aatcgaattc cgcgcctcct cgtcgctaaa
     3061 gacatccgag tacttctggt tgtatctcac ccgctgctct gtcagaactc ccagcttgtt
     3121 ctcgatcaaa cggaactgca gcgactgaaa accagatgcg ggtgccaggt acttgcggaa
     3181 gtccatgaag tctagcgggg tcatggtctc cagaatgggc acttggtcca ccaggagctg
     3241 tacaaaggaa gttataaacg gattttggta agagattcag aaagcactca cttttagaat
     3301 cagaaccact cggttcagtc gcttgacaat ctccagcgtc ttggtttcat cgatgacctc
     3361 tgcatccaac atgtctcgta tggagtcgaa ctcaaagatg atctgcttga accaaagctc
     3421 gtaggctgtg gcgaaggtac ttaaatgcca ttgagtgttg tcatcaaagt tgtaaaccta
     3481 ctcaccctgg tgcgtgatga tgaacagatg ctcatcgtgc acgggtcgct tgtcctcctc
     3541 ggacagcata cactgggcat ccagcagttt gtccagcatc agatactctc catagatttt
     3601 gcccacttcc gtggttaatg gcaccgccga atcatcgtga tcgtttctgt atgggtttga
     3661 attgaatcgc agaactgaag atcgattggc attcctggac agcacgtgct ggtgctcacc
     3721 cgtttcctgc atagggacag ctcatggtgc acagctcaga tcagatcgtg actcctcgag
     3781 cggcggatgc tggcgaactg atctccgcca gcggaccgga gatgagaccc cagcgaaccg
     3841 ataacagagc gagagagctc cagttccgac tgattgcaca gtcggtgatc tgggcgatgg
     3901 gcactgccag ataggctggg aattatcaat cacttgaggt gaaagtgcgg cgcacacaaa
     3961 tnaagcttca aattattaaa aataaaactt taaaaataat ttcgtctaat taatattatg
     4021 agttaattca aaccccacgg acatgctaag ggttaatcaa caatcatatc gctgtctcac
     4081 tcagactcaa tacgacactc agaatactat tcctttcact cgcacttatt gcaagcatac
     4141 gccgacggga ccaccttatg ttatttcatc atgggtaccc agcttttgtt ccctttagtg
     4201 agggttaatt ccgagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     4261 tccgctcaca attccacaca acataggagc cggaagcata aagtgtaaag cctggggtgc
     4321 ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     4381 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     4441 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     4501 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     4561 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     4621 gttgctggcg tttttccata ggctcggccc ccctgacgag catcacaaaa atcgacgctc
     4681 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgttcc cccctggaag
     4741 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     4801 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     4861 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     4921 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     4981 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     5041 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     5101 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     5161 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     5221 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     5281 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     5341 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     5401 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     5461 actgcccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     5521 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     5581 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     5641 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     5701 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     5761 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgaaaaaaag cggttagctc
     5821 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatgcttat
     5881 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     5941 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     6001 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     6061 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     6121 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     6181 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     6241 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     6301 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     6361 atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa aattcgcgtt
     6421 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     6481 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     6541 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     6601 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     6661 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     6721 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     6781 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     6841 ccattcgcca ttcaggctac gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     6901 attacgccag ctggcgaagg ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     6961 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
     7021 gggcgaattg gagctc
//
